Glyoxal and methylglyoxal as urinary markers of diabetes. Determination using a dispersive liquid–liquid microextraction procedure combined with gas chromatography–mass spectrometry

Pastor-Belda, Marta; Fernández-García, A.J.; Campillo, Natalia; Pérez-Cárceles, María D.; Motas, Miguel; Hernández‐Córdoba, Manuel; Viñas, Pilar

doi:10.1016/j.chroma.2017.06.041

Cited by 33 publications

(27 citation statements)

References 34 publications

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“…In the exhaustive paper, all the points of the developed protocol are described in detail and critically discussed. Pastor-Belda et al (2017) proposed an alternative method for G and MG measurements in urine samples. The sample preparation procedure consisted on salting-out assisted liquid-liquid extraction and dispersive liquid-liquid microextraction combined with gas chromatography-mass spectrometry (GC-MS).…”

Section: E Glycation Processes In Cultured Cells Animalmentioning

confidence: 99%

The role of mass spectrometry in studies of glycation processes and diabetes management

DaRonco

Crotti

Agostini

et al. 2018

Mass Spectrometry Reviews

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In the last decade, mass spectrometry has been widely employed in the study of diabetes. This was mainly due to the development of new, highly sensitive, and specific methods representing powerful tools to go deep into the biochemical and pathogenetic processes typical of the disease. The aim of this review is to give a panorama of the scientifically valid results obtained in this contest. The recent studies on glycation processes, in particular those devoted to the mechanism of production and to the reactivity of advanced glycation end products (AGEs, AGE peptides, glyoxal, methylglyoxal, dicarbonyl compounds) allowed to obtain a different view on short and long term complications of diabetes. These results have been employed in the research of effective markers and mass spectrometry represented a precious tool allowing the monitoring of diabetic nephropathy, cardiovascular complications, and gestational diabetes. The same approaches have been employed to monitor the non‐insulinic diabetes pharmacological treatments, as well as in the discovery and characterization of antidiabetic agents from natural products. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 38:112–146, 2019.

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Section: E Glycation Processes In Cultured Cells Animalmentioning

confidence: 99%

The role of mass spectrometry in studies of glycation processes and diabetes management

DaRonco

Crotti

Agostini

et al. 2018

Mass Spectrometry Reviews

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“…Table 4 summarize the analytical aspects of the proposed and the previously reported methods for analysis of α-DCs in biological fluids. In addition to the selectivity problem that was discussed previously, the reported methods suffered from several drawbacks including; long derivatization time (120-1440 min) [12][13][14][15]21], relatively large sample volumes (0.2-5 mL) [10][11][12][13]17,[19][20][21] and the use of complicated multistep extraction procedures [10][11][12][13]17,18]. On the other hand, the developed method needs only 30 min for completion of the derivatization reaction, used very small sample volume (0.025 mL) which compares only to the previously reported LC-MS/MS methods [14,16] which used expensive instrumentations that are not available in many laboratories, as well, the extraction of α-DCs from human serum was carried out using simple PPT procedure with very good recovery (91-105%).…”

Section: Comparison Of the Newly Developed Approach For Determinationmentioning

confidence: 99%

“…In 2006, a summary for the reported analytical methods for the determination of α-DCs in different matrices was reported by Shibamoto [4]. In the last ten years, many methods were reported for determination of α-DCs in biological fluids including: Gas chromatographic methods with flame ionization detection (GC-FID) [10,11] or with mass spectrometric detection (GC-MS) [12,13] and liquid chromatography with tandem MS (LC-MS/MS) [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, most of these methods depended on using α-diamino compounds as derivatizing reagents [10][11][12][13][14][15][16][17][19][20][21] which are not selective for α-DCs as they can react with any compound having aldehyde group in their structure forming imidazole derivatives [22] instead of the pyrazine derivative formed with the α-DCs. Also, CE-AD method depends on using the non-selective reagent, thiobarbituric acid, which is reactive towards many other compounds including malondialdehyde (MDA), reducing sugars, and pyrimidine [23].…”

Section: Introductionmentioning

confidence: 99%

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Aromatic aldehydes as selective fluorogenic derivatizing agents for α‐dicarbonyl compounds. Application to HPLC analysis of some advanced glycation end products and oxidative stress biomarkers in human serum

El-Maghrabey

Nakatani

Kishikawa

et al. 2018

Journal of Pharmaceutical and Biomedical Analysis

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α-Dicarbonyl compounds (α-DCs) are very clinically important as they are considered as advanced glycation end products (AGEs) precursors and biomarkers for many chronic diseases such as diabetes and vascular diseases, in addition to their major role in progression of complications of such diseases. Aromatic aldehydes and ammonium acetate were productively used as a one-pot co-reagents for fluorogenic derivatization of α-DCs yielding fluorescent imidazole derivatives. Among the tried aromatic aldehydes, 4-carbomethoxybenzaldehyde yielded the products with best fluorescent characters. This approach for fluorogenic derivatization of α-DCs overcome the selectivity problem of the most commonly used derivatization reagent for α-DCs, α-diamino compounds, that can react unselectively with α-DCs and aldehydes. Separation of the formed imidazole derivatives of five α-DCs including glucosone, 3-deoxyglucosone, glyoxal, methyl glyoxal and dimethyl glyoxal together with ethylmethylglyoxal as an internal standard was carried out on an octyl column using a mobile phase consisted of methanol-water (15:85, v/v%) containing 0.2% formic acid with time programed flow, followed by fluorescence detection at excitation/emission wavelengths of 310/410 nm. The method showed excellent sensitivity for the targeted α-DCs with limits of detections ranging from 0.4 to 5.0 nM in human serum. Simple protein precipitation procedure was used for human serum treatment yielding very good recovery (91-105%) for the targeted α-DCs. The developed method was fully validated, then applied to the analysis of the five above mentioned clinically important α-DCs in serum samples of healthy, diabetic, rheumatic and cardiac disorders human volunteers. Due to the excellent analytical features of the developed method, including high selectivity and sensitivity, it was able to detect the pattern of the targeted α-DCs serum levels under the investigated different clinical conditions.

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“…High levels of MGO occur when the concentrations of its precursors are elevated, for instance, during hyperglycemia (Allaman et al, 2015). Compared to healthy individuals, two-to four-fold higher levels of MGO is found in the plasma and urine diabetic patients (Odani et al, 1999;Lu et al, 2011;Senda et al, 2015;Pastor-Belda et al, 2017). MGO is one of the most potent glycating agents and is a potential precursor of advanced glycation end products (AGEs), which have been proposed as an important mechanism contributing to the pathophysiology of several complications in diabetes (Shamsaldeen et al, 2016;Hanssen et al, 2018), including diabetic nephropathy (Hanssen et al, 2019) and vascular dysfunction (Brouwers et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Methylglyoxal, a Reactive Glucose Metabolite, Induces Bladder Overactivity in Addition to Inflammation in Mice

et al. 2020

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Diabetic bladder dysfunction (DBD) is one of the most common complication of diabetes. Methylglyoxal (MGO), a highly reactive dicarbonyl compound formed as a by-product of glycolysis, is found at high levels in plasma of diabetic patients. Here, we explored the effects of chronic administration of MGO on micturition pattern (cystometry) and bladder contractility in vitro in healthy male C57/BL6 mice. Methylglyoxal was given at 0.5% in drinking water for 4 weeks. Exposure to MGO led to bladder tissue disorganization, edema of lamina propria, partial loss of urothelium and multiple leukocyte infiltrates. Filling cystometry revealed significant increases of micturition frequency and number of non-voiding contractions (NVCs) in the MGO group, clearly indicating an overactive bladder profile. Bladder contractions induced by electrical-field stimulation (EFS) and carbachol were significantly higher in the MGO group, while the muscarinic M 2 and M 3 mRNA expressions remained unchanged between groups. Additionally, MGO exposure induced upregulation of TRPA1 and down-regulation of TRPV1 and TRPV4 in bladder tissues. Methylglyoxal did not change the mRNA expression of the advanced glycation end products receptor (RAGE), but markedly increased its downstream NF-κB-iNOS signaling. The mRNA expression of cyclooxygenase-2 (COX-2) and reactive-oxygen species (ROS) levels remained unchanged. Altogether, our data show that 4-week MGO intake in mice produces an overactive bladder phenotype in addition to bladder inflammation and increased NF-kB/iNOS signaling. TRPA1 up-regulation and TRPV1/TRPV4 down-regulation may account for the MGO-induced bladder overactivity. Scavengers of MGO could be an option to ameliorate bladder dysfunction in diabetic conditions.

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Glyoxal and methylglyoxal as urinary markers of diabetes. Determination using a dispersive liquid–liquid microextraction procedure combined with gas chromatography–mass spectrometry

Cited by 33 publications

References 34 publications

The role of mass spectrometry in studies of glycation processes and diabetes management

The role of mass spectrometry in studies of glycation processes and diabetes management

Aromatic aldehydes as selective fluorogenic derivatizing agents for α‐dicarbonyl compounds. Application to HPLC analysis of some advanced glycation end products and oxidative stress biomarkers in human serum

Methylglyoxal, a Reactive Glucose Metabolite, Induces Bladder Overactivity in Addition to Inflammation in Mice

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