Glyphosate-induced antioxidant imbalance in HaCaT: The protective effect of Vitamins C and E

Gehin, Audrey; Guyon, Catherine; Nicod, Laurence

doi:10.1016/j.etap.2005.11.003

Cited by 56 publications

(39 citation statements)

References 43 publications

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“…Indeed, we were expecting morphological damages since the glyphosate concentrations of our study was in the same range as those of Malatesta et al (2008). In the same time, Marc et al (2004Marc et al ( , 2005 highlighted Roundup potential to inhibit transcription and cell division, in part responsible to the imbalance of the cell homeostasis and particularly the enzymatic and (Gehin et al 2005(Gehin et al , 2006. AFM images of surface of control human keratinocytes cell line showed multiple regularly densely packed crest-like protrusions, as previously described by Reich et al (2009).…”

Section: Discussionsupporting

confidence: 55%

“…We have previously demonstrated (Gehin et al 2005(Gehin et al , 2006 that glyphosate alone and included in Roundup formulations had cytotoxic effects on HaCaT cells in the range of 20 mM (approximately 4 mg/mL) after a 24h-treatment, inducing a biochemical disorder.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously demonstrated the susceptibility of human epidermal cells to glyphosate largely basing on oxidative stress process (Gehin et al 2005(Gehin et al , 2006 connected with an intracellular antioxidant impairment and an increased lipid peroxidation. These biochemical data led us to characterize morphological damages and histological disorders applied on the same model of cutaneous HaCaT cell line, qualifying the glyphosate treatment as a good model to induce oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

et al. 2009

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Among the molecules to which the human skin is exposed, glyphosate is used as an herbicide. Glyphosate has been shown to induce in vitro cutaneous cytotoxic effects, concomitant with oxidative disorders. In this following study, we focused on dynamic events of the loss of HaCaT cell integrity appearing after a glyphosate treatment. In these conditions, we showed that glyphosate is able to disrupt HaCaT cells and to induce intracellular oxidative cascade. In this aim, we optimized the conditions of cell treatment playing on exposure time (from 24 h to 30 min), which directly modify the cell viability profile (glyphosate 50% inhibition concentration from 28 to 53 mM) and allow to track cells along the treatment as an "induction and visualization" process. The combination of atomic force and fluorescence microscopic approaches offered opportunities to lead in parallel an investigation of the membrane surface and of the intracellular disorders, through cytoskeleton, nuclear, and oxidative stress marker targeting. The originality of our approach relies on monitoring all events derived from oxidative stress in process and performed by simultaneous cytotoxic induction and nanoscale cell visualization. We revealed a transition from spread and globular to elongated cell morphology, with a drastic cell size reduction, after a dose- and time-dependent glyphosate treatment; a redistribution of cell surface protrusions was also pointed out. All these membrane damages, added to observations of disorganized cytoskeleton, condensed chromatin, and overproduction of oxidative reactive species, lead us to conclude that glyphosate acts in induction of apoptotic process.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

et al. 2009

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“…Recently, it has been shown that GP induces oxidative stress in vitro in human cells. Therefore, degenerative neuronal processes could be produced, partly, for oxidative stress (Gehin et al 2006, Hultberg 2007.…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity and histopathological alterations of Roundup® herbicide on "cachama blanca" (Piaractus brachypomus)

2008

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Acute toxicity of the glyphosate -N (phosphonomethyl) glycine- herbicide, Roundup®, in juveniles of cachama blanca, (Piaractus brachypomus), was evaluated and the histopathological lesions were assessed. The 96 h lethal concentration 50 was 97.47mg.L-1 (P<0.05). In the gill, necrotic and proliferative lesions were detected. In the liver, congestion, degenerative foci, hyaline droplets and lipidic vacuolization of the hepatocytes were observed. In the stomach mild hyperplasia of mucous cells was detected, which was also observed in the skin. In this latter tissue, a large increase in the thickness of the epidermis with necrotic lesions, infiltration of leukocytes and melanin pigment were observed. In the brain, degenerative foci of neuronal bodies in the telencephalon associated with gliosis and infiltration of eosinophilic granule cells/mast cells were shown. In conclusion, gills, liver, skin and brain are susceptible to Roundup®. Moreover, effects on the central nervous system could affect olfaction as well as individual and group behavior, the reproductive performance of the fish and hence have repercussions at the population level.

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“…Par ailleurs, les résultats révèlent que les tiges de Ampelocissus multistriata contiennent des teneurs plus importantes en polyphénols totaux que les autres organes. Falleh et al (2006), Gehin et al (2006) …”

Section: Matériel Et Méthodesunclassified

Composes organiques et activités antioxydantes de <i>Ampelocissus multistriata</i> du Tchad

Nguinambaye¹,

Nana²,

Aly³

et al. 2015

J. App. Bioscience.

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RÉSUMÉObjectif : La présente étude est axée sur le dosage spectrophotométrique des composés organiques et phénoliques totaux dans les différents organes de Ampelocissus multistriata. Méthodologie et résultats : L'activité antioxydante des extraits a été évaluée par la méthode de FRAP. Le matériel utilisé est la matière sèche (MS) des feuilles, tiges, rhizomes, fruits et écorces. La vitamine C et l'acidité totale ont été dosées aussi bien dans le jus des fruits frais (MF) que dans les extraits des fruits et feuilles secs par titration. Les résultats y afférents ont montré que les taux de sucres varient d'un organe à un autre, mais les fruits et les tiges enregistrent les plus fortes teneurs respectivement 75,6% et 71,6% de MS. On observe par ailleurs un taux élevé de vitamine C dans le jus de fruit (4,9% de MF). En plus, les résultats ont montré que tous les organes sont riches en polyphénols totaux mais cela varie d'un organe à un autre. Les tiges et les écorces sont respectivement les plus riches en polyphénols de l'ordre de 550,74 et 471,4 mg équivalent acide gallique/g de MS. Le faible taux des polyphénols est enregistré dans les racines (216,9 mg équivalent acide gallique/g). Les tiges manifestent une activité antioxydante plus importante (77,93 mg d'équivalent de trolox/g) que les racines (34,54 mg d'équivalent de trolox/g). Les composés phénoliques ont contribué pour plus de 91% dans les activités antioxydantes des extraits. Conclusion et applications des résultats : Cette présente étude révèle une richesse en composés glucidiques, en vitamine C et en antioxydants. Ainsi, les résultats obtenus peuvent s'appliquer dans la recherche de nouveaux remèdes antioxydants aussi dans la recherche des additifs ou suppléments nécessaires dans la conservation des aliments. Mots clés: composés organiques, activité antioxydante, Ampelocissus multistriata. 8471 acidity were measured in both the fresh fruit juices (MF) in the extracts of fruits and dry leaves by titration. The results relating thereto have shown that sugar levels vary from one organ to another, but fruits and stems recorded the highest levels of 75.6% and 71.6% DM, respectively. Moreover, there is a high level of vitamin C in the fruit juice (4.9% MF). In addition, the results showed that all the organs are rich in total polyphenols but this varies from one organ to another. The stems and bark are respectively the richest in polyphenols of around 550.74 and 471.4 mg gallic acid equivalent / g DM. The low rate of polyphenols is stored in the roots (216.9 mg gallic acid equivalent / g). The stems show a higher antioxidant activity (77.93 mg equivalent of Trolox / g) that the roots (34.54 mg equivalent of Trolox / g). Phenolic compounds have contributed more than 91% in the antioxidant activities of the extracts. Conclusion and application of results: This present study reveals the high glucidic compounds, vitamin C and antioxidants. So,the results got can apply in the research of antioxidant new cure as in the research of additives or the necessary supplemen...

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Glyphosate-induced antioxidant imbalance in HaCaT: The protective effect of Vitamins C and E

Cited by 56 publications

References 43 publications

Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

Acute toxicity and histopathological alterations of Roundup® herbicide on "cachama blanca" (Piaractus brachypomus)

Composes organiques et activités antioxydantes de <i>Ampelocissus multistriata</i> du Tchad

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