Glyphosate induces autophagy in hepatic L8824 cell line through NO-mediated activation of RAS/RAF/MEK/ERK signaling pathway and energy metabolism disorders

Zhang, Linlin; Chen, Lu; Qi, Meng; Yu, Fuchang; Ni, Xiaotong; Hong, Haozheng; Xu, Haotian; Xu, Shiwen

doi:10.1016/j.fsi.2023.108772

Cited by 6 publications

(1 citation statement)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MAPK signaling pathway, which consists of RAS, RAF, MEK, and ERK, is involved in a variety of cellular response processes such as apoptosis, cell proliferation, and other processes [ 17 ]. There are three isoforms of RAF ( ARAF , BRAF , and CRAF ) that are phosphorylated by MEK [ 18 ]. The RAF family also has two pseudokinases, including a kinase inhibitor of Ras 1/2 (KSR1/2), which generally acts as a bridge between MEK and RAF and plays a key role in the activation of RAF [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA miR-212-5p Regulates the MEK/ERK Signaling Pathway by Targeting A-Raf proto-oncogene serine/threonine-protein kinase (ARAF) to Regulate Cowshed PM2.5-Induced NR8383 Apoptosis

Sun,

Jia

et al. 2023

Toxics

View full text Add to dashboard Cite

Objective: To investigate the role of miR-212-5p-targeted ARAF during the apoptosis of rat alveolar macrophages induced by cowshed PM2.5. Methods: miRNA and related target genes and pathways were predicted using the KEGG, TargetScan, and other prediction websites. NR8383 macrophages were treated with cowshed PM2.5 to establish an in vitro lung injury model in rats; meanwhile, for the assessment of cell viability, apoptosis, intracellular calcium ions, and mitochondrial membrane potential in NR8383 cells, RT-qPCR was used to detect the expression of miR-212-5p and the target gene ARAF. Results: The bioinformatic analyses showed that miR-212-5p and ARAF were involved in PM2.5-associated cellular damage. Exposure to different concentrations (0 μg/mL, 60 μg/mL, 180 μg/mL, 300 μg/mL) with different durations (0 h, 12 h, 24 h, 48 h) of cowshed PM2.5 resulted in apoptosis, increased intracellular calcium ions, and decreased mitochondrial membrane potential. The miR-212-5p mimic group showed an up-regulation of Bax and cleaved Caspase 3 expression but decreased Bcl2 expression compared to the NC group, and overexpression of ARAF up-regulated the expression of p-MEK1/2 and p-ERK1/2 and simultaneously reversed the above phenomena. Conclusions: miR-212-5p targets ARAF to affect the cowshed PM2.5-induced apoptosis through the MEK/ERK signaling pathway, providing a potential target for relevant farming industry and pathology studies.

show abstract

Section: Introductionmentioning

confidence: 99%