Glyphosate targets fish monoaminergic systems leading to oxidative stress and anxiety

Faria, Melissa; Bedrossiantz, Juliette; Ramírez, Jonathan Ricardo Rosas; Mayol, Marta; García, Gerardo Heredia; Bellot, Marina; Prats, Eva; García-Reyero, Natàlia; Gómez‐Canela, Cristian; Gómez-Oliván, Leobardo Manuel; Raldúa, Demetrio

doi:10.1016/j.envint.2020.106253

Cited by 68 publications

(36 citation statements)

References 57 publications

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“…While recent studies have increased concerns about the potential neurotoxicity of glyphosate and GBH [ 7 , 23 , 24 ], data regarding their effects on anxiety mechanisms are partial and fragmented. In this study, we investigated IEG expression in the anxiety-related brain regions of mice treated with sub-toxic doses of GBH with the aim to assess the neuronal basis of the behavioural signs and to disentangle relevant molecular mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Anxiety and Gene Expression Enhancement in Mice Exposed to Glyphosate-Based Herbicide

Ait-Bali

Kaikai

Ba‐M’hamed

et al. 2022

Toxics

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Growing evidence demonstrates that serotonin (5-HT) depletion increases activity in the amygdala and medial prefrontal cortex (mPFC), ultimately leading to anxiety behavior. Previously, we showed that glyphosate-based herbicides (GBHs) increased anxiety levels and reduced the number of serotoninergic fibers within the mPFCs and amygdalas of exposed mice. However, the impact of this 5-HT depletion following GBH exposure on neuronal activity in these structures is still unknown. In this study, we investigated the effects of GBH on immediate early gene (IEG) activation within the mPFCs and amygdalas of treated mice from juvenile age to adulthood and its subsequent effects on anxiety levels. Mice were treated for subchronic (6 weeks) and chronic (12 weeks) periods with 250 or 500 mg/kg/day of GBH and subjected to behavioral testing using the open field and elevated plus maze paradigms. Then, we analyzed the expression levels of c-Fos and pCREB and established the molecular proxies of neuronal activation within the mPFC and the amygdala. Our data revealed that repeated exposure to GBH triggers anxiogenic behavior in exposed mice. Confocal microscopy investigations into the prelimbic/infralimbic regions of the mPFC and in basolateral/central nuclei of the amygdala disclosed that the behavioral alterations are paralleled by a robust increase in the density and labelling intensity of c-Fos- and pCREB-positive cells. Taken together, these data show that mice exposed to GBH display the hyperactivation of the mPFC–amygdala areas, suggesting that this is a potential mechanism underlying the anxiety-like phenotype.

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Section: Discussionmentioning

confidence: 99%

Anxiety and Gene Expression Enhancement in Mice Exposed to Glyphosate-Based Herbicide

Ait-Bali

Kaikai

Ba‐M’hamed

et al. 2022

Toxics

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“…Here, an array of neurotransmitters can be monitored, as well as their precursors and degradation products, across different brain regions, providing a direct mechanistic link between pollutant‐induced behavioural changes and altered neuronal transmission (Mayol‐Cabré et al ., 2020). For example, pioneering research harnessing neurotransmitter profiling recently demonstrated direct effects of the pervasive herbicide glyphosate on fish monoaminergic systems, with resulting effects on oxidative stress and anxiety‐related behaviours (Faria et al ., 2021). Furthermore, significant recent breakthroughs mean that we can now dissect neural mechanisms for chemically induced brain activation and disruption using genetically encoded (bio)sensor systems coupled with high‐speed fluorescence light‐sheet microscopy (LSM; Winter et al ., 2017; Fig.…”

Section: Tools and Technologies For Advancing Behavioural Ecotoxicologymentioning

confidence: 99%

Frontiers in quantifying wildlife behavioural responses to chemical pollution

et al. 2022

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Animal behaviour is remarkably sensitive to disruption by chemical pollution, with widespread implications for ecological and evolutionary processes in contaminated wildlife populations. However, conventional approaches applied to study the impacts of chemical pollutants on wildlife behaviour seldom address the complexity of natural environments in which contamination occurs. The aim of this review is to guide the rapidly developing field of behavioural ecotoxicology towards increased environmental realism, ecological complexity, and mechanistic understanding. We identify research areas in ecology that to date have been largely overlooked within behavioural ecotoxicology but which promise to yield valuable insights, including within-and among-individual variation, social networks and collective behaviour, and multi-stressor interactions. Further, we feature methodological and technological innovations that enable the collection of data on pollutant-induced behavioural changes at an unprecedented resolution and scale in the laboratory and the field. In an era of rapid environmental change, there is an urgent need to advance our understanding of the real-world impacts of chemical pollution on wildlife behaviour. This review therefore provides a roadmap of the major outstanding questions in behavioural ecotoxicology and highlights the need for increased cross-talk with other disciplines in order to find the answers.

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“…Para os herbicidas, podem-se ressaltar os estudos realizados com glifosato, 2,4 D e atrazina (SANTOS et al 2015;CRUZ et al, 2016;RODEA-PALOMARES et al, 2015;HOU et al, 2017;TEIXEIRA et al, 2018). Neste contexto, o glifosato é o ingrediente ativo mais produzido e aplicado no mundo o que propicia sua presença em vários ecossistemas aquáticos (MARTINI et al, 2012;FARIA et al, 2021).…”

Section: Aspectos Gerais E Ambientais Sobre Os Agrotóxicosunclassified

“…Segundo Faria et al (2021), concentrações do herbicida glifosato presentes em muitos ecossistemas aquáticos podem prejudicar a sobrevivência dos peixes. Os autores citados constaram que concentrações ambientalmente relevantes do herbicida tem efeito neurotóxico no peixe D. rerio (FARIA et al, 2021).…”

Section: Ecotoxicologia E Organismos Bioindicadores De Agrotóxicosunclassified

Pesticides, water resources and bioindicators

Américo-Pinheiro

Mercado

2022

ANAP

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Pesticides are constantly used to control agricultural and urban pests. However, they can contaminate watercourses and intoxicate aquatic organisms. The ecotoxicological effects of these contaminants can be observed in studies with bioindicators. This article presents a literature review on the main environmental aspects associated with pesticides and their toxicity to aquatic bioindicators. The search for scientific publications was carried out on the platforms Science Direct, Scielo, Virtual University Library and Academic Google. The information found was complemented with aspects related to legislation on pesticides consulted on Government platforms. The occurrence of pesticides in water resources has been recorded in several countries around the world, which shows the constant and inadequate use of these substances. This issue brings to light major challenges that involve the search for preventive and/or recovery measures from possible impacts on the environment, non-target organisms and human beings who are exposed to contamination by pesticides. The use of pesticide contamination bioindicators is essential to identify the effects of these substances on the environment and the sensitivity between species and different aquatic trophic levels. Fish constitute an excellent group of bioindicators, among them the Danio rerio (zebrafish) is the one that stands out in ecotoxicological studies. The most frequently observed ecotoxicological effects in fish include lethality, neurotoxicity, and enzymatic, biochemical, hematological and histological changes. These effects can compromise the survival and health of these vertebrates, interfering with the balance of aquatic environments and possibly impacting human health.

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Glyphosate targets fish monoaminergic systems leading to oxidative stress and anxiety

Cited by 68 publications

References 57 publications

Anxiety and Gene Expression Enhancement in Mice Exposed to Glyphosate-Based Herbicide

Anxiety and Gene Expression Enhancement in Mice Exposed to Glyphosate-Based Herbicide

Frontiers in quantifying wildlife behavioural responses to chemical pollution

Pesticides, water resources and bioindicators

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