Glyphosatverwertung durch Pseudomonas sp. GS

Albrecht, Birgit; Stock, M.; Weidhase, R

doi:10.1016/s0232-4393(11)80156-3

Cited by 3 publications

(3 citation statements)

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“…Therefore this study confirms the previously reported findings that glyphosate is degraded in Pseudomonas spec. GS to phosphate and sarcosine (WEIDHASE et al 1990). Some of the phosphonates tested for utilization seem to be more stable than glyphosate or azophon (see Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…The microbial strain Pseudomonas spec. GS was isolated from soil (WEIDHASE et al 1990). The minimal medium for cultivation contains 0.4% sucrose and 0.01 % glycerol as carbon sources, 0.09% (10 mMol) sarcosine as nitrogen source and 0.017% (1 mMol) glyphosate or other phosphonates as source for phosphorus.…”

Section: Methodsmentioning

confidence: 99%

“…For investigation of phosphonate metabolism bacteria were cultivated as reported earlier (WEIDHASE et al 1990) using a starvation period for depletion of intrinsic phosphate levels. Metabolites of N-methylphosphonoanilines were identified and characterized by harvesting of 300 ml bacterial suspension in late logarithmic growth phase (centrifugation at 2500 g).…”

Section: Methodsmentioning

confidence: 99%

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In vivo utilization of N‐(phosphonomethyl)‐anilines and related substances by Pseudomonas spec. GS

Albrecht

Stock

Weidhase

1991

J. Basic Microbiol.

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Utilization of various phosphonates as source for phosphorus by the glyphosate degrading strain Pseudomonas spec. GS was investigated. Metabolites of phosphonate degradation were characterized indicating the cleavage of the C-P-bound as primary step of breakdown. The phosphonate N-(phosphonomethyl)-4'-nitroazobenzene-4-amine (azophon) was characterized as a suitable substrate for detection of C-P-bond splitting activity in vivo. Pseudomonas cells permeabilized by toluene treatment were also capable of phosphonate degradation whereas no in vitro activity of a putative C-P-bond cleaving enzyme was detectable after cell disruption.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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