Glypican-3 electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids peroxidase-like catalytic silver deposition

Graphene-Based Electrochemical Sensor for Detection of Hepatocellular Carcinoma Markers

“…An example of the aptamer-based biosensor is discussed here. Zhou et al (2020) found that the hemin/graphene nanohybrids (HGNs) can catalyze silver deposition to amplify the signal. They solidified GP73 aptamer as the capture probe, and assembled HGNs-aptamer (HGNs-Apt) as the signal probe.…”

Steric Hindrance-Mediated Enzymatic Reaction Enable Homogeneous Dual Fluorescence Indicators Aptasensing of Hepatocellular Carcinoma CTCs

“…The target substance (i.e., GPC3-positive CTCs) specifically binds with the 5′-biotin-modified aptamer (bioaptamer) to form a stable complex, opening the preformed dsDNA and releasing bio-P1 and bio-P2 ssDNA at physiological temperatures (Tables S1 and S2). 32 It is worth noting that the lymphocytes in the sample had almost no impact on the detection due to the lack of GPC3 expression. 33 Subsequently, SA (with a ratio of 1:4 to biotin) was added, and unopened double chains formed the aforementioned steric hindrance in 3′-OH, while ssDNA was not affected.…”

Current methods for the detection of glypican-3

“…Further, Z. Zhou et al combined rGO and hemin to construct hemin-functionalized graphene nanohybrids (HGNs), which have peroxidase-like activity. 58 In contrast to antibodies, aptamers are not immunogenic and toxic, can be prepared by chemical synthesis, and with aptamer-specific hydrogen bonding, aptamer–target complexes have very low dissociation constants, ranging from picomolar to nanomolar. Thus, aptamer-based sensors respond rapidly due to their excellent sensitivity and high affinity for their targets, making them promising for applications in disease diagnosis.…”