“…The blood samples were collected in the field and were refrigerated before dispatching them to the Human Genetics Laboratory of the Indian Statistical Institute, Calcutta, where they were analysed. ABO phenotype testing was done by agglutination tests in test tubes with anti-A and anti-B sera; and gammaglobulin (Gm and Km) groupings were done with anti-Gm-1, -2, -5, and Km-1 sera, following Steinberg (1980). Serum protein systems, haptoglobin (Hp), transferrin (TD, ceruloplasmin (Cp), and albumin (Alb) were studied by horizontal starch gel electrophoresis (Ashton and Beode, 1961), and the red cell systems, acid phosphatase (ACP), adenylate kinase (AK), and esterase D (EstD), were scored for genotype determinations by starch gel electrophoresis according to Harris and Hopkinson (1977).…”