“…GM-CSF and IL-8 were from R&D Systems (Minneapolis, MN); neutrophil-activating peptide-78 (ENA-78) and granulocyte chemotactic protein-2 (GCP-2) were from Cell Sciences (Norwood, MA); 20-trifluoro-LTB 4 was from Cayman Chemicals (Ann Arbor, MI); 24-well Transwell membrane tissue plates (with 6.5 mm diameter, 5-m-pore inserts) were from Corning Costar (Cambridge, MA); trypan blue, phalloidin-FITC conjugate, phalloidin-tetramethylrhodamine isothiocyanate (TRITC) conjugate, Amanita phalloides, 4Ј,6Ј-diamidino-2-phenylindole (DAPI), FMLP, protein kinase A inhibitor, calphostin, anti-rabbit IgG (agarose beads), the cAMP-dependent kinase inhibitor TTYADFIASGRTGRRNAIHD, antip70S6 kinase polyclonal Ab used for immunoblotting, and pertusssis toxin were from Sigma-Aldrich (St. Louis, MO); electrophoresis chemicals were from Bio-Rad (Richmond, CA); [␥- 32 P]ATP (30 Ci/mmol) and Percoll (density 1.131 g/ml) were from Amersham Pharmacia Biotech (Arlington Heights, IL); GelMount was from BioMeda-Fisher (Pittsburgh, PA); FACS FLOW buffer was from Fisher (Hanover Park, IL); rapamycin, LY294002, and wortmannin were from Calbiochem (San Diego, CA); anti-GM-CSF receptor, common  (CDw131)-chain, anti-GM-CSF, and anti-fibroblast growth factor (FGF) Abs were from BD Biosciences (Franklin Lakes, NJ); p70S6 kinase peptide substrate KKRNRT LTK was from Upstate Biotechnology (Lake Placid, NY); hamartin (60-kDa purified tagged fusion protein corresponding to aa 1-300 of hamartin of human origin) was from Santa Cruz Biotechnology (Santa Cruz, CA); and the BioPorter protein delivery reagent was purchased from Gene Therapy Systems (San Diego, CA).…”