GM1 and GD1a gangliosides modulate toxic and inflammatory effects of E. coli lipopolysaccharide by preventing TLR4 translocation into lipid rafts

Nikolaeva, Svetlana; Bayunova, L. V.; Sokolova, T. V.; Vlasova, Yu. A.; Bachteeva, Vera; Аврова, Н. Ф.; Парнова, Р. Г.

doi:10.1016/j.bbalip.2014.12.004

Cited by 38 publications

(23 citation statements)

References 71 publications

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“…It has been reported that it is possible that ganglioside enrichment could have an effect on TLR receptor conformation, leading to a decreased receptor affinity for ligand [49]. Gangliosides may induce prevention of TLR4 translocation into lipid rafts [58]. Our results show that gangliosides may also prevent other TLRs translocate into lipid rafts.…”

Section: Discussionmentioning

confidence: 60%

Ganglioside GD1a suppresses LPS-induced pro-inflammatory cytokines in RAW264.7 macrophages by reducing MAPKs and NF-κB signaling pathways through TLR4

Wang

Cui

Cao

et al. 2015

International Immunopharmacology

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Section: Discussionmentioning

confidence: 60%

Ganglioside GD1a suppresses LPS-induced pro-inflammatory cytokines in RAW264.7 macrophages by reducing MAPKs and NF-κB signaling pathways through TLR4

Wang

Cui

Cao

et al. 2015

International Immunopharmacology

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“…The ganglioside GM1 is a common component of membrane raft microdomains which is demonstrated to contain antiapoptotic and antiinflammatory properties (40). We found in resting cells a strong signal diffusely distributed in the cytoplasm ( Figure 4B, lower panel).…”

Section: Desipramine Abrogates Adamts13 Downregulation and Reveals Cymentioning

confidence: 57%

Acid Sphingomyelinase Promotes Endothelial Stress Response in Systemic Inflammation and Sepsis

Chung

Hupe

Otto

et al. 2016

Mol Med

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“…Many studies indicate that gangliosides may interact with components of the inflammatory response pathway such as NO [35][36][37]. In macrophages, it has been suggested that the inhibitory action of exogenous gangliosides on these cells is through the modulation of inducible NO synthase (iNOS) expression [38].…”

Section: No Formation Is Involved In the Expression/localization Of Smentioning

confidence: 99%

Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages

Vilcaes

Garbarino-Pico

Demichelis

et al. 2020

IJMS

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Gangliosides are constituents of the mammalian cell membranes and participate in the inflammatory response. However, little is known about the presence and enzymatic activity of ganglioside sialyltransferases at the cell surface of macrophages, one of the most important immune cells involved in the innate inflammatory process. In the present study, using biochemical and fluorescent microscopy approaches, we found that endogenous ST8Sia-I is present at the plasma membrane (ecto-ST8Sia-I) of murine macrophage RAW264.7 cells. Moreover, ecto-ST8Sia-I can synthetize GD3 ganglioside at the cell surface in lipopolysaccharide (LPS)-stimulated macrophages even when LPS-stimulated macrophages reduced the total ST8Sia-I expression levels. Besides, cotreatment of LPS with an inhibitor of nitric oxide (NO) synthase recovered the ecto-ST8Sia-I expression, suggesting that NO production is involved in the reduction of ST8Sia-I expression. The diminution of ST8Sia-I expression in LPS-stimulated macrophages correlated with a reduction of GD3 and GM1 gangliosides and with an increment of GD1a. Taken together, the data supports the presence and activity of sialyltransferases at the plasma membrane of RAW264.7 cells. The variations of ecto-ST8Sia-I and ganglioside levels in stimulated macrophages constitutes a promissory pathway to further explore the physiological role of this and others ganglioside metabolism-related enzymes at the cell surface during the immune response.

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GM1 and GD1a gangliosides modulate toxic and inflammatory effects of E. coli lipopolysaccharide by preventing TLR4 translocation into lipid rafts

Cited by 38 publications

References 71 publications

Ganglioside GD1a suppresses LPS-induced pro-inflammatory cytokines in RAW264.7 macrophages by reducing MAPKs and NF-κB signaling pathways through TLR4

Ganglioside GD1a suppresses LPS-induced pro-inflammatory cytokines in RAW264.7 macrophages by reducing MAPKs and NF-κB signaling pathways through TLR4

Acid Sphingomyelinase Promotes Endothelial Stress Response in Systemic Inflammation and Sepsis

Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages

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