GmAKT1 is involved in K+ uptake and Na+/K+ homeostasis in Arabidopsis and soybean plants

Wang, Xuesong; Zhao, Jialiang; Fang, Qingwei; Chang, Xingchao; Sun, Mingyang; Li, Wenbin; Li, Yongguang

doi:10.1016/j.plantsci.2020.110736

Cited by 29 publications

(14 citation statements)

References 75 publications

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“…In our study, it was determined that AKT1 , NHX1 andHKT1 gene expressions increased in leaf tissue in both lettuce genotypes with only NaCl application. Similar to our findings, these gene expressions have been reported to increase in cotton genotypes (NHX1 and AKT1 ) (Wang et al 2017), Malus hupehensis(AKT1 ) (Li et al, 2012), salt-resistant wheat and barley genotypes (AKT1 , NHX1 and HKT1 ) (Zeeshan et al, 2020), soybean (AKT1 ) (Wang et al, 2021), salt-tolerant and sensitive rice genotypes (NHX1 ) (Hossain et al, 2017), maize (HKT1 ) ( Zhang et al, 2018), tomato (NHX1 ) (Abdelaziz et al 2019), transgenic tobacco (HKT1 ) (Ali et al, 2019), and Arabidopsis (NHX1 ) (Krishnamurthy et al 2019), sorghum (NHX1 ) (Youssef et al, 2021) under salt stress. When our findings are examined for both genotypes in NaCl+MEL applications, it has been determined that AKT1 and NHX1 gene expressions increased in at the lowest MEL dose + NaCl aplication, HKT1 gene expression decreased in all of the applications.…”

Section: Discussionsupporting

confidence: 91%

“…It is thought that this damage caused by NaCl stress on the membranes is related to the high concentration of Na + ions accumulated in the apoplast by affecting the ionic bonds of structural elements such as pectin in the structure of the cell membrane or the apoplastic enzymes and disrupting the basic functions of the membrane (C ¸ulha and C ¸akırlar 2011). In addition, the deterioration of cell membrane structures due to membrane peroxidation by ROS whose accumulation increases in stress can lead to increased relative electrical conductivity (RC, Relative Electrical Conductivity) (Wang et al, 2021). Similar to our research results, the deterioration of membrane structures due to salt stress have reported in studies conducted on lettuce (Campos et al, 2019), barley (Zhang et al, 2022), pork loin (Dutta Gupta 2007), and tomato (Ali et al, 2021).…”

Section: Discussionsupporting

confidence: 89%

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THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

Taspinar

Kukara

Sığmaz

et al. 2023

Preprint

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Salinity, an important abiotic stress, affects arable land worldwide, reducing crop growth, yield and quality. Lettuce ( Lactuca sativa L.), which is very sensitive to salt stress, is one of the crops affected by soil salinity and salt (NaCl) stress also affects lettuce cultivation. Different plant growth regulators (salicylic acid (SA), polyamines, glycine betaine (GB) and melatonin (MEL)) are used to reduce the harmful effects of soil salinity. In this study, the effects of NaCl and MEL treatments on physiological parameters (cell membrane damage (%), leaf relative water content (RWC%), chlorophyll amount), DNA methylation and some NaCl tolerance genes ( SOS1, SOS2, AKT1, NHX1, HKT1) have examined in two different lettuce genotypes (Yedikule and Ivanka). When the effect of NaCl stress on physiological parameters was examined, it was detected that this stress increased cell membrane damage and decreased chlorophyll content and RWC %. The effects of MEL applications together with NaCl stress on these parameters were positive. In the gene expression analysis results, it was determined that SOS1, SOS2, AKT1, NHX1 and HKT1 gene expressions increased in both lettuce genotypes and decreased in MEL applications. On the flip side, according to methylation sensitive amplification polymorphism (MSAP) findings, while a decrease in methylation level was determined in NaCl stress compared to the control in both genotypes. Methylation levels increased compared to NaCl stress in MEL applications with NaCl stress, but this rate was still lower than the control. According to the results obtained, it was concluded that exogenous MEL application in lettuce genotypes is important in response to NaCl stress.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

Taspinar

Kukara

Sığmaz

et al. 2023

Preprint

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“…Notably, NHX2 increased greater in Magnum Salt than in Adrenalin. High-affinity K + channel (HKT) and a low-affinity K + channel Arabidopsis K + Transporter1 (AKT1) were reported to mediate K + /Na + influx ( Ali et al, 2021 , Wang et al, 2021 ). KAT3 encoded a member of the shaker family of voltage-gated potassium channel subunits and responded to low potassium stress in Arabidopsis, however, its function in salinity response had not been reported ( Wang et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Physiological and Transcriptome Profiles Uncover Salt Tolerance Mechanisms in Alfalfa

Sun

et al. 2022

Front. Plant Sci.

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Salinity is a major limiting factor that affects crop production. Understanding of the mechanisms of plant salt tolerance is critical for improving crop yield on saline land. Alfalfa (Medicago sativa L.) is the most important forage crop, while its salt tolerance mechanisms are largely unknown. The physiological and transcriptomic responses in two contrasting salt tolerant cultivars to salinity stress were investigated in the present study. “Magnum Salt” showed higher salt tolerance than “Adrenalin,” with higher relative germination rate, survival rate, biomass and K+/Na+ ratio after salt treatment. Activities of antioxidant enzymes SOD, CAT and GR, and proline concentrations were upregulated to higher levels in roots and shoots in Magnum Salt than in Adrenalin after salinity stress, except for no difference in GR activity in shoots, and lower levels of O2⋅– and H2O2 were accumulated in leaves. It was interesting to find that salinity caused a decrease in total unsaturated fatty acid in Adrenalin other than Magnum Salt, C18:2 was increased significantly after salinity in Magnum Salt, while it was unaltered in Adrenalin. High quality RNA sequencing (RNA-seq) data was obtained from samples of Magnum Salt and Adrenalin at different time points (0, 2, and 26 h). Generally, “phagosome,” “TCA cycle” and “oxidative phosphorylation” pathways were inhibited by salinity stress. Upregulated DEGs in Magnum Salt were specifically enriched in “fatty acid metabolism,” “MAPK signaling” and “hormone signal transduction” pathways. The DEGs involved in ionic homeostasis, reactive oxygen species (ROS) scavenging and fatty acid metabolism could partially explain the difference in salt tolerance between two cultivars. It is suggested that salt tolerance in alfalfa is associated with regulation of ionic homeostasis, antioxidative enzymes and fatty acid metabolism at both transcriptional and physiological level.

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“…CsAKT1 could be a target of nano-improved plant salt tolerance AKT1, as a class of inwardly rectifying K + channels, is widely existing in plants and is important for maintaining K + homeostasis. 54,55 It is a typical member of the Shaker family and plays an important role in plant response to salinity stress. Both the N-terminus and C-terminus of Shaker channel proteins are located in the cytoplasm.…”

Section: 2mentioning

confidence: 99%

CsAKT1 is a key gene for the CeO₂ nanoparticle's improved cucumber salt tolerance: a validation from CRISPR-Cas9 lines

Peng

Chen

Zhu

et al. 2022

Environ. Sci.: Nano

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GmAKT1 is involved in K+ uptake and Na+/K+ homeostasis in Arabidopsis and soybean plants

Cited by 29 publications

References 75 publications

THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

Comparative Physiological and Transcriptome Profiles Uncover Salt Tolerance Mechanisms in Alfalfa

CsAKT1 is a key gene for the CeO₂ nanoparticle's improved cucumber salt tolerance: a validation from CRISPR-Cas9 lines

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GmAKT1 is involved in K+ uptake and Na+/K+ homeostasis in Arabidopsis and soybean plants

Cited by 29 publications

References 75 publications

THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

THE EFFECT OF MELATONIN ON SOME PHYSIOLOGICAL PARAMETERS, SALT SENSOR GENES AND DNA METHYLATION IN SALT STRESSED LETTUCE ( Lactuca Sativa L.)

Comparative Physiological and Transcriptome Profiles Uncover Salt Tolerance Mechanisms in Alfalfa

CsAKT1 is a key gene for the CeO2 nanoparticle's improved cucumber salt tolerance: a validation from CRISPR-Cas9 lines

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CsAKT1 is a key gene for the CeO₂ nanoparticle's improved cucumber salt tolerance: a validation from CRISPR-Cas9 lines