GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants

Chen, Huatao; Chen, Xin; Gu, Heping; Wu, Bingyue; Zhang, Hongmei; Yuan, Xuesong; Cui, Xiaoyan

doi:10.1007/s10725-014-9890-3

Cited by 54 publications

(34 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This selective transport facilitates shoot to root recirculation of Na + and also decreases uptake of Na + from the root 12,13 . Recently, Chen et al 14 have found that a gene GmHKT1;4 in soybean regulates the Na + /K + ratio in root tissues and leads to salt tolerance in genetically engineered plants.…”

mentioning

confidence: 99%

Amelioration of Soybean Plant from Saline-Induced Condition by Exopolysaccharide Producing Pseudomonas-Mediated Expression of High Affinity K+-transporter (HKT1) Gene

et al. 2016

View full text Add to dashboard Cite

There is a consensus that soil salinization causes decreased agricultural production. Several plants may adapt to survive under high salt stress wherein glycophytes fail to grow. Among transporters in plants, the sodium transporter, also known as high-affinity K + -transporter (HKT1) that comes under the HKT gene family, is involved in uptake of sodium through the roots and its recirculation from shoot to root. In the present study, we have examined the role of transporter HKT1 in soybean plants upon addition of 200 mM NaCl and treatment with Pseudomonas sp. strain AK-1. It is reported that HKT1 is permeable to K + /Na + and systemically alleviates salinity stress through upregulation of gene expression in shoots and down-regulation in roots. Higher transcription levels in shoot recirculate Na + from shoot xylem to root phloem whereas lower transcription levels in root do not allow sodium to enter the plants through root cells. We have also examined role of exopolysaccharide (EPS) produced by strain AK-1 which helps in the binding of free Na + from soil and thus makes Na + unavailable to the soybean plants. Strain with EPS showed decrease in electrical conductivity of soil from 1.1 to 0.9 dS/m in the presence of 200 mM NaCl. In conclusion, treatment with Pseudomonas sp. strain AK-1 exhibits significant rise in shoot/root length, number of lateral roots, shoot/root fresh weight and decreased Na + /K + ratio under salinity stress.

show abstract

mentioning

confidence: 99%

Amelioration of Soybean Plant from Saline-Induced Condition by Exopolysaccharide Producing Pseudomonas-Mediated Expression of High Affinity K+-transporter (HKT1) Gene

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In rice, [22] demonstrated that HAK5 plays important roles in controlling both the in ux of K + into roots and its transport to the aerial parts of the plant. Two paralogs of our candidate gene (Glyma.02g154100 and Glyma.07g042500) were functionally characterized as being involved in the root uptake of K + in soybean [23,24]. Interestingly, in the work of Dhanapal et al [16], QTLs for K content do overlap with the genomic positions of these HAK5 paralogs.…”

Section: Candidates Genes and Their Functions For Mineral Elements Acmentioning

confidence: 86%

Identification of Loci Controlling Mineral Element Concentration in Soybean Seeds

Malle

Morrison

Belzile

2020

Preprint

View full text Add to dashboard Cite

Background: Mineral nutrients play a crucial role in the biochemical and physiological functions of biological systems. The enhancement of seed mineral content via genetic improvement is considered as the most promising and cost-effective approach compared alternative means for meeting the dietary needs. The overall objective of this study was to perform a GWAS of mineral content (Ca, K, P and S) in seeds of a core set of 137 soybean lines that are representative of the diversity of early maturing soybeans cultivated in Canada (maturity groups 000-II).Results: This panel of 137 soybean lines was grown in five environments (in total) and the seed mineral content was measured using a portable x-ray fluorescence (XRF) spectrometer. The association analyses were carried out using three statistical models and a set of 2.2 million SNPs obtained from a combined dataset of genotyping-by-sequencing and whole-genome sequencing. Eight QTLs significantly associated with the Ca, K, P and S content were identified by at least two of the three statistical models used (in two environments) contributing each from 17 to 31% of the phenotypic variation. A strong reproducibility of the effect of seven out these eight QTLs was observed in three other environments. In total, three candidate genes were identified involved in transport and assimilation of these mineral elements.Conclusions: There have been very few GWAS studies to identify QTLs associated with the mineral element content of soybean seeds. In addition to being new, the QTLs identified in this study and candidate genes will be useful for the genetic improvement of soybean nutritional quality through marker-assisted selection. Moreover, this study also provides details on the range of phenotypic variation encountered within the Canadian soybean germplasm.

show abstract

“…This phenomenon was observed in other SGGG-type HKT transporters. For instance, EcHKT1;1 showed K + permeability under low concentration of NaCl (Fairbairn et al 2000;Liu et al 2001), and OsHKT2;1 and GmHKT1;4 also show K + uptake ability (Ardie et al 2009;Yao et al 2010;Chen et al 2014). Other HKTs known to transport both K + and Na + , such as HvHKT2 (barley), TaH-KT2 (wheat), PhaHKT2 (reed), PutHKT2 (P. tenuiflora), and AlHKT2 (Aeluropus lagopoides), have been functionally characterized in yeast and/or oocytes systems (Benito et al 2014;Sanadhya et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Other HKTs known to transport both K + and Na + , such as HvHKT2 (barley), TaH-KT2 (wheat), PhaHKT2 (reed), PutHKT2 (P. tenuiflora), and AlHKT2 (Aeluropus lagopoides), have been functionally characterized in yeast and/or oocytes systems (Benito et al 2014;Sanadhya et al 2015). It has been shown that the Na + transport inhibition by K + in these class II transporters is attained at a certain K + threshold level (Horie et al 2001;Benito et al 2014;Chen et al 2014). This threshold level varies in different members of HKT class II based on the external NaCl concentration (Rubio et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+ uptake in Saccharomyces cerevisiae

Park¹,

Kou³

et al. 2017

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Soil salinity causes negative effects on the growth and yield of crops. In this study, two sweet potato (Ipomoea batatas. L) cultivars, Xushu 28 (X-28) and Okinawa 100 (O-100), were examined under 50 and 100 mmol L-1 NaCl stress. X-28 cultivar is relatively high salt tolerant than O-100 cultivar. Interestingly, real-time quantitative polymerase chain reaction (RT-qPCR) results indicated that sweet potato high-affinity K + transporter 1 (IbHKT1) gene expression was highly induced by 50 and 100 mmol L-1 NaCl stress in the stems of X-28 cultivar than in those of O-100 cultivar, but only slightly induced by these stresses in the leaves and fibrous roots in both cultivars. To characterize the function of IbHKT1 transporter, we performed ion-flux analysis in tobacco transient system and yeast complementation. Tobacco transient assay showed that IbHKT1 could uptake sodium (Na +). Yeast complementation assay showed that IbHKT1 could take up K + in 50 mmol L-1 K + medium without the presence of NaCl. Moreover, Na + uptake significantly increased in yeast overexpressing IbHKT1. These results showed that IbHKT1 transporter could have K +-Na + symport function in yeast. Therefore, the modes of action of IbHKT1 in transgenic yeast could differ from the mode of action of the other class I HKT1 transporters. Potentially, IbHKT1 could be used to improve the salt tolerance nature in sweet potato.

show abstract

GmHKT1;4, a novel soybean gene regulating Na+/K+ ratio in roots enhances salt tolerance in transgenic plants

Cited by 54 publications

References 34 publications

Amelioration of Soybean Plant from Saline-Induced Condition by Exopolysaccharide Producing <i>Pseudomonas</i>-Mediated Expression of High Affinity K<sup>+</sup>-transporter (HKT1) Gene

Amelioration of Soybean Plant from Saline-Induced Condition by Exopolysaccharide Producing <i>Pseudomonas</i>-Mediated Expression of High Affinity K<sup>+</sup>-transporter (HKT1) Gene

Identification of Loci Controlling Mineral Element Concentration in Soybean Seeds

Ipomoea batatas HKT1 transporter homolog mediates K+ and Na+ uptake in Saccharomyces cerevisiae

Contact Info

Product

Resources

About