GMO Safety Assessment-Feasibility of Bioassay to Detect Allelopathy Using Handy Sandwich Method in Transgenic Plants

Ishii, Keisuke; Kawaoka, Akiyoshi; Taniguchi, Toru

doi:10.5772/30894

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Allelopathic activities of transgenic tree plants have also been studied by this method using lettuce as the recipient (Ishii et al 2012). However, the effects of A. thaliana have not been reported.…”

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confidence: 99%

In vitro bioassay of allelopathy of <i>Arabidopsis thaliana</i> by sandwich method and protoplast co-culture method with digital image analysis

Sasamoto

Azumi

Shimizu

et al. 2017

Plant Biotechnology

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We examined the allelopathic activities of Arabidopsis thaliana, ecotype Columbia by two in vitro methods. The effect of dried leaves on the growth of recipient lettuce seedlings was examined by the sandwich method. The allelopathic activity on protoplast growth was examined by co-culture with recipient lettuce leaf protoplasts in 50 µl liquid medium using a 96-well culture plate. Non-spherically enlarged or dividing protoplasts of lettuce were counted under an inverted microscope. Inhibition of yellow accumulation during lettuce protoplast growth was quantitated by image analysis of scanned digital images of 96-well culture plates. The results were described as the percentages of control without A. thaliana. The results were compared with those obtained using several plants which had strong allelopathic activities on recipient lettuce using the same methods. The growth of lettuce protoplasts was inhibited at both 4 and 8 days of culture with protoplasts of A. thaliana. The results suggested the usefulness of the protoplast co-culture method for future studies on allelopathy.

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confidence: 99%

In vitro bioassay of allelopathy of <i>Arabidopsis thaliana</i> by sandwich method and protoplast co-culture method with digital image analysis

Sasamoto

Azumi

Shimizu

et al. 2017

Plant Biotechnology

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“…The inhibition of germination and early growth of seedlings is commonly observed as the main allelopathic effect [1]. Since the sandwich (SW) method, a small-scale bioassay, was established [2] [3], it has been widely used to determine the allelopathic potential of many plants, including bryophytes [4], transgenic aspen and eucalyptus [5], 178 Caucasian plant species [6], and medicinal plants [7].…”

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confidence: 99%

Cellular Growth Dynamics Affects Allelopathic Activity in Coffee Cell Culture

Asrori¹,

Ogita²

2023

AJPS

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Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared allelopathic activity of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L., Juniperus conferta, Prunus persica, Punica granatum, and Sonneratia ovata, using a modified "sandwich method bioassay" and found that coffee callus showed the strongest growth inhibition to lettuce seedling nearly 90% of hypocotyl and 96% of root. This coffee callus actively proliferated, with a 21-fold increase during five weeks of subculture, with a growth curve comprising two typical phases: a lag phase of 0 -2 weeks of culture and an exponential phase of 3 -5 weeks of culture. Allelopathic activity varied depending on the growth phase of the coffee callus. The strongest allelopathic activity was detected in 1 -2-week-old callus showing nearly 100% inhibitory effect on lettuce seedling growth. As the allelopathic activity of coffee calli is extremely high, beyond the natural level in coffee leaves and green beans, we focused on analyzing the allelopathic activity of its aqueous extracts using high-performance liquid chromatography. Several prominent peaks, including two reference alkaloids, theobromine and caffeine, which are known allelochemicals in coffee plants, and three distinct unknown peaks were identified at 270 nm in coffee calli during the lag phase (1 -2 weeks of culture). The higher value of the total phenolic content in the lag phase also suggested a key biosynthetic pathway in relation to the allelopathic activity of coffee callus will be activated in the lag phase.

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GMO Safety Assessment-Feasibility of Bioassay to Detect Allelopathy Using Handy Sandwich Method in Transgenic Plants

Cited by 2 publications

References 22 publications

In vitro bioassay of allelopathy of <i>Arabidopsis thaliana</i> by sandwich method and protoplast co-culture method with digital image analysis

In vitro bioassay of allelopathy of <i>Arabidopsis thaliana</i> by sandwich method and protoplast co-culture method with digital image analysis

Cellular Growth Dynamics Affects Allelopathic Activity in Coffee Cell Culture

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