GmWRKY21, a Soybean WRKY Transcription Factor Gene, Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

Han, Zhenzhen; Wang, Jinyu; Wang, Xinxin; Zhang, Xijia; Cheng, Yanbo; Cai, Zhandong; Nian, Hai; Ma, Qibin

doi:10.3389/fpls.2022.833326

Cited by 15 publications

(8 citation statements)

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“…Also, GmWRKY21-expressing transgenic Arabidopsis plants had increased root growth, lower MDA, and higher proline accumulation under AlCl 3 stress. These results suggest that other TFs may promote tolerance to Al stress through the STOP1-ALMT1 node as a part of the general abiotic stress response [57].…”

Section: Stop1/camta2/wrky Regulatory Nodementioning

confidence: 79%

Recent Updates on ALMT Transporters’ Physiology, Regulation, and Molecular Evolution in Plants

Dabravolski,

Isayenkov

2023

Plants

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Aluminium toxicity and phosphorus deficiency in soils are the main interconnected problems of modern agriculture. The aluminium-activated malate transporters (ALMTs) comprise a membrane protein family that demonstrates various physiological functions in plants, such as tolerance to environmental Al3+ and the regulation of stomatal movement. Over the past few decades, the regulation of ALMT family proteins has been intensively studied. In this review, we summarise the current knowledge about this transporter family and assess their involvement in diverse physiological processes and comprehensive regulatory mechanisms. Furthermore, we have conducted a thorough bioinformatic analysis to decipher the functional importance of conserved residues, structural components, and domains. Our phylogenetic analysis has also provided new insights into the molecular evolution of ALMT family proteins, expanding their scope beyond the plant kingdom. Lastly, we have formulated several outstanding questions and research directions to further enhance our understanding of the fundamental role of ALMT proteins and to assess their physiological functions.

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Section: Stop1/camta2/wrky Regulatory Nodementioning

confidence: 79%

Recent Updates on ALMT Transporters’ Physiology, Regulation, and Molecular Evolution in Plants

Dabravolski,

Isayenkov

2023

Plants

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“…The culture temperature was 26 °C and the light/darkness was 16 h/8 h ( Wang et al., 2019a ). The cultures were incubated in 0.5 mM CaCl 2 solution (containing 50 µM AlCl 3 ) for 0, 3, 6, 9, 12, 24, 36, 48, 60 and 72 h. With the incubation in 0.5 mM CaCl 2 with 0, 25, 50, 75, or 100 µM AlCl 3 (pH4.5) for 24 h, all the roots of soybean are taken as samples in Al dose-effect experiments ( Han et al., 2022 ). For tissue expression pattern, the samples of roots, stems, and leaves of soybean seedlings incubated in 0.5 mM CaCl 2 (50 µM AlCl 3 (pH4.5) for the experimental group, without AlCl 3 for the control group) for 60 h were taken and rapidly frozen in liquid nitrogen, and stored at -80°C ( Wang et al., 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…The pTF101-GmABR1 plasmid was transformed into agrobacterium tumefaciens GV3101 cells by heat shock method ( Li et al., 2022 ). Genetic transformation of Arabidopsis was subsequently performed using agrobacterium-mediated flower impregnation ( Han et al., 2022 ). After the obtained transgenic plants were identified, the T 3 transgenic positive lines with high expression of GmABR1 in root tip were selected for subsequent experiments including the analysis of its expression patterns response to Al stress and the phenotypes resistant to Al stress ( Liu et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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GmABR1 encoding an ERF transcription factor enhances the tolerance to aluminum stress in Arabidopsis thaliana

Wang

et al. 2023

Front. Plant Sci.

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The ethylene response factor (ERF) transcription factors, which is one of the largest transcription factor families in plants, are involved in biological and abiotic stress response and play an important role in plant growth and development. In this study, the GmABR1 gene from the soybean inbred line Zhonghuang24 (ZH24)×Huaxia 3 (HX3) was investigated its aluminum (Al) tolerance. GmABR1 protein has a conserved domain AP2, which is located in the nucleus and has transcriptional activation ability. The results of real-time quantitative PCR (qRT-PCR) showed that the GmABR1 gene presented a constitutive expression pattern rich in the root tip, stem and leaf tissues of HX3. After Al stress, the GmABR1 transcript was significantly increased in the roots. The transcripts of GmABR1 in the roots of HX3 treated with 50 µM AlCl3 was 51 times than that of the control. The GmABR1 was spatiotemporally specific with the highest expression levels when Al concentration was 50 µM, which was about 36 times than that of the control. The results of hematoxylin staining showed that the root tips of GmABR1-overexpression lines were stained the lightest, followed by the control, and the root tips of GmABR1 RNAi lines were stained the darkest. The concentrations of Al3+ in root tips were 207.40 µg/g, 147.74 µg/g and 330.65 µg/g in wild type (WT), overexpressed lines and RNAi lines, respectively. When AlCl3 (pH4.5) concentration was 100 µM, all the roots of Arabidopsis were significantly inhibited. The taproot elongation of WT, GmABR1 transgenic lines was 69.6%, 85.6%, respectively. When treated with Al, the content of malondialdehyde (MDA) in leaves of WT increased to 3.03 µg/g, while that of transgenic Arabidopsis increased from 1.66-2.21 µg/g, which was lower than that of WT. Under the Al stress, the Al stress responsive genes such as AtALMT1 and AtMATE, and the genes related to ABA pathway such as AtABI1, AtRD22 and AtRD29A were up-regulated. The results indicated that GmABR1 may jointly regulate plant resistance to Al stress through genes related to Al stress response and ABA response pathways.

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“…The toxic effects of highly Al 3+ ions concentration on plants are evident, particularly in the root tip, where Al 3+ toxicity is most pronounced and inhibits nutrient and water absorption [4]. Al stress increases the production of reactive oxygen species (ROS), by arousing lipid membrane peroxidation, protein oxidation and so on [5,6].…”

Section: Introductionmentioning

confidence: 99%

MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress

Liu,

Mo,

Sun

et al. 2023

Plants

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The toxicity of aluminum (Al) in acidic soils poses a significant limitation to crop productivity. In this study, we found a notable increase in DnaJ (HSP40) expression in the roots of Al-tolerant alfalfa (WL-525HQ), which we named MsDjB4. Transient conversion assays of tobacco leaf epidermal cells showed that MsDjB4 was targeted to the membrane system including Endoplasmic Reticulum (ER), Golgi, and plasma membrane. We overexpressed (MsDjB4-OE) and suppressed (MsDjB4-RNAi) MsDjB4 in alfalfa hairy roots and found that MsDjB4-OE lines exhibited significantly better tolerance to Al stress compared to wild-type and RNAi hairy roots. Specifically, MsDjB4-OE lines had longer root length, more lateral roots, and lower Al content compared to wild-type and RNAi lines. Furthermore, MsDjB4-OE lines showed lower levels of lipid peroxidation and ROS, as well as higher activity of antioxidant enzymes SOD, CAT, and POD compared to wild-type and RNAi lines under Al stress. Moreover, MsDjB4-OE lines had higher soluble protein content compared to wild-type and RNAi lines after Al treatment. These findings provide evidence that MsDjB4 contributes to the improved tolerance of alfalfa to Al stress by facilitating protein synthesis and enhancing antioxidant capacity.

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GmWRKY21, a Soybean WRKY Transcription Factor Gene, Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

Cited by 15 publications

References 100 publications

Recent Updates on ALMT Transporters’ Physiology, Regulation, and Molecular Evolution in Plants

Recent Updates on ALMT Transporters’ Physiology, Regulation, and Molecular Evolution in Plants

GmABR1 encoding an ERF transcription factor enhances the tolerance to aluminum stress in Arabidopsis thaliana

MsDjB4, a HSP40 Chaperone in Alfalfa (Medicago sativa L.), Improves Alfalfa Hairy Root Tolerance to Aluminum Stress

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