GnIH and GnRH expressions in the central nervous system and pituitary of Indian major carp, Labeo rohita during ontogeny: An immunocytochemical study

Biswas, Saikat; Jadhao, Arun G.; Pinelli, Claudia; Palande, Nikhil V.; Tsutsui, Kazuyoshi

doi:10.1016/j.ygcen.2014.06.005

Cited by 39 publications

(68 citation statements)

References 29 publications

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“…The widespread distribution of GnIH-ir fibers suggests a multifunctional role for this peptide, as already suggested in other vertebrates Ubuka et al, 2008Ubuka et al, , 2009aTobari et al, 2010;Biswas et al, 2014]. Notably, the anti-FMRFamide antiserum in P. esculentus , the anti-NPFF antiserum in P. esculentus , R. perezi, and X. laevis [Pinelli et al, 1999;Chartrel et al, 2002;Crespo et al, 2003], and the anti-fGRP antiserum in L. catesbeianus [Koda et al, 2002] all identified the richest network of irpositive fibers in the median eminence, whereas we could reveal only a few GnIH-ir fibers in this region.…”

Section: Discussionsupporting

confidence: 62%

“…The distribution of GnIH-like immunoreactive (GnIH-ir) cells and fibers described here in the brain of the green frog P. esculentus was investigated using an antiserum directed against quail GnIH, which had already been used successfully for the immunohistochemical localization of GnIH in other vertebrates [Tsutsui et al, 2000;Ukena et al, 2003;Osugi et al, 2004;Kriegsfeld et al, 2006;Ubuka et al, 2008Ubuka et al, , 2009aAnjum et al, 2012;Biswas et al, 2014]. Although immunocytochemical techniques may pose molecular limitations, given the difficulty in distinguishing among structurally similar or related (like) molecules identified by antibodies that recognize shared epitopes, the use of a selective GnIH antiserum and cross-preadsorption tests with synthetic amphibian GnIH orthologous peptides indicates that the P. esculentus brain contains GnIH-like peptide-producing neurons.…”

Section: Discussionmentioning

confidence: 99%

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Neuroanatomical Organization of the Brain Gonadotropin-Inhibitory Hormone and Gonadotropin-Releasing Hormone Systems in the Frog Pelophylax esculentus

et al. 2014

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Growing evidence suggests that gonadotropin-inhibitory hormone (GnIH) may play a key role in mediating vertebrate reproduction. GnIH inhibits gonadotropin synthesis and release by decreasing the activity of gonadotropin-releasing hormone (GnRH) neurons as well as by directly regulating gonadotropin secretion from the pituitary. Whereas the presence of GnIH has been widely investigated in various classes of vertebrates, there are very few immunohistochemical reports focusing on GnIH in amphibians. The aim of this study was to assess the presence and neuroanatomical distribution of GnIH-like immunoreactivity in the brain of the anuran amphibian Pelophylax (Rana) esculentus (esculenta) and to explore any potential anatomical relationship with mammalian GnRH-immunoreactive (mGnRH-ir) elements. The GnIH-like immunoreactive (GnIH-ir) system constitutes two distinct subpopulations in the telencephalon and diencephalon, with the highest number of immunoreactive cells located in the preoptic and suprachiasmatic areas. GnIH-ir neurons were also observed in the medial septum, the anterior commissure, the dorsal hypothalamus, the periventricular nucleus of the hypothalamus, and the posterior tuberculum. Scattered GnIH-ir fibers were present in all major subdivisions of the brain but only occasionally in the median eminence. mGnRH-ir neurons were distributed in the mediobasal telencephalon, the medial septal area, and the anterior preoptic area. Double-label immunohistochemistry revealed that the GnRH and GnIH systems coexist and have overlapping distributions at the level of the anterior preoptic area. Some GnIH-ir fibers were in close proximity to mGnRH-ir cell bodies. Our results suggest that both the neuroanatomy and the functional regulation of GnRH release are conserved properties of the hypothalamic GnIH-ir system among vertebrate species.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Neuroanatomical Organization of the Brain Gonadotropin-Inhibitory Hormone and Gonadotropin-Releasing Hormone Systems in the Frog Pelophylax esculentus

et al. 2014

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“…These results were consistent with those obtained by qPCR, which revealed very high gnih expression in the diencephalon but also high gnih transcript levels in the olfactory bulbs/telencephalic hemispheres and the tegmentum of the mesencephalon. Previous studies have also reported the presence of Gnih cell populations in the olfactory/terminal nerve region in several teleosts (Sawada et al, ; Biswas et al, ; Di Yorio et al, ). In the sea bass, both immunohistochemical and laser‐capture microdissection techniques followed by qPCR, revealed that gnih mRNA is also present in these terminal nerve/olfactory Gnih‐ir cells, reinforcing that they represent real Gnih‐producing cells (Paullada‐Salmerón et al, ).…”

Section: Discussionmentioning

confidence: 83%

“…Contrary to that reported in tetrapods however, Gnih actions in fish reproduction appear contradictory between species and rather inconclusive. In the past years, Gnih precursors, their brain localization and/or their physiological effects have been characterized in cypriniforms (Zhang et al, ; Qi et al, 2013a, b; Moussavi et al ; Biswas et al ), salmoniforms (Amano et al, ), beloniforms (Zhang et al, ), tetraodontiforms (Ikemoto & Park, ; Zhang et al, ; Shahjahan et al, ; Shahjahan, Doi, and Ando, ), cichliforms (Biran et al, ; Ogawa et al, ; Di Yorio et al, ), and perciforms (Zhang et al, ; Wang et al, ; Choi, Habibi, & Choi, ; Choi et al ; Paullada‐Salmerón et al, 2016a, b, c). Although pleuronectiforms represent one of the most evolved teleost orders, with more than 600 species that exhibit not only ontogenetic and phylogenetic relevance but also economical interest, research aiming to characterize and analyze the Gnih system is absent in this order.…”

Section: Discussionmentioning

confidence: 99%

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

Aliaga-Guerrero

Paullada-Salmerón

Piquer

et al. 2017

J of Comparative Neurology

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Recently, gonadotropin-inhibitory hormone (GnIH) has emerged as an important regulator of reproduction in birds and mammals. This RFamide neuropeptide has neuromodulatory functions and controls the synthesis and/or release of gonadotropin-releasing hormone (GnRH) and gonadotropins. Although teleosts represent about half of all living vertebrates, scientific and technological advances on the Gnih system in fish are scarce, contradictory, and inconclusive. Research on the fish Gnih system appears necessary to better clarify its role in the neuroendocrine and environmental control of vertebrate reproduction. In this study, we cloned a full-length sequence for the Gnih precursor of a flatfish, the Senegalese sole, coding for three putative Gnih peptides (ssGnih). We also generated specific antibodies against these ssGnih peptides, and used them to localize Gnih cells and their projections in the brain and pituitary. The expression of gnih was particularly evident in the diencephalon, but also in the olfactory bulbs/cerebral hemispheres, optic tectum/tegmentum, retina, and pituitary. The three antibodies used provided consistent results and showed that ssGnih-immunoreactive perikarya were present in the olfactory bulbs, ventral telencephalon, caudal preoptic area, dorsal tegmentum and rostral rhombencephalon, and their fibers innervated the brain and pituitary profusely. Intramuscular injection of ssGnih-3 provoked a significant reduction in gnrh-3 and lh expression, whereas ssGnih-2 treatment did not affect transcript levels of the main reproductive genes. Our results reveal the existence of a functional Gnih system in the sole brain, profusely innervating different brain areas and the pituitary gland, which could represent an important factor in the neuroendocrine control of flatfish reproduction.

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“…Based mainly on evidence from studies on mammals and birds, the inhibitory influence of GnIH on reproduction is manifested, in part, through modulation of GnRH neuronal firing, GnRH-induced gonadotropin release, and inhibition of gonadotropin gene expression (Smith & Clarke 2010. Immunoreactive GnIH has also been detected in the pituitary of Indian major carp (Biswas et al 2014); however, recent work on teleosts has revealed that GnIH control of gonadotrope activities is complex and not always inhibitory. Goldfish (g) GnIH (LPXRFamide 3) exerts both stimulatory and inhibitory influences on basal goldfish gonadotrope functions in a season-dependent manner, as well as differentially modulating the effects of sGnRH-and cGnRHII-induced gonadotrope functions (Moussavi et al , 2013.…”

Section: Introductionmentioning

confidence: 99%

Seasonal effects of GnIH on basal and GnRH-induced goldfish somatotrope functions

Moussavi¹,

Wlasichuk²,

Chang³

et al. 2014

Journal of Endocrinology

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To understand how gonadotropin-inhibitory hormone (GnIH) regulates goldfish GH cell functions, we monitored GH release and expression during early, mid-, and/or late gonadal recrudescence. In vivo and in vitro responses to goldfish (g) GnIH were different, indicating direct action at the level of pituitary, as well as interactions with other neuroendocrine factors involved in GH regulation. Injection of gGnIH consistently reduced basal serum GH levels but elevated pituitary gh mRNA levels, indicating potential dissociation of GH release and synthesis. Goldfish GnRH (sGnRH and cGnRHII) injection differentially stimulated serum GH and pituitary gh mRNA levels with some seasonal differences; these responses were reduced by gGnIH. In contrast, in vitro application of gGnIH during 24-h static incubation of goldfish pituitary cells generally elevated basal GH release and attenuated sGnRH-induced changes in gh mRNA, while suppressing basal gh mRNA levels at mid-and late recrudescence but elevating them at early recrudescence. gGnIH attenuated the GH release responses to sGnRH during static incubation at early, but not at mid-and late recrudescence. In cell column perifusion experiments examining short-term GH release, gGnIH reduced the cGnRHII-and sGnRH-stimulated secretion at late recrudescence but inhibited tha action of cGnRHII only during mid-recrudescence. Interestingly, a reduction of basal GH release upon perifusion with gGnIH during late recrudescence was followed by a rebound increase in GH release upon gGnIH removal. These results indicate that gGnIH exerts complex effects on basal and GnRH-stimulated goldfish GH cell functions and can differentially affect GH release and mRNA expression in a seasonal reproductive manner.

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GnIH and GnRH expressions in the central nervous system and pituitary of Indian major carp, Labeo rohita during ontogeny: An immunocytochemical study

Cited by 39 publications

References 29 publications

Neuroanatomical Organization of the Brain Gonadotropin-Inhibitory Hormone and Gonadotropin-Releasing Hormone Systems in the Frog <b><i>Pelophylax esculentus</i></b>

Neuroanatomical Organization of the Brain Gonadotropin-Inhibitory Hormone and Gonadotropin-Releasing Hormone Systems in the Frog <b><i>Pelophylax esculentus</i></b>

Gonadotropin‐inhibitory hormone in the flatfish, Solea senegalensis: Molecular cloning, brain localization and physiological effects

Seasonal effects of GnIH on basal and GnRH-induced goldfish somatotrope functions

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