Gnotobiotic rainbow trout (<i>Oncorhynchus mykiss</i>) model reveals endogenous bacteria that protect against<i>Flavobacterium columnare</i>infection

Pérez-Pascual, David; Vendrell-Fernández, Sol; Audrain, Bianca; Bernal-Bayard, Joaquín; Patiño-Navarrete, Rafael; Petit, Vincent; Rigaudeau, Dimitri; Ghigo, Jean‐Marc

doi:10.1101/2020.06.19.161471

Cited by 4 publications

(4 citation statements)

References 71 publications

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“…Such models could confirm the protective effect of essential endogenous bacteria, during Y . ruckeri infection, since our results are mainly predictions and the causal effect can still be difficult to determine (Llewellyn et al, 2014; Pérez‐Pascual et al, 2021).…”

Section: Discussionmentioning

confidence: 85%

Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)

Rasmussen

Villumsen

Jørgensen

et al. 2022

Journal of Applied Microbiology

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Aims: Given the pivotal role played by the gut microbiota in regulating the host immune system, great interest has arisen in the possibility of controlling fish health by modulating the gut microbiota. Hence, there is a need to better understand of the host-microbiota interactions after disease responses to optimize the use of probiotics to strengthen disease resilience and recovery. Methods and Results:We tested the effects of a probiotic feed additive in rainbow trout and challenged the fish with the causative agent for enteric red mouth disease, Yersinia ruckeri. We evaluated the survival, host immune gene expression and the gut microbiota composition. Results revealed that provision of probiotics and exposure to Y. ruckeri induced immune gene expression in the host, which were associated with changes in the gut microbiota. Subsequently, infection with Y. ruckeri had very little effect on microbiota composition when probiotics were applied, indicating that probiotics increased stabilisation of the microbiota. Our analysis revealed potential biomarkers for monitoring infection status and fish health. Finally, we used modelling approaches to decipher interactions between gut bacteria and the host immune gene responses, indicating removal of endogenous bacteria elicited by nonspecific immune responses. Conclusions:We discuss the relevance of these results emphasizing the importance of host-microbiota interactions, including the protective potential of the gut microbiota in disease responses.

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Section: Discussionmentioning

confidence: 85%

Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)

Rasmussen

Villumsen

Jørgensen

et al. 2022

Journal of Applied Microbiology

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“…The microbiota is the microbial community playing a fundamental role in maintaining the health of teleost fish, mediating the interaction between the pathogen and the host immune system (13, 28). Since the immunostimulatory effect was not elicited by mucus mixed with AH alone but the mucus from AH-challenged fish, we hypothesized that the skin mucosal microbiota underwent the significant change by AH challenge.…”

Section: Resultsmentioning

confidence: 99%

“…Several Bacteria can serve as potential candidates in this buffering system by colony resistance and/or production of adherence-inhibitory compounds (33–35). Second, previous studies have shown that fish commensal microbiota can regulate the host immune system (28, 36–39). An immunostimulatory role was also found within the striped catfish skin mucus using the ex vivo skin model (Fig.…”

Section: Discussionmentioning

confidence: 99%

Aeromonas hydrophilainduces skin disturbance through mucosal microbiota dysbiosis inPangasianodon hypophthalmus

CHEN¹,

LIN²,

SIAO³

et al. 2022

Preprint

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Bacterial pathogens are well-equipped to adhere to and initiate infection in teleost fish. The fish skin mucus serves as the first barrier against environmental pathogens. The mucus harbors commensal microbes that form the microbiota, impacting the host physiological and immunological regulation. However, how the skin mucosal microbiota responds to the presence of pathogens remains largely unexplored. Thus, little is known about the status of skin mucus prior to the infection with noticeable symptoms. In this study, we aim to investigate the interaction between pathogen and skin mucosal microbiota, as well as the fish skin immune responses in the presence of pathogens. By challenging striped catfish (Pangasianodon hypophthalmus) with different concentrations of bacterial pathogen Aeromonas hydrophila (AH), the skin immune response and the mucosal microbiota were examined by qPCR and 16S rRNA gene sequencing analysis. We found a pathogen concentration able to stimulate the skin immune response associated with the significant mucosal microbiota change and re-confirmed with the ex vivo fish skin model. Further analysis indicated that the change was attributed to the significant increase in opportunistic pathogens over AH. We concluded that the presence and increase of AH results in dysbiosis of mucosal microbiota that can stimulate skin immune response. We believe our work can shed some light on host-pathogen-commensal microbiota interaction and therefore contribute to aquaculture infection prevention.

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“…Pérez -Pascual et al . 42 recently reported that Flavobacterium sp. strain 4466 inhibited the growth of F. columnare although the mechanism of the inhibition is not still clear.…”

Section: Discussionmentioning

confidence: 99%

Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum

Takeuchi

Fujiwara-Nagata

Katayama

et al. 2021

Sci Rep

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Rainbow trout fry syndrome (RTFS) and bacterial coldwater disease (BCWD) is a globally distributed freshwater fish disease caused by Flavobacterium psychrophilum. In spite of its importance, an effective vaccine is not still available. Manipulation of the microbiome of skin, which is a primary infection gate for pathogens, could be a novel countermeasure. For example, increasing the abundance of specific antagonistic bacteria against pathogens in fish skin might be effective to prevent fish disease. Here, we combined cultivation with 16S rRNA gene amplicon sequencing to obtain insight into the skin microbiome of the rainbow trout (Oncorhynchus mykiss) and searched for skin bacteria antagonistic to F. psychrophilum. By using multiple culture media, we obtained 174 isolates spanning 18 genera. Among them, Bosea sp. OX14 and Flavobacterium sp. GL7 respectively inhibited the growth of F. psychrophilum KU190628-78 and NCIMB 1947T, and produced antagonistic compounds of < 3 kDa in size. Sequences related to our isolates comprised 4.95% of skin microbial communities, and those related to strains OX14 and GL7 respectively comprised 1.60% and 0.17% of the skin microbiome. Comparisons with previously published microbiome data detected sequences related to strains OX14 and GL7 in skin of other rainbow trout and Atlantic salmon.

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Gnotobiotic rainbow trout (Oncorhynchus mykiss) model reveals endogenous bacteria that protect againstFlavobacterium columnareinfection

Cited by 4 publications

References 71 publications

Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)

Integrative analyses of probiotics, pathogenic infections and host immune response highlight the importance of gut microbiota in understanding disease recovery in rainbow trout (Oncorhynchus mykiss)

Aeromonas hydrophilainduces skin disturbance through mucosal microbiota dysbiosis inPangasianodon hypophthalmus

Skin bacteria of rainbow trout antagonistic to the fish pathogen Flavobacterium psychrophilum

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