Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Bezos, Javier; Casal, Carmen; Díez‐Delgado, Iratxe; Romero, Beatriz; Liandris, Emmanouil; Álvarez, Julio; Sevilla, Iker A.; Juan, Lucía de; Domı́nguez, Lucas; Gortázar, Christian

doi:10.1016/j.vetimm.2015.07.009

Cited by 33 publications

(32 citation statements)

References 19 publications

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“…The bacteriological culture results showed that both strains of M. tuberculosis persisted in cattle, at least over the 10 weeks of the infection time course. Interestingly, Bezos and colleagues also reported reduced pathological lesion scores for goats infected with M. tuberculosis as compared to infections with M. bovis or M. caprae 64 .…”

Section: Discussionmentioning

confidence: 99%

Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle

Villarreal‐Ramos

Berg

Whelan

et al. 2018

Sci Rep

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The Mycobacterium tuberculosis complex (MTBC) is the collective term given to the group of bacteria that cause tuberculosis (TB) in mammals. It has been reported that M. tuberculosis H37Rv, a standard reference MTBC strain, is attenuated in cattle compared to Mycobacterium bovis. However, as M. tuberculosis H37Rv was isolated in the early 1930s, and genetic variants are known to exist, we sought to revisit this question of attenuation of M. tuberculosis for cattle by performing a bovine experimental infection with a recent M. tuberculosis isolate. Here we report infection of cattle using M. bovis AF2122/97, M. tuberculosis H37Rv, and M. tuberculosis BTB1558, the latter isolated in 2008 during a TB surveillance project in Ethiopian cattle. We show that both M. tuberculosis strains caused reduced gross pathology and histopathology in cattle compared to M. bovis. Using M. tuberculosis H37Rv and M. bovis AF2122/97 as the extremes in terms of infection outcome, we used RNA-Seq analysis to explore differences in the peripheral response to infection as a route to identify biomarkers of progressive disease in contrast to a more quiescent, latent infection. Our work shows the attenuation of M. tuberculosis strains for cattle, and emphasizes the potential of the bovine model as a ‘One Health’ approach to inform human TB biomarker development and post-exposure vaccine development.

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Section: Discussionmentioning

confidence: 99%

Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle

Villarreal‐Ramos

Berg

Whelan

et al. 2018

Sci Rep

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“…Nevertheless, unlike previous reports, a characteristic feature identified in our case was the scarce development or absence of a fibrous capsule surrounding the granulomatous core of lesions. It is not clear if this particularity could be due to the host species, the mycobacterial species or even to the genotype of the strain involved, as different outcomes have been reported (Bezos et al, ; García‐Jiménez et al, ). The absence of fibrosis in lungs has been previously observed in a study of spontaneous latency in a rabbit model of pulmonary TB with M .…”

Section: Discussionmentioning

confidence: 99%

Tuberculosis outbreak caused byMycobacterium capraein a rabbit farm in Spain

Sevilla

Arnal

Fuertes

et al. 2019

Transbound Emerg Dis

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Animal tuberculosis remains a great source of socioeconomic and health concern worldwide. Its main causative agents, Mycobacterium bovis and Mycobacterium caprae, have been isolated from many different domestic and wild animals. Naturally, occurring tuberculosis is extremely rare in rabbits, and implication of M. caprae has never been reported earlier. This study describes a severe tuberculosis outbreak caused by M. caprae in a Spanish farm of rabbits raised for meat for human consumption. The disease was first identified in a cachectic dam, and then it was confirmed in ten does with similar clinical signs. Subsequently, a depopulation operation was ordered for public health, animal welfare and environmental reasons. To broaden knowledge of spontaneous tuberculosis in rabbits, a study focused on pathological, epidemiological and diagnostic aspects was carried out on 51 does and 16 kittens after receiving the necessary authorizations. These animals were subjected to a modified intradermal test. After being euthanized, rabbits were examined for the presence of visible tuberculosis‐compatible lesions. Lung, kidney, caecal appendix and sacculus rotundus samples underwent microbiological and anatomopathological analysis. Infection was revealed by at least one of the methods used in 71% of dams and in 44% of kittens. The intradermal test was shown to be a good indicator of infection. Lung was the tissue for which more animals were positive but renal and intestinal tissues were also affected in many cases. Apparently, M. caprae spread mainly through the aerogenous route. Infection was pathologically characterized by the absence of evident fibrous capsules surrounding granulomas. A spoligotype (SB0415) frequently found in this area was considered responsible for the outbreak but the source could not be established. Regardless of the exceptional nature of animal tuberculosis in this host, rabbit industry might not escape from its effects and therefore, current biosafety and surveillance strategies should also consider this disease.

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“…Goats are very sensitive to TB infection, exhibiting disease-causing lesions in the lungs similar to humans (41) when infected with different members of the MTBC, including M. bovis and M. caprae (42). Recent studies have demonstrated an effective and quick rate of TB transmission in this animal model by cohousing TB-infected and -noninfected goats (43).…”

Section: Animal Models To Study Tb Transmissionmentioning

confidence: 95%

Breaking Transmission with Vaccines: The Case of Tuberculosis

et al. 2017

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Members of the Mycobacterium tuberculosis complex (MTBC) have evolved causing tuberculosis (TB) in different mammalian hosts. MTBC ecotypes have adapted to diverse animal species, with M. bovis being the most common cause of TB in livestock. Cattle-to-human transmission of M. bovis through ingestion of raw milk was common before introduction of the pasteurization process. TB in humans is mainly caused by M. tuberculosis. This bacterium is considered a genetically clonal pathogen that has coevolved with humans due to its ability to manipulate and subvert the immune response. TB is a major public health problem due to airborne person-to-person transmission of M. tuberculosis. The essential yet unanswered question on the natural history of TB is when M. tuberculosis decides to establish latent infection in the host (resambling the lysogenic cycle of lambda phage) or to cause pulmonary disease (comparable to the lytic cycle of lambda phage). In this latter case, M. tuberculosis kills the host with the aim of achieving transmission to new hosts. Combating the TB epidemic requires stopping transmission. M. bovis BCG, the present vaccine against TB, is derived from M. bovis and only protects against disseminated forms of TB. Thus, a priority in TB research is development of new effective vaccines to prevent pulmonary disease. Attenuated vaccines based on M. tuberculosis as MTBVAC are potential candidates that could contribute to break the TB transmission cycle.

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Goats challenged with different members of the Mycobacterium tuberculosis complex display different clinical pictures

Cited by 33 publications

References 19 publications

Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle

Experimental infection of cattle with Mycobacterium tuberculosis isolates shows the attenuation of the human tubercle bacillus for cattle

Tuberculosis outbreak caused byMycobacterium capraein a rabbit farm in Spain

Breaking Transmission with Vaccines: The Case of Tuberculosis

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