Of the goby fishes, many
Gymnogobius
species have been poor recognized mainly because of the absence of enough molecular information and clear phylogenetic framework. In this study, the complete mitochondrial genome of
Gymnogobius petschiliensis
was determined and described. The mitogenome is 16,422 bp in length and consists of 22 tRNAs, 13 protein-coding genes, two rRNAs, one control region and a light strand replication origin (O
L
). The arrangement of this mitogenome is identical to that of the typical teleost. The overall base composition is 27.5%, 29.5%, 26.1%, and 16.9%, for A, T, C, and G, respectively, with a slight bias on A + T content (57.0%). The 13 protein-coding genes use the initiation codon ATG except
COI
, which uses GTG. Most of them use TAA or TAG as the stop codon, while
COII
,
COIII
, and
Cyt b
use an incomplete T or TA and
ND4
uses an unusual AGA. The maximum-likelihood phylogeny tree of 19 Gobionellinae species demonstrated that
G. petschiliensis
had a very closely relationship with the same genus
G. urotaenia
. This study is expected to contributing to the phylogenetic evolution of
G. petschiliensis
and further phylogenetic relationship of Gobionellinae and Gobiiformes.