Goblet cells and intestinal Alkaline phosphatase expression (IAP) during the development of the rat small intestine

Gomes, José Rosa; Ayub, Lais Costa; Reis, Camila Audrey dos; Machado, Miriam Joice; Silva, Jéssica da; Omar, Nádia Fayez; Soares, Maria Albertina de Miranda

doi:10.1016/j.acthis.2016.11.010

Cited by 12 publications

(8 citation statements)

References 29 publications

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“…Mucins play an important role in the protection of gut barrier function (11) . Gomes et al (33) have reported that the distribution of mucin-secreting goblet cell and IAP activity occurs during the development of the intestine in suckling and weaning mice. It has been demonstrated that mucin-deficient mice had impaired intestinal ALP expression and lipopolysaccharide detoxification activity (34) .…”

Section: Discussionmentioning

confidence: 99%

Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression ofIAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet

Okazaki

Katayama²

2018

Br J Nutr

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We have recently reported that soluble dietary fibre, glucomannan, increased colonic alkaline phosphatase (ALP) activity and the gene expression without affecting the small-intestinal activity and that colonic ALP was correlated with gut mucins (index of intestinal barrier function). We speculated that dietary fermentable carbohydrates including oligosaccharides commonly elevate colonic ALP and gene expression as well as increase mucin secretion and microbial fermentation. To test this hypothesis, male Sprague–Dawley rats were fed a diet containing 30 % lard with or without 4 % fructo-oligosaccharides (FOS), galacto-oligosaccharides (GOS), raffinose (RAF) and lactulose (LAC), which are non-digestible oligosaccharides or isomalto-oligosaccharides (IMOS; some digestible oligosaccharides) for 2 weeks. Colon ALP activity, the gene expression and gut luminal variables including mucins, organic acids and microbiota were measured. Colonic ALP was significantly elevated in the FOS, RAF and LAC groups, and a similar trend was observed in the GOS group. Colonic expression of intestinal alkaline phosphatase (IAP-I), an ALP gene, was significantly elevated in the FOS, GOS and RAF groups and tended to be increased in the LAC group. Dietary FOS, GOS, RAF and LAC significantly elevated faecal mucins, caecal n-butyrate and faecal ratio of Bifidobacterium spp. Dietary IMOS had no effect on colonic ALP, mucins, organic acids and microbiota. Colon ALP was correlated with mucins, caecal n-butyrate and faecal Bifidobacterium spp. This study demonstrated that non-digestible and fermentable oligosaccharides commonly elevate colonic ALP activity and the expression of IAP-I, with increasing mucins and microbial fermentation, which might be important for protection of gut epithelial homoeostasis.

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Section: Discussionmentioning

confidence: 99%

Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression ofIAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet

Okazaki

Katayama²

2018

Br J Nutr

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“…Our results are consistent with those reported by Gomes et al in demonstrating the temporal, spatial, and developmental relationship of IAP. 28 IAP is regulated by a variety of dietary, developmental, and hormonal factors. IAP regulation in the context of our data demonstrates that both mode of delivery and dietary selection impact IAP activity.…”

Section: Discussionmentioning

confidence: 99%

Factors Known to Influence the Development of Necrotizing Enterocolitis to Modify Expression and Activity of Intestinal Alkaline Phosphatase in a Newborn Neonatal Rat Model

Rentea

Biesterveld

et al. 2018

Eur J Pediatr Surg

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“…Therefore, during the first three weeks after birth, the mucosa layer, specially the epithelium, goes through several morphological and physiological remodeling related to digestive and absorptive processes. These functional changes are produced by a shift in the expression of different enzymes present in the cell membrane like lactase, maltase, sucrase and intestinal alkaline phosphatase (Goldstein et al, 1971; Uezato and Fujita, 1983;Tojyo, 1984; Gomes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

Reis

Gomes

2019

Preprint

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MMP-2 and MMP-9 are proteins with well stablished roles on the remodeling of tissue during morphogenesis. This study aimed to evaluate the activity and expression of the MMP-2 and MMP-9 in the rat small intestine mucosa layer on 15 th and 18 th days of intratuterine life (i.u.) and at 3rd, 10 th , 17 th, 25 th , and 32 th days after birth (a.b.). Samples were submitted to zimography, immunohistochemistry methods and Masson trichrome staining. Results showed that MMP-2 and MMP-9 were not expressed in the small intestine epithelium during intrauterine life.MMP-9 was immunolocalized in the villi goblet cells and in the lamina propria in cells identified as being the mast cells (a.b.). We concluded that in the i.u. and a.b. life the MMP-2 and MMP-9 were not expressed in the small intestine epithelium. However, after birth, because MMP-9 was expressed in the mast cels present in the lamina propria it may be involved in the remodeling process of the innate immunity that occurs during the small intestine development.key words: rat small intestine, MMP-2, MMP-9, development, mast cells During the suckling and weaning phases, the intestinal epithelium becomes completely developed, and two compartments can be identified; the villi (functional compartment) and the crypts, which are considered the proliferative compartment where cells are produced and differentiated, before their migration to the villi to execute their digestive functions.The suckling phase represents the period from birth until the 16th day of life, where milk is the main food. Around the 17th day, during the third week of life, diet with milk is changed to diet with solid food (weaning phase). Therefore, during the first three weeks after birth, the mucosa layer , specially the epithelium, goes through several morphological and physiological remodeling related to digestive and absorptive processes. These functional changes are produced by a shift in the expression of different enzymes present in the cell membrane like lactase, maltase, sucrase and intestinal alkaline phosphatase (Goldstein et al, 1971; Uezato and Fujita, 1983;Tojyo, 1984; Gomes et al., 2016).However, in this decade it has been demonstrated that another kind of enzymes denominated matrix metalloproteinases seem to have important roles during the development, growth and morphological maturation of different parts of the body like bones (Holmbeck et al, 1999; Yang et al., 2008), vascular system, skeletal muscle (Oh et al., 2004s), kidney and dental structures (Gomes et al., 2010, 2011, Omar et al., 2011, 2017.Metalloproteinases found in the extracellular matrix are denominated matrix metalloproteinases (MMPs) such as MMP-2 and MMP-9 (also denominated gelatinases) or may be anchored in the cell membrane which is denominated membrane matrix metalloproteinase (MT-MMPs). Despite this, there are still few descriptions about the involvement of MMPs in this process concerning to the development of the small intestine (Kurakata et al., 2008; Camargo et al., 2016) and none report regarding ex...

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Goblet cells and intestinal Alkaline phosphatase expression (IAP) during the development of the rat small intestine

Cited by 12 publications

References 29 publications

Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression ofIAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet

Consumption of non-digestible oligosaccharides elevates colonic alkaline phosphatase activity by up-regulating the expression ofIAP-I, with increased mucins and microbial fermentation in rats fed a high-fat diet

Factors Known to Influence the Development of Necrotizing Enterocolitis to Modify Expression and Activity of Intestinal Alkaline Phosphatase in a Newborn Neonatal Rat Model

Are the Matrix Metalloproteinases 2 and 9 beeing expressed in the mucosa of the rat small intestine during its intrauterine and postnatal life development ?

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