Gold atomic cluster mediated electrochemical aptasensor for the detection of lipopolysaccharide

Posha, Biyas; Nambiar, Sindhu R.; Sandhyarani, N.

doi:10.1016/j.bios.2017.10.030

Cited by 49 publications

(30 citation statements)

References 31 publications

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“…Aptamers to LPS were immobilized on gold electrodes modified with Au atomic clusters (0.5-2.0 nm in size) and the response was measured by the decrease in the current due to reduction of gold upon LPS binding [100]. A linear range from 0.01 aM-1.0 pM was found using differential pulse voltammetry and also with electrochemical impedance spectroscopy.…”

Section: Detection Of Lps Via Electrochemical Sensing Techniquesmentioning

confidence: 99%

Nanomaterials for Biosensing Lipopolysaccharide

2019

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Lipopolysaccharides (LPS) are endotoxins, hazardous and toxic inflammatory stimulators released from the outer membrane of Gram-negative bacteria, and are the major cause of septic shock giving rise to millions of fatal illnesses worldwide. There is an urgent need to identify and detect these molecules selectively and rapidly. Pathogen detection has been done by traditional as well as biosensor-based methods. Nanomaterial based biosensors can assist in achieving these goals and have tremendous potential. The biosensing techniques developed are low-cost, easy to operate, and give a fast response. Due to extremely small size, large surface area, and scope for surface modification, nanomaterials have been used to target various biomolecules, including LPS. The sensing mechanism can be quite complex and involves the transformation of chemical interactions into amplified physical signals. Many different sorts of nanomaterials such as metal nanomaterials, magnetic nanomaterials, quantum dots, and others have been used for biosensing of LPS and have shown attractive results. This review considers the recent developments in the application of nanomaterials in sensing of LPS with emphasis given mainly to electrochemical and optical sensing.

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Section: Detection Of Lps Via Electrochemical Sensing Techniquesmentioning

confidence: 99%

Nanomaterials for Biosensing Lipopolysaccharide

2019

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“…Lipopolysaccharides (LPS), often known as endotoxin, is a major ingredient of the outer membrane of gram-negative bacteria, which is greatly contributing to maintain cell wall integrity [103]. A ratiometric electrochemical aptasensor designed using Gold atomic cluster for sensitive lipopolysaccharide detection [105]. A ratiometric electrochemical aptasensor designed using Gold atomic cluster for sensitive lipopolysaccharide detection [105].…”

Section: Electrochemical Aptasensor For Detection Of Bacteriamentioning

confidence: 99%

“…Even minimal amount of endotoxin can cause fever, pyrogenicity, septic shock and may also lead to vascular blood clotting and multiorgan failure, so the detection of LPS is critical in medical, therapeutics and food security [104]. A ratiometric electrochemical aptasensor designed using Gold atomic cluster for sensitive lipopolysaccharide detection [105]. Atomic quantum clusters, with average particle size in the range of 0.5-2 nm are stable clusters and are considered as a missing link between single atom and plasmonic nanoparticles.…”

Section: Electrochemical Aptasensor For Detection Of Bacteriamentioning

confidence: 99%

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Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

Alizadeh

Salimi

2018

Electroanalysis

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This article reviews recent advances and developments in the field of bioaffinity electrochemical sensors with emphasis on a subclass including immunosensors, aptasensors, genosensors and molecularly imprinted sensosr. Recent insights into novel fabrication methodologies and electrochemical techniques have resulted in the demonstration of biosensors able to detection of wide range of target analyte. It has been shown that due to their high specificity and sensitivity, they represent a great tool in practical applications. Furthermore, at last couple decades nanotechnology has become very popular in the sensor fields due to the high capacity of nanomaterials to immobilize of bioaffinity agents and also their inhibiting effects on denaturation of enzymes and other bioaffinity systems, which they could well have beneficial effects for the performance of these sensors. The main focus of this review is to discuss methods for immobilizing bioreceptors onto a solid support, introduction of nanomaterials for improve sensitivity of biosensor and various electrochemical sensing platforms. The use of biosensor in these applications is most appropriate, because the analysis of trace substances in medical and healthcare applications, environmental science, and food industries is so important. In addition, recent advances in nanomaterial‐mediated bioaffinity sensors used for onset biosensing, point‐of‐care testing and healthcare management are also highlighted.

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“…Electrochemical measurements, such as electrochemical impedance spectroscopy, offer label-free, rapid, and sensitive detection-a real benefit towards point-of-care testing. For instance, detection limits close to single-molecule detection have been reported (lipopolysaccharide concentration of 0.01 attomolar) [8]. Herein, we focused on impedimetric aptamer sensors.…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

Siller

Preuß

Urmann

et al. 2020

Sensors

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Electrochemical spectroscopy enables rapid, sensitive, and label-free analyte detection without the need of extensive and laborious labeling procedures and sample preparation. In addition, with the emergence of commercially available screen-printed electrodes (SPEs), a valuable, disposable alternative to costly bulk electrodes for electrochemical (bio-)sensor applications was established in recent years. However, applications with bare SPEs are limited and many applications demand additional/supporting structures or flow cells. Here, high-resolution 3D printing technology presents an ideal tool for the rapid and flexible fabrication of tailor-made, experiment-specific systems. In this work, flow cells for SPE-based electrochemical (bio-)sensor applications were designed and 3D printed. The successful implementation was demonstrated in an aptamer-based impedimetric biosensor approach for the detection of Escherichia coli (E. coli) Crooks strain as a proof of concept. Moreover, further developments towards a 3D-printed microfluidic flow cell with an integrated micromixer also illustrate the great potential of high-resolution 3D printing technology to enable homogeneous mixing of reagents or sample solutions in (bio-)sensor applications.

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Gold atomic cluster mediated electrochemical aptasensor for the detection of lipopolysaccharide

Cited by 49 publications

References 31 publications

Nanomaterials for Biosensing Lipopolysaccharide

Nanomaterials for Biosensing Lipopolysaccharide

Ultrasensitive Bioaffinity Electrochemical Sensors: Advances and New Perspectives

3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of E. coli Crooks Strain

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