Gold based nano-photonic approach for point-of-care detection of circulating long non-coding RNAs

Shandilya, Ruchita; Kumari, Roshani; Singh, Radha Dutt; Chouksey, Anju; Bhargava, Arpit; Goryacheva, Irina Yu.; Mishra, Pradyumna Kumar

doi:10.1016/j.nano.2021.102413

Cited by 13 publications

(7 citation statements)

References 43 publications

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“…The lncRNAs are important regulatory RNA transcripts, known to circulate in different bodily fluids at differential levels in diseased and normal conditions. − As lncRNAs can potentially serve as a diagnostic biomarker, rapid screening of these circulating biomolecules has a remarkable clinical importance. Owing to this, we developed a simplified nanophotonic approach for the rapid, sensitive, and specific detection of lncRNAs.…”

Section: Resultsmentioning

confidence: 99%

Graphene Quantum-Dot-Based Nanophotonic Approach for Targeted Detection of Long Noncoding RNAs in Circulation

et al. 2022

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Recent progress in the field of nanophotonics has opened up novel avenues for developing nanomaterial-based biosensing systems, which can detect various disease-specific biomarkers, including long noncoding RNAs (lncRNAs) known to circulate in biological fluids. Herein, we designed and developed a nanophotonic approach for rapid and specific capture of lncRNAs using oligonucleotide-conjugated graphene quantum-dot-nanoconjugates. The method offers accurate identification of the target lncRNAs with high selectivity, despite the presence of other molecules in the given sample. The observations also pointed toward the high feasibility and simplicity of the method in the selective determination of lncRNAs. Overall, the approach has the potential of assessing lncRNA expression as a function of disease initiation and progression.

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Section: Resultsmentioning

confidence: 99%

Graphene Quantum-Dot-Based Nanophotonic Approach for Targeted Detection of Long Noncoding RNAs in Circulation

et al. 2022

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“…The obtained pellet was carefully collected and washed with 70% ethanol. The pellet that formed in the next step was resuspended in NFW (20 μL), set to air-dry, and then kept at −20 °C …”

Section: Methodsmentioning

confidence: 99%

“…The pellet that formed in the next step was resuspended in NFW (20 μL), set to air-dry, and then kept at −20 °C. 20 Preparation of the Streptavidin-Conjugated QDs. Three different QDs, both organic and inorganic types (Qdots 525, QD 705, and GQDs), were procured from ThermoFisher Scientific, Waltham, MA, USA, and Merch KGaA, Darmstadt, Germany.…”

Section: Rna Isolation and Expression Analysismentioning

confidence: 99%

Design and Fabrication of a Nanobiosensor for the Detection of Cell-Free Circulating miRNAS-LncRNAS-mRNAS Triad Grid

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Nazeer,

Bhargava

et al. 2023

ACS Omega

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The increased understanding of the competitive endogenous RNA (ceRNA) network in the onset and development of breast cancers has suggested their use as promising disease biomarkers. Keeping these RNAs as molecular targets, we designed and developed an optical nanobiosensor for specific detection of the miRNAs-LncRNAs-mRNAs triad grid in circulation. The sensor was formulated using three quantum dots (QDs), i.e., QD-705, QD-525, and GQDs. These QDs were surface-activated and modified with a target-specific probe. The results suggested the significant ability of the developed nanobiosensor to identify target RNAs in both isolated and plasma samples. Apart from the higher specificity and applicability, the assessment of the detection limit showed that the sensor could detect the target up to 1 fg concentration. After appropriate validation, the developed nanobiosensor might prove beneficial to characterizing and detecting aberrant disease-specific cell-free circulating miRNAs-lncRNAs-mRNAs.

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“…As biotin has a carboxylic acid, it can be covalently conjugated to many species. Biomolecules can be chemically prepared with an amine, thiol, or carboxyl-reactive biotin reagent to biotinylate them, or they can be genetically altered to have a biotin acceptor . Therefore, the (strept)avidin–biotin binding is one of the most substantial noncovalent interactions, approaching the strength of a covalent bond.…”

Section: Surface Conjugation Chemistrymentioning

confidence: 99%

“…Biomolecules can be chemically prepared with an amine, thiol, or carboxyl-reactive biotin reagent to biotinylate them, or they can be genetically altered to have a biotin acceptor. 85 Therefore, the (strept)avidin–biotin binding is one of the most substantial noncovalent interactions, approaching the strength of a covalent bond. As a result, the procedure is frequently referred to as the “covalent conjugation method”.…”

Section: Surface Conjugation Chemistrymentioning

confidence: 99%

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

et al. 2022

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Graphene quantum dots (GQDs) are carbonaceous nanodots that are natural crystalline semiconductors and range from 1 to 20 nm. The broad range of applications for GQDs is based on their unique physical and chemical properties. Compared to inorganic quantum dots, GQDs possess numerous advantages, including formidable biocompatibility, low intrinsic toxicity, excellent dispensability, hydrophilicity, and surface grating, thus making them promising materials for nanophotonic applications. Owing to their unique photonic compliant properties, such as superb solubility, robust chemical inertness, large specific surface area, superabundant surface conjugation sites, superior photostability, resistance to photobleaching, and nonblinking, GQDs have emerged as a novel class of probes for the detection of biomolecules and study of their molecular interactions. Here, we present a brief overview of GQDs, their advantages over quantum dots (QDs), various synthesis procedures, and different surface conjugation chemistries for detecting cell-free circulating nucleic acids (CNAs). With the prominent rise of liquid biopsy-based approaches for real-time detection of CNAs, GQDs-based strategies might be a step toward early diagnosis, prognosis, treatment monitoring, and outcome prediction of various non-communicable diseases, including cancers.

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Gold based nano-photonic approach for point-of-care detection of circulating long non-coding RNAs

Cited by 13 publications

References 43 publications

Graphene Quantum-Dot-Based Nanophotonic Approach for Targeted Detection of Long Noncoding RNAs in Circulation

Graphene Quantum-Dot-Based Nanophotonic Approach for Targeted Detection of Long Noncoding RNAs in Circulation

Design and Fabrication of a Nanobiosensor for the Detection of Cell-Free Circulating miRNAS-LncRNAS-mRNAS Triad Grid

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

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