The purposes of this study was to evaluate the ability of Aspergillus niger and Rhizopus oligosporus for one-step bioleaching of Cu and Pb in printed circuit boards (PCBs) scrap from e-waste recycling shops, compared to acidic extraction with citric and lactic acids. The fungal spore suspension was cultivated in potato dextrose broth with dried PCBs and a shaker for 42 days. Every 7 days the leachates were analyzed for Cu and Pb concentrations using atomic absorption spectroscopy. The Cu and Pb concentrations in PCBs in e-waste recycling shops were 152.81 ± 26.54 and 25.62 ± 8.33 g kg-1 PCBs, respectively. The leaching experiment showed that 0.05 M citric acid was the most efficient leaching pure acid: more than 54.59% of Cu and 79.55% of Pb was released into solution. Heavy metal leaching by the lactic acid was less efficient. The best metal bioleaching efficiency was achieved by A. niger fungus, which extracted approximately 46.