Gold-carbon nanoparticles mediated delivery of BSA: Remarkable robustness and hemocompatibility

Hameed, Mehavesh; Ahmady, Islam M.; Alawadhi, Hussain; Workie, Bizuneh; Sahle‐Demessie, Endalkachew; Cui, Han; Chehimi, Mohamed M.; Mohamed, Ahmed A.

doi:10.1016/j.colsurfa.2018.09.004

Cited by 27 publications

(20 citation statements)

References 32 publications

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“…42 In another study, the native gold nanoparticles and the BSA conjugated nanoparticles had no effect on the hemolysis of red blood cells. 43 All our recent studies indicate that gold-carbon nanoparticles and their bioconjugates are safe when uptaken by human cells. To confirm their cellular uptake, they were examined using confocal microscopy ( Figure 5)…”

Section: Cell Studiesmentioning

confidence: 99%

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

et al. 2020

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Gold nanoparticles coated with proteins have shown extraordinary biocompatibility which advanced to several nanomedicine engineering applications. We synthesized protein-coated gold nanoparticles using green and chemical reduction routes for cellular uptake study. In the current work, we coated gold-aryl nanoparticles of the type AuNPs-C 6 H 4-4-COOH with BSA, collagen, zein and lysozyme proteins. Both routes were carried out without phase-transfer catalysts or extraneous stabilizing agents. High crystallinity of the AuNPs synthesized by the green route can be seen in the transmission electron microscopy images. Osteosarcoma cancer cells are malignant bone tumors with abnormal cellular functions. Studies using MG-63 cells will provide mechanistic suggestions on the details of the amplification in tumors. We studied the cellular uptake of the bioconjugates by MG-63 osteosarcoma cells using laser confocal fluorescence microscopy (LCFM) and flow cytometry. In the LCFM study, BSA-AuNPs was uptaken most efficiently of all protein-coated gold nanoparticles synthesized by the green route. Zein and lysozyme coated nanoparticles, though small sizes, prepared by the green method were not efficiently uptaken by MG-63. The two nanoparticles are negatively charged and zein is also a hydrophobic coat. The difference in hydrophobicity and charge might have affected the internalization. All of those coated nanoparticles that were efficiently uptaken can potentially be used as diagnostic and therapeutic agents for osteosarcoma.

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Section: Cell Studiesmentioning

confidence: 99%

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

et al. 2020

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“…Similar NPs but capped with phenylcarboxylic groups and bovine serum albumin (BSA) were prepared in view of theranostics applications (Figure 4). [78] Another approach for the functionalization of Au NPs by diazonium salts takes advantage of so called "hot electrons", that can be produced on structured gold surfaces or nanoparticles by photochemical excitation of surface plasmons. These hot electrons, which are produced in nanoscale area of maximum near field enhancement, are able to reduce locally diazonium salts.…”

Section: Graphene Nanoplateletsmentioning

confidence: 99%

“…For example, remarkably stable hemocompatibility of Au NPs can be achieved by surface modification with BSA conjugates, via aryl diazonium salts. [78] Such hemocompatible NPs could be used as valuable nanotools in view of intravenous drug delivery. In this work, BSA conjugated Au NPs were generated by co-reduction of AuCl 4 and aryl diazonium salts bearing -COOH functional groups, using 9-borabicyclo(3.3.1)nonane as reducing agent, in the presence of BSA.…”

Section: Metallic and Oxide Nanoparticlesmentioning

confidence: 99%

Surface functionalization of nanomaterials by aryl diazonium salts for biomedical sciences

Luo

Onidas

et al. 2021

Advances in Colloid and Interface Science

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Nanoparticles (NPs) can be prepared by simple reactions and methods from a number of materials. Their small size opens up a number of applications in different fields, among which biomedicine, including: i) drug delivery, ii) biosensors, iii) bioimaging, iv) antibacterial activity. To be able to perform such tasks, NPs must be modified with a variety of functional molecules, such as drugs, targeting groups, chemical tags or antibacterial agents, and must also be prevented from aggregation. The attachment must be stable to resist during the transportation to the targeted location. Diazonium salts, which have been widely used for coupling applications and surface modification, fulfil such criteria. Moreover, they are simple to prepare and can be easily substituted with a large number of organic groups. This review describes the use of these compounds in nanomedicine with a focus on the construction of nanohybrids derived from metal, oxide and carbon-based NPs as well as viruses.

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“…PBS treatment was acted as the negative control, and deionized water treatment was adopted as the positive control. The hemolysis ratio was calculated by the following formula: hemolysis % = (sample absorbance-negative control absorbance )/(positive control absorbance -negative control absorbance) × 100% [31].…”

Section: Hemolysis Testmentioning

confidence: 99%

Rational Designed Rapid Liver Clearance Rare-earth Core-shell Nanoprobe for Dual-Modal Breast Cancer Imaging in the Second Near-Infrared Window

Wei

Duan²,

Huang³

et al. 2021

Preprint

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BackgroundFluorescence imaging as the beacon for optical navigation has wildly developed in preclinical studies due to its prominent advantages, including noninvasiveness and superior temporal resolution. However, the traditional optical methods based on ultraviolet (UV, 200-400 nm) and visible light (Vis, 400-650 nm) limited by their low penetration, signal-to-noise ratio, and high background auto-fluorescence interference. Therefore, the development of near-infrared-II (NIR-II 1000-1700 nm) nanoprobe attracted significant attentions toward in vivo imaging. Regrettably, most of the NIR-II fluorescence probes, especially for inorganic NPs, were hardly excreted from the reticuloendothelial system (RES), yielding the anonymous long-term circulatory safety issue. ResultsHere, we develop a facile strategy for the fabrication of Nd3+-doped rare-earth core-shell nanoparticles (Nd-RENPs), NaGdF4:5%Nd@NaLuF4, with strong emission in the NIR-II window. What’s more, the Nd-RENPs could be quickly eliminated from the hepatobiliary pathway, reducing the potential risk with the long-term retention in the RES. Further, the Nd-RENPs are successfully utilized for NIR-II in vivo imaging and magnetic resonance imaging (MRI) contrast agents, enabling the precise detection of breast cancer. ConclusionsThe rational designed Nd-RENPs nanoprobes manifest rapid-clearance property revealing the potential application toward the noninvasive preoperative imaging of tumor lesions and real-time intra-operative supervision.

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Gold-carbon nanoparticles mediated delivery of BSA: Remarkable robustness and hemocompatibility

Cited by 27 publications

References 32 publications

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

Surface functionalization of nanomaterials by aryl diazonium salts for biomedical sciences

Rational Designed Rapid Liver Clearance Rare-earth Core-shell Nanoprobe for Dual-Modal Breast Cancer Imaging in the Second Near-Infrared Window

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