Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli

Tamer, Uğur; Çetin, Demet; Suludere, Zekiye; Boyaci, İsmail Hakkı; Temiz, Havva Tümay; Yegenoglu, Hande; Daniel, Philippe; Dinçer, I.; Elerman, Y.

doi:10.3390/ijms14036223

Cited by 55 publications

(31 citation statements)

References 42 publications

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“…Nanoparticles of various shapes, sizes, and compositions have broad applications in microorganism detection [31,32]. Much attention has been focused on amplifying the detection signal using nanoparticles [33,34], which can enhance enzyme activity [35,36].…”

Section: Introductionmentioning

confidence: 99%

A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

Shen¹,

Hou²,

Jin³

et al. 2014

Gut Pathog

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This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL-1 in PBS and 6.8 × 102 to 6.8 × 103 CFU mL-1 in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method.

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Section: Introductionmentioning

confidence: 99%

A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

Shen¹,

Hou²,

Jin³

et al. 2014

Gut Pathog

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“…For scanning electron microscopic observations, bacterial cells interacted with antibody coated anisotropic magnetic nanoparticles were prepared according to our previous work [45]. Briefly, E. coli cells, which had interacted with nanoparticles, were separated from the solution with a magnet and washed three times to remove non specific bindings.…”

Section: Preparation Of Magnetic Gold Nanoparticle-antibody Conjugatementioning

confidence: 99%

Anisotropic core-shell Fe3 O4 @Au magnetic nanoparticles and the effect of the immunomagnetic separation volume on the capture efficiency

Zengi̇n¹,

Bozkurt

Boyaci

et al. 2014

Pure and Applied Chemistry

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The aim of this study was to synthesize in high product yield of anisotropic core-shell Fe 3 O 4 @Au magnetic nanoparticles and to investigate the effect of the immunomagnetic separation (IMS) volume on the capture efficiency. For these purposes and for the first time, we synthesized polyhedral magnetic nanoparticles composed of Fe 3 O 4 core Au shell. To synthesize magnetic gold anisotropic core-shell particles, the seed-mediated synthetic method was carried out. By choosing an appropriate amount of iron particles and growth solution the fine control of the seed-mediated approach is enabled. This led to the high product yield of anisotropic nanoparticles. The magnetic separation of these nanoparticles was easily accomplished, and the resulting nanoparticles were characterized with transmission electron microscopy (TEM), ultraviolet visible spectroscopy (UV-vis), near edge absorption fine structure (NEXAFS) spectroscopy, and X-ray diffraction (XRD). Additionally, the magnetic properties of the nanoparticles were examined. The magnetic nanoparticles (MNPs) were modified with antibody and interacted with Escherichia coli (E. coli). The high capture efficiency between the magnetic nanoparticles and E. coli is evidenced by SEM images. The capture efficiency decreases with an increase of volumes, and the highest capture efficiency was observed for E. coli in an experiment volume of 100 μL for magnetic nanoparticles. The percentage of captured E. coli for polyhedral nanoparticles was found to be approximately 95 % and for spherical nanoparticles 88 %, respectively.

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“…A variety of different approaches have been employed to protect this sensitive material from oxidation, where commonly used methods include coating with carbon [19,22], silica [23], noble metals and oxides [24][25][26], or the utilization of different surfactants and stabilizers [27]. However, none of these approaches lead to covalent grafting.…”

Section: Introductionmentioning

confidence: 99%

The convenient preparation of stable aryl-coated zerovalent iron nanoparticles

Guselnikova¹,

Galanov²,

Гутаковский³

et al. 2016

Nano Online

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A novel approach for the in situ synthesis of zerovalent aryl-coated iron nanoparticles (NPs) based on diazonium salt chemistry is proposed. Surface-modified zerovalent iron NPs (ZVI NPs) were prepared by simple chemical reduction of iron(III) chloride aqueous solution followed by in situ modification using water soluble arenediazonium tosylate. The resulting NPs, with average iron core diameter of 21 nm, were coated with a 10 nm thick organic layer to provide long-term protection in air for the highly reactive zerovalent iron core up to 180 °C. The surface-modified iron NPs possess a high grafting density of the aryl group on the NPs surface of 1.23 mmol/g. FTIR spectroscopy, XRD, HRTEM, TGA/DTA, and elemental analysis were performed in order to characterize the resulting material.1192

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Gold-Coated Iron Composite Nanospheres Targeted the Detection of Escherichia coli

Cited by 55 publications

References 42 publications

A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

Anisotropic core-shell Fe3 O4 @Au magnetic nanoparticles and the effect of the immunomagnetic separation volume on the capture efficiency

The convenient preparation of stable aryl-coated zerovalent iron nanoparticles

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