Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release

Paasonen, Lauri; Sipilä, Tuomas; Subrizi, Astrid; Laurinmäki, Pasi; Butcher, Sarah J.; Rappolt, Michael; Yaghmur, Anan; Urtti, Arto; Yliperttula, Marjo

doi:10.1016/j.jconrel.2010.07.095

Cited by 148 publications

(110 citation statements)

References 32 publications

Supporting

Mentioning

105

Contrasting

Order By: Relevance

“…This observation is in agreement with previous studies in which cytosolic drug release from photosensitive liposomes was triggered by light activation (43,44). Moreover, it could be argued that photosensitizer and light are solely producing damage and, thereby, providing danger signals that adjuvate cross-presentation and stimulation of CD8 T cell responses.…”

Section: Discussionsupporting

confidence: 93%

Photosensitizer and Light Pave the Way for Cytosolic Targeting and Generation of Cytosolic CD8 T Cells Using PLGA Vaccine Particles

Bruno

Waeckerle-Men

Håkerud

et al. 2015

The Journal of Immunology

View full text Add to dashboard Cite

The generation of CTLs is crucial in the immunological fight against cancer and many infectious diseases. To achieve this, vaccine Ags need to be targeted to the cytosol of dendritic cells, which can activate CD8 T cells via MHC class I (MHCI). Therefore, such targeting has become one of the major objectives of vaccine research. In this study, we aimed to bypass the unwanted and default MHC class II Ag presentation and trigger MHCI presentation by using a photosensitizer that, upon light activation, would facilitate cytosolic targeting of codelivered Ag. Poly(lactide-co-glycolide) microparticles ∼1 μm size were loaded with OVA and the photosensitizer tetraphenyl chlorine disulphonate (TPCS2a) and administered intradermally in mice, which were illuminated 1 d later for activation of the photosensitizer. Immunization in the presence of TPCS2a significantly increased activation of CD8 T cells compared with immunization without TPCS2a and as measured by CD8 T cell proliferation, production of proinflammatory IFN-γ, TNF-α, and IL-2, and prevention of tumor growth. Cytotoxicity was demonstrated by granzyme B production in vitro and by in vivo killing of CFSE-labeled targets. CD4-dependent Ab responses were abrogated in mice immunized with TPCS2a-containing particles, suggesting that photosensitization facilitated a shift from default MHC class II toward MHCI Ag presentation. Hence, vaccine particles with Ag and photosensitizers proved an effective vehicle or adjuvant for stimulation of CTLs, and they may find potential application in therapeutic cancer vaccination and in prophylactic and therapeutic vaccination against intracellular infections.

show abstract

Section: Discussionsupporting

confidence: 93%

Photosensitizer and Light Pave the Way for Cytosolic Targeting and Generation of Cytosolic CD8 T Cells Using PLGA Vaccine Particles

Bruno

Waeckerle-Men

Håkerud

et al. 2015

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In some reports, the encapsulation efficacy of prepared nanoparticle-loaded liposomes has not been reported [11,13] and this makes the comparison difficult. However, the encapsulation efficiency of our prepared nanoparticle-loaded liposomes is more than those reported by Sabaté et al [32] for similar nanoparticle concentration used to prepare magnetic nanoparticle-loaded liposomes.…”

Section: Encapsulation Efficiencymentioning

confidence: 99%

“…Park et al prepared gold nanoparticles of 3-4 nm loaded into the bilayer of liposomes and evaluated the fluidity changes of bilayer due to nanoparticle loading [10]. Also, Paasonen et al fabricated multilamellar liposomes with embedded gold nanoparticles of 2-4 nm [11]. In addition, there are some reports about the deposition of gold nanoparticles onto the surface of liposomes [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Magnetoplasmonic Liposome Carriers

Khosroshahi¹

2014

NSTOA

View full text Add to dashboard Cite

“…To date, most of the research relating to liposomes and gold nanoparticles has not investigated drug loading 17,[19][20][21] or has separately incorporated gold nanoparticles and free drug into liposomes. [22][23][24] Therefore, the present study investigated a light-responsive delivery system by incorporating druggold nanoparticle conjugates into liposomes, to enhance antitumor efficacy and reduce toxicity.…”

Section: Introductionmentioning

confidence: 99%

Delivery of vincristine sulfate-conjugated gold nanoparticles using liposomes: a light-responsive nanocarrier with enhanced antitumor efficiency

Liu¹,

He²,

Niu³

et al. 2015

IJN

View full text Add to dashboard Cite

Rapid drug release at the specific site of action is still a challenge for antitumor therapy. Development of stimuli-responsive hybrid nanocarriers provides a promising strategy to enhance therapeutic effects by combining the unique features of each component. The present study explored the use of drug–gold nanoparticle conjugates incorporated into liposomes to enhance antitumor efficiency. A model drug, vincristine sulfate, was physically conjugated with gold nanoparticles and verified by UV-visible and fourier transform infrared spectroscopy, and differential scanning calorimetry. The conjugates were incorporated into liposomes by film dispersion to yield nanoparticles (113.4 nm) with light-responsive release properties, as shown by in vitro release studies. Intracellular uptake and distribution was studied in HeLa cells using transmission electron microscopy and confocal laser scanning microscopy. This demonstrated liposome internalization and localization in endosomal–lysosomal vesicles. Fluorescence intensity increased in cells exposed to UV light, indicating that this stimulated intracellular drug release; this finding was confirmed by quantitative analyses using flow cytometry. Antitumor efficacy was evaluated in HeLa cells, both in culture and in implants in vivo in nude mice. HeLa cell viability assays showed that light exposure enhanced liposome cytotoxicity and induction of apoptosis. Furthermore, treatment with the prepared liposomes coupled with UV light exposure produced greater antitumor effects in nude mice and reduced side effects, as compared with free vincristine sulfate.

show abstract

Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release

Cited by 148 publications

References 32 publications

Photosensitizer and Light Pave the Way for Cytosolic Targeting and Generation of Cytosolic CD8 T Cells Using PLGA Vaccine Particles

Photosensitizer and Light Pave the Way for Cytosolic Targeting and Generation of Cytosolic CD8 T Cells Using PLGA Vaccine Particles

Fabrication and Characterization of Magnetoplasmonic Liposome Carriers

Delivery of vincristine sulfate-conjugated gold nanoparticles using liposomes: a light-responsive nanocarrier with enhanced antitumor efficiency

Contact Info

Product

Resources

About