Gold–Ferrocene Glyco‐Nanoparticles for High‐Sensitivity Electrochemical Detection of Carbohydrate–Lectin Interactions

Martos-Maldonado, Manuel C.; Thygesen, Mikkel B.; Jensen, Knud J.; Vargas‐Berenguel, Antonio

doi:10.1002/ejoc.201300205

Cited by 16 publications

(28 citation statements)

References 72 publications

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“…In a recent study, the binding of Con A to PAMAM dendrimers modified by mannopyranosyl ferrocene units was detected as a reduction in the peak current due to ferrocene oxidation using DPV [48]. The same group also developed mannopyranosyl ferrocene modified gold nanoparticles for DPV detection of Con A binding [49]. The use of quantum dots (ZnO) conjugated to a glycoprotein (CEA) was demonstrated to enable sensitive detection of CEA by competitive displacement of these conjugates from an electrode surface modified by lectin Con A, followed by SVW detection of zinc stripping peaks [50].…”

Section: Introductionmentioning

confidence: 99%

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Pandey

Bhattarai

Pornsuriyasak

et al. 2014

Journal of Electroanalytical Chemistry

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Electrochemical enzyme-linked lectinsorbent assays (ELLA) were developed using nanoporous gold (NPG) as a solid support for protein immobilization and as an electrode for the electrochemical determination of the product of the reaction between alkaline phosphatase (ALP) and p-aminophenyl phosphate (p-APP), which is p-aminophenol (p-AP). Glycoproteins or concanavalin A (Con A) and ALP conjugates were covalently immobilized onto lipoic acid self-assembled monolayers on NPG. The binding of Con A – ALP (or soybean agglutinin – ALP) conjugate to glycoproteins covalently immobilized on NPG and subsequent incubation with p-APP substrate was found to result in square-wave voltammograms whose peak difference current varied with the identity of the glycoprotein. NPG presenting covalently bound glycoproteins was used as the basis for a competitive electrochemical assay for glycoproteins in solution (transferrin and IgG). A kinetic ELLA based on steric hindrance of the enzyme-substrate reaction and hence reduced enzymatic reaction rate after glycoprotein binding is demonstrated using immobilized Con A–ALP conjugates. Using the immobilized Con A-ALP conjugate, the binding affinity of immunoglobulin G (IgG) was found to be 105 nM, and that for transferrin was found to be 650 nM. Minimal interference was observed in the presence of 5 mg mL−1 BSA as a model serum protein in both the kinetic and competitive ELLA. Inhibition studies were performed with methyl D-mannoside for the binding of TSF and IgG to Con A-ALP; IC50 values were found to be 90 μM and 286 μM, respectively. Surface coverages of proteins were estimated using solution depletion and the BCA protein concentration assay.

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Section: Introductionmentioning

confidence: 99%

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Pandey

Bhattarai

Pornsuriyasak

et al. 2014

Journal of Electroanalytical Chemistry

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“…All reagents were purchased from Sigma Aldrich and used without further purification. Compounds 2 [36], 3[31,32], 15 [36] and 16 [36] were synthesized as previously reported. Human galectin-3 was expressed and purified as previously reported [45].…”

Section: General Methodsmentioning

confidence: 99%

“…The mixture was refluxed for 30 min and cooled to room temperature before being filtered through a Sartorius Minisart 0.2 μm filter to provide citrate-stabilized AuNPs of 12.1 ± 1.0 nm of average diameter in a concentration of 16 nM, as determined by UV-vis spectroscopy [48] and TEM. The citrate-stabilized AuNPs solution was washed with 1 mM NaOH [36,49] by repetitive centrifugal filtration (10 kDa cut-off, 10× dilution) with three consecutive concentration and re-dissolution steps to obtain 16 nM AuNPs solution in 1 mM NaOH with an average size of 12.1 ± 1.2 nm as determined by TEM.…”

Section: Synthesis Of Citrate-stabilized Aunpsmentioning

confidence: 99%

“…The resulting azido-functionalized Fc-Lac 1 was then used for an additional Cu(I)-catalyzed AAC with alkyne-terminated PAMAM-dendrimers 9-11 to obtain Fc-Lac dendrimers 12-14 in 78-81% yields. Similarly, azido-functionalized Fc-Lac 1 reacted with alkynylated disulfide 16 [36] to obtain Fc-Lac disulfide 17 in a 95% yield. Alkyne-terminated PAMAM-dendrimers 9-11 and disulfide 16 were prepared via the reaction of the corresponding amino derivatives 6-8 and 15 with pent-4-ynoic anhydride [51] (Scheme 1).…”

Section: Synthesismentioning

confidence: 99%

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Multivalent Lactose–Ferrocene Conjugates Based on Poly(Amido Amine) Dendrimers and Gold Nanoparticles as Electrochemical Probes for Sensing Galectin-3

Martos-Maldonado

Quesada-Soriano

Garcı́a-Fuentes

et al. 2020

Preprint

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Galectin-3 is considered a cancer biomarker and bioindicator of fibrosis and cardiac remodeling, and, therefore, it is desirable to develop convenient methods for its detection. Herein, an approach based on the development of multivalent electrochemical probes with high galectin-3 sensing abilities is reported. The probes consist of multivalent presentations of lactose-ferrocene conjugates scaffolded on poly(amido amine) (PAMAM) dendrimers and gold nanoparticles. Such multivalent lactose-ferrocene conjugates are synthesized by coupling of azidomethylferrocene-lactose building blocks on alkyne-functionalized PAMAM, for the case of the glycodendrimers, and to disulfide-functionalized linkers that are then used for the surface modification of citrate-stabilized gold nanoparticles. The binding and sensing abilities towards galectin 3 of both ferrocene-containing lactose dendrimers and gold nanoparticles have been evaluated by means of isothermal titration calorimetry, UV-vis spectroscopy, and differential pulse voltammetry. The highest sensitivity by electrochemical methods to galectin-3 was shown by lactosylferrocenylated gold nanoparticles, which are able to detect the lectin in nanomolar concentrations.

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“…Alternatively, as one changes from the Au-core (say 150 nm average diameter) nanostructure to the SiO 2 -Au core-shell (say 120 nm SiO 2 -core diameter plus 15 nm Au-shell thickness) the SPR frequency shifts downward [64]. Indeed, interaction between the surface-moieties and the surface plasmon modes also shifts the SPR frequency [65]. The task of estimating the temperature upshift due to an ensemble of GNPs distributed throughout a targeted volume involves the calculation of the effective heat generated while the system is exposed to a given light source.…”

Section: Nanoparticle-based Hyperthermiamentioning

confidence: 99%

Remote Hyperthermia, Drug Delivery and Thermometry: The Multifunctional Platform Provided by Nanoparticles

Qi¹,

Morais

2014

J Nanomed Nanotechno

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The huge demand for biocompatible, robust, accurate and noninvasive technology to assess the temperature of a biological targeted site for monitoring the hyperthermia effect brings the topic of remote thermometry to a very high level of interest. There are already promising research directions to fulfil such demand in the short term and a review of the achievements in this issue is certainly worth. This report offers an overview of the research regarding the most promising nanothermometers nowadays, with emphasis on those addressed to remote operation while using optical or magnetic responses of nanosized materials as the thermometric property. More specifically the optical emission intensity, optical emission peak shift and optical emission lifetime will be covered as far as the optical-based nanothermometers are concerned. Additionally, for the magnetic-based nanothermometers the magnetization or the magnetic susceptibility are the thermometric properties covered in this review. Furthermore, the review includes the hyperthermia effect based on nanosized metallic or magnetic particles plus a couple of thermally-responsive polymeric drug delivery systems, aiming to provide an integrated view of the multipurpose platform offered by actuating-sensing nanoparticles. As far as the regulatory system is concerned the availability of noninvasive thermometry incorporating biocompatibility, robustness and accuracy will establish the grounds needed for the approval of the hyperthermia technology for therapeutic purposes, thus allowing the market of this technological option on a global scale.hyperthermia and thermoresponsive drug delivery systems for clinical use.Although nanothermometry is at its infancy considerable progress has been made recently in regard to its use for remote assessing the temperature at the site the nanomaterials are incorporated to. Nanoprobes allowing remote temperature-sensing using optical or magnetic properties are among the most promising directions nowadays. Most of the optical-based nanothermometers use the light-emitting intensity [15][16][17][18][19][20][21][22][23], light-emitting peak shift [24][25][26], or lifetime decay of a suitable optical band as the thermometric property [27][28][29][30]. As far as the clinical use is concerned the drawbacks of the actual optical-based nanothermometers rely on biocompatibility (usual semiconductor-based core nanoprobes or dye-based shell moieties are toxic), robustness (typical bleaching of organic-based shell moieties) or temperature accuracy (no better than 0.3°C nowadays), or even combination of these factors. Moreover, due to limitations imposed by living tissues on the optical penetration of light (optical therapeutic window in the 700-1100 nm wavelength range), noninvasive clinical use of optical excitation and signal pickup is a huge challenge, not yet solved, except for very special therapeutic applications, as for instance the photodynamic therapy for treatment (heating monitoring) of skin cancer and follow up [31]. Alternatively, ...

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Gold–Ferrocene Glyco‐Nanoparticles for High‐Sensitivity Electrochemical Detection of Carbohydrate–Lectin Interactions

Cited by 16 publications

References 72 publications

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Square-wave voltammetry assays for glycoproteins on nanoporous gold

Multivalent Lactose–Ferrocene Conjugates Based on Poly(Amido Amine) Dendrimers and Gold Nanoparticles as Electrochemical Probes for Sensing Galectin-3

Remote Hyperthermia, Drug Delivery and Thermometry: The Multifunctional Platform Provided by Nanoparticles

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