Gold-Hybridized Zinc Oxide Nanorods as Real-Time Low-Cost NanoBiosensors for Detection of virulent DNA signature of HPV-16 in Cervical Carcinoma

Abstract

Detection of host integrated viral oncogenes are critical for early and point-of-care molecular diagnostics of virus-induced carcinoma. However, available diagnostic approaches are incapable of combining both cost-efficient medical diagnosis and high analytical performances. To circumvent this, we have developed an improved IDE-based nanobiosensor for biorecognition of HPV-16 infected cervical cancer cells through electrochemical impedance spectroscopy. The system is fabricated by coating gold (Au) doped zinc oxide (ZnO) nanorods interfaced with HPV-16 viral DNA bioreceptors on top of the Interdigitated Electrode (IDE) chips surface. Due to the concurrently improved sensitivity and biocompatibility of the designed nanohybrid film, Au decorated ZnO-Nanorod biosensors demonstrate exceptional detection of HPV-16 E6 oncogene, the cancer biomarker for HPV infected cervical cancers. This sensor displayed high levels of sensitivity by detecting as low as 1fM of viral E6 gene target. The sensor also exhibited a stable functional life span of more than 5 weeks, good reproducibility and high discriminatory properties against HPV-16. Sensor current responses are obtained from cultured cervical cancer cells which are close to clinical cancer samples. Hence, the developed sensor is an adaptable tool with high potential for clinical diagnosis especially useful for economically challenged countries/regions.

Recent Advances in Zinc Oxide Nanoparticles (ZnO NPs) for Cancer Diagnosis, Target Drug Delivery, and Treatment

“…The sensor also has a durability of over 5 weeks, excellent repeatability, and great HPV-16 discriminating abilities. As a result, the proposed sensor is a versatile instrument with a lot of potential for clinical diagnosis, especially in economically challenged nations and areas [70].…”

Optical fiber sensors based on sol–gel materials: design, fabrication and application in concrete structures

“…Sol-gel materials have been extensively reported in bioelectrochemistry. 29,101 Several authors described the immobilization of biomolecules within functionalized sol-gel materials to construct biosensors 9,24 mostly due to their high biocompatibility. Besides all advantages, most sol-gel derived materials are electronic insulators with low charge-transfer efficiency and substrate diffusion.…”

“…Naturally, this has boosted the development of sol-gel materials, worldwide, particularly in biosensors, 28 and the fields of medicine and health care. 24,29 In the last few years, the development of OFS for application in the civil engineering area has gained significant attention. [10][11][12] Being a field extremely important in structural engineering, structural health monitoring (SHM) of infrastructures is becoming more and more relevant.…”

Reverse Electrochemical Sensing of FLT3-ITD Mutations in Acute Myeloid Leukemia Using Gold Sputtered ZnO-Nanorod Configured DNA Biosensors

“…The sensor adopts the use of interdigitated electrode (IDE) chips fabricated with an aptitude for sensitive recognition of the FLT3-ITD by employing a reverse-detection principle. IDE-based biosensors were chosen due to miniaturized nature of the device that offers multiple benefits such as portability, ease of modifications to the electrode surface, good reproducibility, low-cost, and reliable signal readouts [18]. Furthermore, the utilized IDE chip constitutes a nano-gapped electrode architecture which provides better sensitivity for nanomolecular analyte detections and faster signal responses compared with micro-gapped IDE chips or large cells of electrochemical measurements [19,20].…”