Gold Nano-Bio-Interaction to Modulate Mechanobiological Responses for Cancer Therapy Applications

Abstract

In the present study, we investigate the mechanobiological responses of human lung cancer that may occur through their interactions with two different types of gold nanoparticles: nanostars and nanospheres. Hyperspectral images of nanoparticle-treated cells revealed different spatial distributions of nanoparticles in cells depending on their morphology, with nanospheres being more uniformly distributed in cells than nanostars. Gold nanospheres were also found to be more effective in mechanobiological modulations. They significantly suppressed the migratory ability of cells under different incubation times while lowering the bulk stiffness and adhesion of cells. This in vitro study suggests the potential applications of gold nanoparticles to manage cell migration. Nano-bio-interactions appeared to impact the cytoskeletal organization of cells and consequently alter the mechanical properties of cells, which could influence the cellular functions of cells. According to the results and migratory index model, it is thought that nanoparticle-treated cells experience mechanical changes in their body, which largely reduces their migratory potentials. These findings provide a better understanding of nano-bio-interaction in terms of cell mechanics and highlight the importance of mechanobiological responses in designing gold nanoparticles for cancer therapy.

Behavior of Citrate-Capped Ultrasmall Gold Nanoparticles on a Supported Lipid Bilayer Interface at Atomic Resolution

“…The observed differences in systematic structural changes between the DOPC SLB and DPPC SLB during particle adsorption could potentially explain a potential mechanism of biocompatibility/toxicity, tailoring this factor could enhance their functionality. The successful incorporation into the fluid phasewithout significant membrane deformation (aside from localized lipid ordering on AuNP surface)may indicate potential biocompatibility (however, the overall unlocalized destabilizing effects are still present as well as the localized welling effects); whereas the temporary buckling effects observed in the gel phase, along with the slower self-assembly rate (lipid velocity) causing significant overall structural changes to persist within the membrane causing the membrane to distort past its natural limit, could give rise to effects such as membrane leakage, cell lysis, and intracellular water/ion penetration. , Tailoring the extent of particle adsorption via modulation of ligand, specificity to lipid domains phase, or phase-specific proteins will also allow for modulation of lipid mechanical features, cell function, and morphology as well as particle location and behavior . MD simuluations revealed that the AuNP is still able to destabilize the DPPC membrane but has alternative mechanisms dependent on the lipid phase (see Figure ).…”

Comparing the bone regeneration potential between a trabecular bone and a porous scaffold through osteoblast migration and differentiation: A multiscale approach

“…Kashani and Packirisamy 11 introduced a validated FE model on cell migration and showed that cell migration can be represented by the strain energy density (SED). Later, they also utilized this SED model in an in vitro study to control migration of cells 12 . Therefore, SED in cell interface can be employed as a determining parameter for cell migration, particularly when the adhesion force remains constant 11 …”

“…Later, they also utilized this SED model in an in vitro study to control migration of cells. 12 Therefore, SED in cell interface can be employed as a determining parameter for cell migration, particularly when the adhesion force remains constant. 11 Trabecular bone is the porous and sponge-like structure inside the bone, which is accompanied by bone marrow.…”

“…38 From the literature, it is clear that osteogenic differentiation and cell migration both are important factors for bone regeneration. 4,39,40 But, no previous computational work has analysed both FSS (controlling parameter for osteogenic differentiation 21,22 ) and SED (controlling parameter for cell migration 11,12 ) as two critical factors for bone regeneration in a single study. Additionally, to the best of our knowledge, there has been no comparison between a trabecular bone and a porous scaffold while considering FSS and SED as response parameters in any previous study.…”

Fabrication of copper sulfide nanoparticles from boswellia ovalifoliolata leaf extract and their potential application of environmental pollutants removal, antibacterial activity and antioxidant activity

“…Nanoparticles can be used for drug designing, drug formulation, etc., mainly nanoparticles can be used for the treatment of cancer. Using nanoparticles we can do the photo-thermal ablation studies to study the cancer cells in our human body [14].…”