Gold Nano-Popcorn Attached SWCNT Hybrid Nanomaterial for Targeted Diagnosis and Photothermal Therapy of Human Breast Cancer Cells

Abstract

Breast cancer presents greatest challenge in health care in today’s world. The key to ultimately successful treatment of breast cancer disease is an early and accurate diagnosis. Current breast cancer treatments are often associated with severe side effects. Driven by the need, we report the design of novel hybrid nanomaterial using gold nano popcorn-attached single wall carbon nanotube for targeted diagnosis and selective photothermal treatment. Targeted SK-BR-3 human breast cancer cell sensing have been performed in 10 cancer cells/mL level, using surface enhanced Raman scattering of single walls carbon nanotube’s D and G bands. Our data show that S6 aptamer attached hybrid nanomaterial based SERS assay is highly sensitive to targeted human breast cancer SK-BR-3 cell line and it will be able to distinguish it from other non targeted MDA-MB breast cancer cell line and HaCaT normal skin cell line. Our results also show that 10 minutes of photothermal therapy treatment by 1.5 W/cm2 power, 785 nm laser is enough to kill cancer cells very effectively using S6 aptamer attached hybrid nanomaterials. Possible mechanisms for targeted sensing and operating principle for highly efficient photothermal therapy have been discussed. Our experimental results reported here open up a new possibility for using aptamers modified hybrid nanomaterial for reliable diagnosis and targeted therapy of cancer cell lines quickly.

SERS Tags: Novel Optical Nanoprobes for Bioanalysis

“…Encouraged by this work, the group designed a novel hybrid nanomaterial using popcorn-shaped, gold nanoparticle-attached, carbon nanotubes for diagnosis and selective photothermal treatment. 280 For SERS detection purposes, they directly incorporated Raman spectroscopic properties (D-band at1340 cm…”

Application of nanoparticles in cancer detection by Raman scattering based techniques

“…They compared this selective binding in MDA-MB as an HER2-negative breast cancer cell line. Due to the formation of aggregates in the presence of SKBR3 cells, it formed several hot spots, which provided significant enhancement of Raman signal intensity from SWCNTs by three orders of magnitude through electromagnetic field enhancements [39,44]. SERS nano-tags with metallic NPs in the form of nano rods, hollow nanostructures, and nano stars because of having an active hot spot could give out higher sensitivity that is useful for in vivo applications.…”

“…They compared this selective binding in MDA-MB as HER2-negative breast cancer cell line. Due to the formation of aggregates in the presence of SKBR3 cells, it formed several hot spots, which provided a significant enhancement of the Raman signal intensity from SWCNTs by three orders of magnitude through electromagnetic field enhancements (Figure 5) [39]. Other criteria can be used to detect breast cancer, In one paper, Matousek and Stone [80] showed the ability of Raman system to detect a calcified inclusion within a thick layer of tissue, with potential applications of breast cancer diagnosis (Figure 6).…”

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10.1080/20022727.2017.1373551-F0005Figure 5.(a) Plot showing SERS. (b) Schematic representation shows the synthesis protocol for the formation of GNPOP attached SWCNTs [39].

10.1080/20022727.2017.1373551-F0006Figure 6.Cancer cell targeting and spectroscopic detection by using antibody-conjugated SERS nanoparticles.

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“…(b) Schematic representation shows the synthesis protocol for the formation of GNPOP attached SWCNTs [39]. …”