Gold NanoBipyramids Performing as Highly Sensitive Dual-Modal Optical Immunosensors

Campu, Andreea; Lerouge, Frédéric; Château, Denis; Chaput, Frédéric; Baldeck, Patrice L.; Parola, Stéphane; Maniu, Dana; Craciun, Ana-Maria; Vulpoi, Adriana; Aştilean, Simion; Focșan, Monica

doi:10.1021/acs.analchem.8b01689

Cited by 48 publications

(41 citation statements)

References 41 publications

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“…In addition, Au NBPs have unique optical properties compared with other gold nanoparticles (such as gold nanorods, gold nanostars, and gold nanocages). Theoretical calculations show that the photothermal effect of Au NBPs is several times stronger than that of gold nanorods, 25,26 and the sharpness of the two ends of Au NBPs is directly proportional to the local plasmon resonance effect in the near-infrared range. 27 Release of Minocycline in vitro Figure 3A shows the cumulative amounts of MINO released from the hydrogels in PBS buffer (pH= 7.4) at 37 °C.…”

Section: Dovepressmentioning

confidence: 99%

<p>Hybrid Hydrogels for Synergistic Periodontal Antibacterial Treatment with Sustained Drug Release and NIR-Responsive Photothermal Effect</p>

Lin¹,

He²,

Liu³

et al. 2020

IJN

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Background: Periodontal pathogenic bacteria promote the destruction of periodontal tissues and cause loosening and loss of teeth in adults. However, complete removal of periodontal pathogenic bacteria, at both the bottom of the periodontal pocket and the root bifurcation area, remains challenging. In this work, we explored a synergistic antibiotic and photothermal treatment, which is considered an alternative strategy for highly efficient periodontal antibacterial therapy. Methods: Mesoporous silica (MSNs) on the surface of Au nanobipyramids (Au NBPs) were designed to achieve the sustained release of the drug and photothermal antibacterials. The mesoporous silica-coated Au NBPs (Au NBPs@SiO 2) were mixed with gelatin methacrylate (GelMA-Au NBPs@SiO 2). Au NBPs@SiO 2 and GelMA-Au NBPs@SiO 2 hybrid hydrogels were characterized, and the drug content and photothermal properties in terms of the release profile, bacterial inhibition, and cell growth were investigated. Results: The GelMA-Au NBPs@SiO 2 hybrid hydrogels showed controllable minocycline delivery, and the drug release rates increased under 808 nm near-infrared (NIR) light irradiation. The hydrogels also exhibited excellent antibacterial properties, and the antibacterial efficacy of the antibiotic and photothermal treatment was as high as 90% and 66.7% against Porphyromonas gingivalis (P. gingivalis), respectively. Moreover, regardless of NIR irradiation, cell viability was over 80% and the concentration of Au NBPs@SiO 2 in the hybrid hydrogels was as high as 100 µg/mL. Conclusion: We designed a new near-infrared light (NIR)-activated hybrid hydrogel that offers both sustained release of antibacterial drugs and photothermal treatment. Such sustained release pattern yields the potential to rapidly eliminate periodontal pathogens in the periodontal pocket, and the photothermal treatment maintains low bacterial retention after the drug treatment.

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Section: Dovepressmentioning

confidence: 99%

<p>Hybrid Hydrogels for Synergistic Periodontal Antibacterial Treatment with Sustained Drug Release and NIR-Responsive Photothermal Effect</p>

Lin¹,

He²,

Liu³

et al. 2020

IJN

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“…Moreover, the transversal band does not show any modification in position, while for the longitudinal LSPR response, red-shifts from 3 to 10 nm were noted in the presence of p-ATP molecules, indicating that they were successfully grafted on all GNRs systems. In fact, this red-shift of the longitudinal LSPR is due to the change of the surface refractive index, thus proving the capture of the p-ATP analyte at the exposed ends of the nanoparticles [ 16 ], which are more likely to attach molecules since the CTAB bilayer tends to bind more strongly to the {1 0 0} lateral faces and less to the end {1 1 1} and {1 1 0} crystal planes of the GNRs [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…Recently, our group has succeeded in fabricating a multifunctional sensing platform by integrating an LSPR transducer with SERS activity on a flexible three-dimensional (3D) Au nanocups substrate [ 15 ]. The same group successfully demonstrated the feasibility of gold bipyramidal-shaped nanoparticles (AuBPs) to be used as active plasmonic nanoplatforms for the detection of the biotin-streptavidin interaction in aqueous solution via both LSPR and SERS [ 16 ]. However, compared to other substrates, paper can yield better SERS enhancement due to its flexible three-dimensional (3D) matrix, onto which NPs can be easily adsorbed and stabilized [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Designing Efficient Low-Cost Paper-Based Sensing Plasmonic Nanoplatforms

Susu

Campu

Craciun

et al. 2018

Sensors

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Paper-based platforms can be a promising choice as portable sensors due to their low-cost and facile fabrication, ease of use, high sensitivity, specificity and flexibility. By combining the qualities of these 3D platforms with the optical properties of gold nanoparticles, it is possible to create efficient nanodevices with desired biosensing functionalities. In this work, we propose a new plasmonic paper-based dual localized surface plasmon resonance–surface-enhanced Raman scattering (LSPR-SERS) nanoplatform with improved detection abilities in terms of high sensitivity, uniformity and reproducibility. Specifically, colloidal gold nanorods (GNRs) with a well-controlled plasmonic response were firstly synthesized and validated as efficient dual LSPR-SERS nanosensors in solution using the p-aminothiophenol (p-ATP) analyte. GNRs were then efficiently immobilized onto the paper via the immersion approach, thus obtaining plasmonic nanoplatforms with a modulated LSPR response. The successful deposition of the nanoparticles onto the cellulose fibers was confirmed by LSPR measurements, which demonstrate the preserved plasmonic response after immobilization, as well as by dark-field microscopy and scanning electron microscopy investigations, which confirm their uniform distribution. Finally, a limit of detection for p-ATP as low as 10−12 M has been achieved by our developed SERS-based paper nanoplatform, proving that our optimized plasmonic paper-based biosensing design could be further considered as an excellent candidate for miniaturized biomedical applications.

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“…Gold bipyramids (AuBPs), as attractive anisotropic nanotransducers, respond well to this demand, offering the possibility to be synthesized in such way that will induce a fine tailoring of the longitudinal LSPR band over the entire electromagnetic spectrum. Our previous study demonstrates the feasibility of these diamond-like shaped nanostructures to be implemented as highly sensitive LSPR immunosensors in aqueous solution (Campu et al, 2018). Briefly, the ability of different aspect ratio AuBPs to efficiently detect target molecules was proven for antigen-antibody interactions both simple “proof-of-concept” reactions, such as the biotin-streptavidin recognition, and more complex real-sample reactions like anti-human IgG–human IgG binding.…”

Section: Introductionmentioning

confidence: 95%

Multimodal Biosensing on Paper-Based Platform Fabricated by Plasmonic Calligraphy Using Gold Nanobypiramids Ink

et al. 2019

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In this work, we design new plasmonic paper-based nanoplatforms with interesting capabilities in terms of sensitivity, efficiency, and reproducibility for promoting multimodal biodetection via Localized Surface Plasmon Resonance (LSPR), Surface Enhanced Raman Spectroscopy (SERS), and Metal Enhanced Fluorescence (MEF). To succeed, we exploit the unique optical properties of gold nanobipyramids (AuBPs) deposited onto the cellulose fibers via plasmonic calligraphy using a commercial pen. The first step of the biosensing protocol was to precisely graft the previously chemically-formed p-aminothiophenol@Biotin system, as active recognition element for target streptavidin detection, onto the plasmonic nanoplatform. The specific capture of the target protein was successfully demonstrated using three complementary sensing techniques. As a result, while the LSPR based sensing capabilities of the nanoplatform were proved by successive 13–18 nm red shifts of the longitudinal LSPR associated with the change of the surface RI after each step. By employing the ultrasensitive SERS technique, we were able to indirectly confirm the molecular identification of the biotin-streptavidin interaction due to the protein fingerprint bands assigned to amide I, amide III, and Trp vibrations. Additionally, the formed biotin-streptavidin complex acted as a spacer to ensure an optimal distance between the AuBP surface and the Alexa 680 fluorophore for achieving a 2-fold fluorescence emission enhancement of streptavidin@Alexa 680 on the biotinylated nanoplatform compared to the same complex on bare paper (near the plasmonic lines), implementing thus a novel MEF sensing nanoplatform. Finally, by integrating multiple LSPR, SERS, and MEF nanosensors with multiplex capability into a single flexible and portable plasmonic nanoplatform, we could overcome important limits in the field of portable point-of-care diagnostics.

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Gold NanoBipyramids Performing as Highly Sensitive Dual-Modal Optical Immunosensors

Cited by 48 publications

References 41 publications

<p>Hybrid Hydrogels for Synergistic Periodontal Antibacterial Treatment with Sustained Drug Release and NIR-Responsive Photothermal Effect</p>

<p>Hybrid Hydrogels for Synergistic Periodontal Antibacterial Treatment with Sustained Drug Release and NIR-Responsive Photothermal Effect</p>

Designing Efficient Low-Cost Paper-Based Sensing Plasmonic Nanoplatforms

Multimodal Biosensing on Paper-Based Platform Fabricated by Plasmonic Calligraphy Using Gold Nanobypiramids Ink

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