Gold Nanocluster-Based Nanozyme for High-Throughput Colorimetric Detection of SARS-CoV-2

Abstract

There is an urgent need for efficient and convenient methods to detect SARS-CoV-2 as the global pandemic continues. Currently, detection of COVID-19 relies primarily on reverse transcription PCR (RT-PCR), a laboratory-based method that is time-consuming. To tackle this challenge, the study proposes an immediate high-throughput detection platform for SARS-CoV-2. The platform uses a gold nanoenzymatic colorimetric assay that focuses on the specific detection of 3CLpro in SARS-CoV-2. The platform has been extensively characterized for stability, sensitivity, detection range, and response time. The colorimetric detection method not only provides easy-to-read results but also allows color readings to be taken using a smartphone, with accuracy comparable to that of large instruments such as a microplate reader. The innovation of this technology lies in the ability to achieve instant high-throughput detection, which makes rapid detection of SARS-CoV-2 feasible. The proposed detection platform has a wide range of potential applications, particularly in the field of virus detection, providing an efficient and reliable solution for managing current pandemics and future disease prevention and control.

Silver Nanoparticle-Immobilized Schiff-Base Macrocycles as Nanozymes with Peroxidase Mimic Activity for Antibacterial Films

“…Combining the advantages of nanomaterials and natural enzymes, artificial nanozymes have emerged as promising candidates in combating bacterial infections. − Unlike natural enzymes, nanozymes are suitable for large-scale production, have adjustable enzyme-like activity, low production costs, and high environmental stability. , So far, numerous nanozymes have been reported to exhibit good antimicrobial properties, including metal oxides and metal sulfides, , precious metals, , quantum dots, , MOFs, , metal composites, , and single atom materials. − However, complex design, relatively low bactericidal activity, and potential toxicity to normal cells or tissues still hinder their practical use. For example, Wang et al .…”