Gold Nanohole Array Substrates as Immunobiosensors

Abstract

A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.

Surface Plasmon Resonance Biosensors for Highly Sensitive Detection of Small Biomolecules

“…They are of interest for use in biosensors as they have a lower functionalisation area giving rise to increased sensitivity, a simple optical set-up with transmission measurement of well-defined spectral features and they allow for optical field concentration giving interesting enhancement effects. As a precursor to their use in immunobiosensors, immunochemical interactions were studied using a gold nanohole array where the signal response is expressed as a movement in the plasmon transmission peak of the array which can be simply measured using a UV-visible spectrophotometer (Sharpe et al, 2008). In this case, monoclonal antibody binding to immobilised cortisol could be clearly detected as could enhancement with gold-nanoparticle labelled secondary antibody (Fig.…”

“…The system could also be regenerated and had low non-specific binding from the gold nanoparticles (Sharpe et al, 2008). …”

Use of thermally annealed multilayer gold nanoparticle films in combination analysis of localized surface plasmon resonance sensing and MALDI mass spectrometry

“…This LSPR wavelength shift, which is based on the change in the refractive index, can be examined by using UV-vis transmission or reflection spectroscopy. Several types of LSPR sensing platforms have been reported such as substrates on which immobilized NPs from a colloidal solution, [10][11][12][13][14][15][16][17][18] lithographically fabricated triangular-nanocrystal arrays, [19][20][21][22][23][24][25][26][27][28] nanohole arrays produced by ion-beam lithography, [29][30][31][32] and transparent substrates on which metal island films are fabricated by vapor deposition. [33][34][35][36][37][38][39] When LSPR sensing is conducted in transmission mode, it is known as transmission localized surface plasmon resonance (T-LSPR) sensing.…”

Desarrollo de superficies nanoestructuradas para biosensores ópticos y sensores biomiméticos

“…Tal es la variedad de técnicas que desde hace más de una década se han desarrollado multitud de dispositivos plasmónicos basados en redes de agujeros submicrométricos realizados en películas metálicas, principalmente de oro y plata. 51,[95][96][97][98][147][148][149][150][151][152][153] Cada uno de los métodos litográficos enumerados tiene sus ventajas e inconvenientes, y su elección está condicionada por factores tales como su empleo para investigación o prototipado -para lo cual EBL y FIB son adecuadas-o su potencial uso para producción en masa -para lo que las técnicas de nanoimpresión UV, 99 Otra aplicación de la técnica EBL con relación directa con esta Tesis es la capacidad para definir nanopilares mediante el empleo de resinas negativas. En los últimos años se han publicado numerosos trabajos basados en redes de nanopilares aplicados a la biodetección empleando diferentes técnicas y materiales, [154][155][156][157][158] y las técnicas litográficas mencionadas anteriormente ofrecen la posibilidad de nanoestructurar resinas poliméricas y crear, en un número reducido de etapas, nanocolumnas con alta resolución y relación de aspecto.…”

“…A título comparativo, valores del mismo orden de S B han sido publicados con anterioridad para redes de nanoagujeros de 500 nm de periodo fabricados en plata 204 (494 nm RIU -1 ) y de 520 nm de periodo realizados en oro 149 (393 nm RIU -1 ).…”