Gold nanolabels and enzymatic recycling dual amplification-based electrochemical immunosensor for the highly sensitive detection of carcinoembryonic antigen

Zhang, YuYong; Xiang, Yun; Yuan, Ruo; Qian, Xiaoqing; Zhang, Haixia; Chen, Ying; Su, Jiao; Xu, Jin

doi:10.1007/s11426-011-4373-0

Cited by 17 publications

(6 citation statements)

References 38 publications

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“…The sensitivity of the proposed immunosensing approach was compared to some previous electrochemical CEA biosensors based on amplification-free, − and amplification detection approaches. − As depicted in Table , our proposed biosensor exhibited excellent performance regarding its detection limit and analytical range, which either exceeded or matched the performance of the mentioned examples. It is particularly advantageous that our approach does not require labeling or successive steps of isothermal enzymatic signal amplification, thus reducing the potential for errors and simplifying the detection process.…”

Section: Resultsmentioning

confidence: 93%

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Bahri,

Fredj,

Qin

et al. 2024

ACS Appl. Nano Mater.

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Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the everincreasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL −1 , the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL −1 , surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF's large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.

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Section: Resultsmentioning

confidence: 93%

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Bahri,

Fredj,

Qin

et al. 2024

ACS Appl. Nano Mater.

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“…The coreactant-free ECL immunosensor is constructed with a sandwich-type immunoassay procedure by immobilizing Ab 1 onto the bare GCE surface with ABA as the linker (Scheme B). , First, ABA is covalently immobilized onto the bare GCE surface to form GCE|ABA via electrochemical oxidation . Then, the carboxyl groups of ABA are activated with EDC and NHS (20 μL 100 mg/mL) for 30 min and incubated with PBS (10 mmol/L pH 7.4) containing 10 μg/mL Ab 1 for 3 h to obtain GCE|ABA-Ab 1 .…”

Section: Methodsmentioning

confidence: 99%

“…42,43 First, ABA is covalently immobilized onto the bare GCE surface to form GCE|ABA via electrochemical oxidation. 44 Then, the carboxyl groups of ABA are activated with EDC and NHS (20 μL 100 mg/mL) for 30 min and incubated with PBS (10 mmol/L pH 7.4) containing 10 μg/ mL Ab 1 for 3 h to obtain GCE|ABA-Ab 1 . After the activated carboxyl is blocked by 1% BSA for 30 min, 20 μL of CEA of different concentrations is incubated with GCE|ABA-Ab 1 for 2 h at 37 °C to obtain GCE|ABA-Ab 1 ⟨CEA.…”

Section: Introductionmentioning

confidence: 99%

Coreactant-free and Near-Infrared Electrochemiluminescence Immunoassay with n-Type Au Nanocrystals as Luminophores

Liu²,

Zeng³

et al. 2022

Anal. Chem.

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The electrochemiluminescence (ECL) bioassay is prominently carried out with the involvement of the coreactant. To remove the detrimental effects of the coreactant on the ECL of luminophores, herein, a promising ECL immunoassay strategy with biocompatible nanoparticles as the luminophore is proposed, which involves directly and electrochemically oxidizing the luminophore methionine-capped Au (Met@Au) nanocrystals (NCs) without the participation of any coreactant. Met@Au NCs are a kind of n-type nanoparticles, and they can be electrochemically injected with valence band (VB) holes around +0.80 and +1.10 V (vs Ag/AgCl). The electrochemically injected exogenous VB hole can recombine with the endogenous conduction band electron of Met@Au NCs and eventually bring out two coreactant-free and near-infrared ECL processes around 0.80 V (ECL-1) and 1.10 V (ECL-2). The intensity of coreactant-free ECL is primarily determined by the electrochemical oxidation-induced hole-injection process. ECL-2 is considerably stronger than ECL-1 and can be exploited for determining the carcinoembryonic antigen (CEA) in a sandwich immunoassay procedure with a linear range from 0.1 to 50 pg/mL as well as a limit of detection of 0.03 pg/mL (S/N = 3).

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“…The detection limit of CEA was 2 Â 10 À3 ng mL À1 . 17 Lin et al have summarized many chemiluminescence assays to determine different tumor markers. 14 Our group has also described a new electrochemical immunosensor electrode using the atom transfer radical polymerization (ATRP) method to prepare P(PEGMA) polymer brushes for the immobilization of anti-CEA.…”

Section: Introductionmentioning

confidence: 99%

A chitosan-Au-hyperbranched polyester nanoparticles-based antifouling immunosensor for sensitive detection of carcinoembryonic antigen

Sun

Qian

Parmar

et al. 2014

Analyst

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Analysts are always interested in finding new functional nanomaterials and devices with good properties for electrochemical sensor applications. In this paper, hyperbranched polyester nanoparticles with carboxylic acid functional groups (HBPE-CA NPs) were synthesized and combined with chitosan wrapped around Au nanoparticles (CS-Au NPs) to prepare a novel and sensitive electrochemical immunosensor by adsorption of carcinoembryonic antibody (anti-CEA) on the (HBPE-CA)/CS-Au NPs modified glass carbon electrode (GCE). Under the optimized conditions, the proposed immunosensor displayed a good amperometric response to carcinoembryonic antigen (CEA). Moreover, based on the antibiofouling properties, the immunosensor could be used for the direct detection of CEA in whole blood, and exhibited a wide detection range (1-10(7) fg mL(-1)), and a low detection limit of 0.251 fg mL(-1) (signal/noise = 3). Control experiments were also carried out by using ascorbic acid (AA), uric acid (UA), human immunoglobulin G (IgG), BSA and glucose in the absence of CEA. The good stability and repeatability of this immunosensor were also proven. Importantly, the results of the detection of clinical whole blood specimens with the proposed immunosensor showed good consistency with the data determined by enzyme-linked immunosorbent assay (ELISA) in serum samples. Furthermore, the developed immunosensor could provide a promising immunoassay strategy for clinical applications, since the values we measured in whole blood directly are likely closer to the real values.

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Gold nanolabels and enzymatic recycling dual amplification-based electrochemical immunosensor for the highly sensitive detection of carcinoembryonic antigen

Cited by 17 publications

References 38 publications

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Coreactant-free and Near-Infrared Electrochemiluminescence Immunoassay with n-Type Au Nanocrystals as Luminophores

A chitosan-Au-hyperbranched polyester nanoparticles-based antifouling immunosensor for sensitive detection of carcinoembryonic antigen

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