Gold nanoparticle&amp;ndash;choline complexes can block nicotinic acetylcholine receptors

Chin, Chur; Kim, In Kyeom; Lim, Dong Yoon; Kim, Ki Suk; Lee, Hyang Ae; Kim, Eun Joo

doi:10.2147/ijn.s10466

Cited by 10 publications

(8 citation statements)

References 9 publications

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“…Voltage-gated ion channels are generally composed of two main parts, the pore-forming transmembrane domains (including voltage-sensing modules), and cytoplasmic loops [50] . Recent studies showed that the pores of ion channels such as hERG and nicotinic acetylcholine receptor (nAChRs) can be directly clogged with ultra-small AuNPs (∼1.4-nm diameter) and impede the movement of ions through the channel pore [51] , [52] . However, the estimated pore sizes of Na + and K + channels are either below or approximately 10 Å in diameter [53] , [54] ; therefore, the AuNPs used in our study were too large to physically internalize within the channels' pore.…”

Section: Discussionmentioning

confidence: 99%

Intracellular Gold Nanoparticles Increase Neuronal Excitability and Aggravate Seizure Activity in the Mouse Brain

et al. 2014

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Due to their inert property, gold nanoparticles (AuNPs) have drawn considerable attention; their biological application has recently expanded to include nanomedicine and neuroscience. However, the effect of AuNPs on the bioelectrical properties of a single neuron remains unknown. Here we present the effect of AuNPs on a single neuron under physiological and pathological conditions in vitro. AuNPs were intracellularly applied to hippocampal CA1 neurons from the mouse brain. The electrophysiological property of CA1 neurons treated with 5- or 40-nm AuNPs was assessed using the whole-cell patch-clamp technique. Intracellular application of AuNPs increased both the number of action potentials (APs) and input resistance. The threshold and duration of APs and the after hyperpolarization (AHP) were decreased by the intracellular AuNPs. In addition, intracellular AuNPs elicited paroxysmal depolarizing shift-like firing patterns during sustained repetitive firings (SRF) induced by prolonged depolarization (10 sec). Furthermore, low Mg2+-induced epileptiform activity was aggravated by the intracellular AuNPs. In this study, we demonstrated that intracellular AuNPs alter the intrinsic properties of neurons toward increasing their excitability, and may have deleterious effects on neurons under pathological conditions, such as seizure. These results provide some considerable direction on application of AuNPs into central nervous system (CNS)

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Section: Discussionmentioning

confidence: 99%

Intracellular Gold Nanoparticles Increase Neuronal Excitability and Aggravate Seizure Activity in the Mouse Brain

et al. 2014

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“…Nanoparticle-cell interactions often lead to accidental toxic effects such as blocking of ion channels [58,59], disturbance of the membrane potential induced by nanoporation [60,61], or inhibition of signaling pathways or other essential cell functions such as nuclear chromatin decondensation [62].…”

Section: Cell-nanoparticle Interactions In Generalmentioning

confidence: 99%

Challenges in drug delivery to the brain: Nature is against us

Krol

2012

Journal of Controlled Release

134

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“…In the case of Fe 3 O 4 , the properties of small size and charge may interfere with cell activity. It was reported that nanoparticles can block the receptor [ 57 ]. A small amount of the osteoconduction obtained in Fe 3 O 4 NPs might be due to the aggregation effect.…”

Section: Resultsmentioning

confidence: 99%

Supermagnetic Sugarcane Bagasse Hydrochar for Enhanced Osteoconduction in Human Adipose Tissue-Derived Mesenchymal Stem Cells

et al. 2020

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Hydrothermally carbonized sugarcane bagasse (SCB) has exceptional surface properties. Looking at the huge amount of SCB produced, its biocompatible nature, cheap-cost for carbonization, and its easy functionalization can give impeccable nano-biomaterials for tissue engineering applications. Herein, sugarcane bagasse was converted into hydrochar (SCB-H) by hydrothermal carbonation. The SCB-H produced was further modified with iron oxide (Fe3O4) nanoparticles (denoted as SCB-H@Fe3O4). Facile synthesized nano-bio-composites were characterized by SEM, HR-TEM, XRD, FT-IR, XPS, TGA, and VSM analysis. Bare Fe3O4 nanoparticles (NPs), SCB-H, and SCB-H@Fe3O4 were tested for cytocompatibility and osteoconduction enhancement of human adipose tissue-derived mesenchymal stem cells (hADMSCs). The results confirmed the cytocompatible and nontoxic nature of SCB-H@Fe3O4. SCB-H did not show enhancement in osteoconduction, whilst on the other hand, Fe3O4 NPs exhibited a 0.5-fold increase in the osteoconduction of hADMSCs. However, SCB-H@Fe3O4 demonstrated an excellent enhancement in osteoconduction of a 3-fold increase over the control, and a 2.5-fold increase over the bare Fe3O4 NPs. Correspondingly, the expression patterns assessment of osteoconduction marker genes (ALP, OCN, and RUNX2) confirmed the osteoconductive enhancement by SCB-H@Fe3O4. In the proposed mechanism, the surface of SCB-H@Fe3O4 might provide a unique topology, and anchoring to receptors of hADMSCs leads to accelerated osteogenesis. In conclusion, agriculture waste-derived sustainable materials like “SCB-H@Fe3O44” can be potentially applied in highly valued medicinal applications of stem cell differentiation.

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Gold nanoparticle–choline complexes can block nicotinic acetylcholine receptors

Cited by 10 publications

References 9 publications

Intracellular Gold Nanoparticles Increase Neuronal Excitability and Aggravate Seizure Activity in the Mouse Brain

Intracellular Gold Nanoparticles Increase Neuronal Excitability and Aggravate Seizure Activity in the Mouse Brain

Challenges in drug delivery to the brain: Nature is against us

Supermagnetic Sugarcane Bagasse Hydrochar for Enhanced Osteoconduction in Human Adipose Tissue-Derived Mesenchymal Stem Cells

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