Gold nanoparticle-assisted polymerase chain reaction: effects of surface ligands, nanoparticle shape and material

Vanzha, Ekaterina; Pylaev, Timofey; Khanadeev, Vitaly A.; Konnova, Svetlana S.; Fedorova, V A; Khlebtsov, Nikolai G.

doi:10.1039/c6ra20472d

Cited by 32 publications

(16 citation statements)

References 45 publications

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“…These PCR results confirm that the immobilized oligonucleotides on the gold nanoparticle surfaces maintain their accessibility to perform PCR contributing also to reducing the presence of unspecific bands. This effect was also described previously for the detection of the nitrogen fixation gene in Azospirillum bacteria and for other DNAs [16,17,18]. Taken together, here we demonstrated that an oligonucleotide anchored to the Au@SiO2–COOH is accessible for further recognition by methylated DNA of RPRM .…”

Section: Resultssupporting

confidence: 88%

Gold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

et al. 2019

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Reprimo (RPRM) is a tumor suppressor gene involved in the development of gastric cancer. Hypermethylation of the RPRM promoter region has been found in tumor tissue and plasma samples from patients with gastric cancer. These findings suggest that circulating methylated DNA of RPRM could be a candidate for a noninvasive detection of gastric cancer. We designed a nanosystem based on the functionalization of silica coated gold nanoparticles with oligonucleotides that recognize a specific DNA fragment of the RPRM promoter region. The functionality of the oligonucleotide on the surface of the nanoparticle was confirmed by polymerase chain reaction (PCR). The nanoparticles were incubated with a synthetic DNA fragment of methylated DNA of RPRM and changes in the size distribution after hybridization were evaluated by dynamic light scattering (DLS). A difference in the size distribution of nanoparticles hybridized with genomic DNA from the KATO III gastric cancer cell line was observed when was compared with DNA from the GES-1 normal cell line. These results showed that this nanosystem may be a useful tool for the specific and sensitive detection of methylated DNA of RPRM in patients at risk of developing gastric cancer.

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Section: Resultssupporting

confidence: 88%

Gold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

et al. 2019

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“…It exploits the light-to-heat conversion mediated by plasmonic substrates [26][27][28][29] . This concept has been adopted for PCR thermocycling, in the form of a planar plasmonic substrate in a fluidic device 20,30,31 or metallic nanoparticles in suspension 22,32,33 . Both methods have shown fast nucleic acid ampli-fication, but challenges remain: nucleic acids after amplification are difficult to detect because plasmonic substrates would interfere with fluorescence reporters 34,35 .…”

mentioning

confidence: 99%

Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

et al. 2020

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“…[ 11,39,40 ] In the meantime, some other characters of nanoparticles may also influence the PCR amplification, such as surface ligands, nanoparticle shape, and material. [ 41 ] Therefore, the true processes that occur in the nano‐localized environment during NA amplification still need to be explored, which is critical to optimize the nano‐localized NA amplification.…”

Section: Discussionmentioning

confidence: 99%

Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

You

Jia

et al. 2021

Small Methods

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Fast nucleic acid (NA) amplification has found widespread biomedical applications, where high thermocycling rate is the key. The plasmon‐driven nano‐localized thermocycling around the gold nanorods (AuNRs) is a promising alternative, as the significantly reduced reaction volume enables a rapid temperature response. However, quantifying and adjusting the nano‐localized temperature field remains challenging for now. Herein, a simple method is developed to quantify and adjust the nano‐localized temperature field around AuNRs by combining experimental measurement and numerical simulation. An indirect method to measure the surface temperature of AuNRs is first developed by utilizing the temperature‐dependent stability of Authiol bond. Meanwhile, the relationship of AuNRs′ surface temperature with the AuNRs concentration and laser intensity, is also studied. In combination with thermal diffusion simulation, the nano‐localized temperature field under the laser irradiation is obtained. The results show that the restricted reaction volume (≈aL level) enables ultrafast thermocycling rate (>104 °C s−1). At last, a duplex‐specific nuclease (DSN)‐mediated isothermal amplification is successfully demonstrated within the nano‐localized temperature field. It is envisioned that the developed method for quantifying and adjusting the nano‐localized temperature field around AuNRs is adaptive for various noble metal nanostructures and will facilitate the development of the biochemical reaction in the nano‐localized environment.

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Gold nanoparticle-assisted polymerase chain reaction: effects of surface ligands, nanoparticle shape and material

Abstract: The concentration, shape, material, and surface functionalization effects of gold nanoparticles on PCR outcome have been studied with two PCR gene diagnostic models.

Cited by 32 publications

References 45 publications

Gold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

Gold@Silica Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

Quantifying and Adjusting Plasmon‐Driven Nano‐Localized Temperature Field around Gold Nanorods for Nucleic Acids Amplification

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