Gold nanoparticle-based aptasensors: A promising perspective for early-stage detection of cancer biomarkers

Atapour, Amir; Khajehzadeh, Hossein; Shafie, Mostafa; Abbasi, Milad; Mosleh-Shirazi, Sareh; Kasaee, Seyed Reza; Amani, Ali Mohammad

doi:10.1016/j.mtcomm.2022.103181

Cited by 37 publications

(12 citation statements)

References 201 publications

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“…For the FcSH/aptamer/MWCNT/AuNP electrode the R ct increases to 2213 Ω, implying that the SAM of FcSH forms a thinner diffusion layer as compared to the SAM of MCH diffusion layer. Based on the EIS results, sensitive amperometric detection could be achieved by simply switching the MCH for FcSH during the co-immobilization of the aptamer probe, while for the impedimetric detection MCH improves the surface chemistry [ 54 ]. In regard to double layer capacitance, the C dl of the bare electrode raised from 7.91 × 10 −5 F to 1.01 × 10 −4 F for aptamer immobilized bare electrode.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

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Prostate cancer is one of the most frequently diagnosed male malignancies and can be detected by prostate-specific antigen (PSA) as a biomarker. To detect PSA, several studies have proposed using antibodies, which are not economical and require a long reaction time. In this study, we propose to use self-assembled thiolated single-strand DNA on electrodes functionalized by multi-walled carbon nanotubes (MWCNT) modified with gold nanoparticles (AuNPs) to realize a low-cost label-free electrochemical biosensor. In this regard, the PSA aptamer was immobilized via electrostatic adsorption on the surface of a screen-printed MWCNT/AuNPs electrode. The immobilization process was enhanced due to the presence of Au nanoparticles on the surface of the electrode. Surface characterization of the electrode at different stages of modification was performed by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) and contact angle for surface tension properties. The results showed an increase in surface roughness due to the absorbance of the aptamer on the electrode surfaces. The developed sensor has an extended linear range of 1–100 ng/mL, and a very low limit of detection down to 1 pg/mL. In addition, the reaction has a binding time of only five minutes on the developed electrodes. Investigations of the biosensor selectivity against several substances revealed an efficient selectivity for PSA detection. With this approach, low-cost biosensors with high sensitivity can be realized which have a wide linearity range and a low limit of detection, which are necessary for the early detection of prostate cancer.

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Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

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“…Aptamer‐based biosensors are a promising alternative to traditional detection methods due to their high specificity, sensitivity, and versatility [1–3] . Among these biosensors, gold nanoparticle‐based optical aptasensors have gained increasing attention in recent years due to their simplicity, cost‐effectiveness, and ease of use [4–6] . Traditionally, thiolated DNA was used to densely functionalize AuNPs, with the AuNPs serving solely as an optical label [7,8] .…”

Section: Introductionmentioning

confidence: 99%

Uncovering Adenosine‐Specific Signals: A Label‐Free Aptamer‐Based Approach with Gold Nanoparticles for Specific Detection

et al. 2023

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We present a novel colorimetric aptasensing method for specific adenosine (Ade) detection based on complementary aptamer hybridization and gold nanoparticles (AuNPs). Unlike traditional approaches, our method overcomes limitations of selectivity and specificity by incorporating an additional step involving the complementary strand of the aptamer (c‐Ade aptamer) after incubating Ade with the aptamer. In the absence of Ade, the c‐Ade aptamer hybridizes with the aptamer, causing AuNPs aggregation and a blue color change. However, in the presence of Ade, the aptamer‐bound target prevents hybridization, allowing the free c‐Ade aptamer to adsorb onto the AuNPs, resulting in a red color change. This unique color reversal distinguishes our approach from conventional label‐free methods. We optimized the detection conditions, including incubation time and salt concentration, to enhance sensitivity and reproducibility. The calibration curve exhibited a linear relationship between the colorimetric response and Ade concentration, with a limit of detection of 3.0±0.8 nM. Our method demonstrated excellent selectivity for Ade, even in the presence of structurally similar interfering compounds. Furthermore, we successfully applied our approach to detect Ade in complex biological samples with minimal sample preparation. Our colorimetric aptasensing method offers a simple, cost‐effective, and specific tool for detecting targets in various applications.

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“…Among nanomaterials, gold nanoparticles (Au NPs) have attracted remarkable attention as a result of their potential biomedical applications, such as imaging and treatment of diseases (Mosleh-Shirazi et al 2022 ). Au NPs are characteristically considered as bio-safe materials to apply as drug transport and antimicrobial materials (Selvaraj et al 2010 ; Mosleh-Shirazi et al 2021a , b ; Atapour et al 2022 ; Meisami et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Antiviral and antioxidant properties of green synthesized gold nanoparticles using Glaucium flavum leaf extract

et al. 2022

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Nowadays, nanoparticles such as gold nanoparticles (Au NPs) with specific biophysical characteristics have attracted remarkable attention as innovative options for the diagnosis and treatment of different diseases. In the present research, Au NPs were green synthesized using the Glaucium flavum leaf extract as an inexpensive and eco-friendly synthesis method. Then, the physicochemical properties were characterized by transmission electron microscopy (TEM), dynamic light scattering method (DLS), scanning electron microscopy (SEM), X-ray diffraction (XRD), Ultraviolet–visible absorption spectroscopy (UV–Vis), Zeta potential, and Fourier transform infrared (FTIR) spectroscopy. Afterwards, the antioxidant capacity was tested and antiviral activity against influenza virus was evaluated by applying TCID50 and PCR assays. The nanoparticles cytotoxicity was tested using the MTT method. The shape and size of Au nanoparticles were modulated by varying leaf concentrations with face-centered cubic (FCC) structure. At higher concentrations, long-time stable spherical nanoparticles were obtained with a mean particle size of 32 nm and low aggregation degree that could simply combine with various bioactive compounds. The outcomes exhibited effective antiviral and antioxidant activities with low cytotoxicity and acceptable biocompatibility of green synthesized Au NPs. The aim of the present study was to develop a potentially environmentally friendly nanoplatform with excellent antiviral and antioxidant functions and acceptable biocompatibility for promising biomedical applications in the future. Supplementary Information The online version contains supplementary material available at 10.1007/s13204-022-02705-1.

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Gold nanoparticle-based aptasensors: A promising perspective for early-stage detection of cancer biomarkers

Cited by 37 publications

References 201 publications

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

Uncovering Adenosine‐Specific Signals: A Label‐Free Aptamer‐Based Approach with Gold Nanoparticles for Specific Detection

Antiviral and antioxidant properties of green synthesized gold nanoparticles using Glaucium flavum leaf extract

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