Gold Nanoparticle-Based Facile Detection of Human Serum Albumin and Its Application as an INHIBIT Logic Gate

Abstract

In this work, a facile colorimetric method is developed for quantitative detection of human serum albumin (HSA) based on the antiaggregation effect of gold nanoparticles (Au NPs) in the presence of HSA. The citrate-capped Au NPs undergo a color change from red to blue when melamine is added as a cross-linker to induce the aggregation of the NPs. Such an aggregation is efficiently suppressed upon the adsorption of HSA on the particle surface. This method provides the advantages of simplicity and cost-efficiency for quantitative detection of HSA with a detection limit of ∼1.4 nM by monitoring the colorimetric changes of the Au NPs with UV-vis spectroscopy. In addition, this approach shows good selectivity for HSA over various amino acids, peptides, and proteins and is qualified for detection of HSA in a biological sample. Such an antiaggregation effect can be further extended to fabricate an INHIBIT logic gate by using HSA and melamine as inputs and the color changes of Au NPs as outputs, which may have application potentials in point-of-care medical diagnosis.

Design and optimization of a nanoprobe comprising amphiphilic chitosan colloids and Au-nanorods: Sensitive detection of human serum albumin in simulated urine

“…Furthermore, this LDL was a 10-fold improvement compared to that previously reported for a non-optimized CHC-AuNR system in the less complex environment of DI water. This pM LDL is also lower than what has been reported in recent literature for several HSA-sensing systems [32][33][34][35]. The results confirm the potential for using this sensitive nanoprobe in complex environments.…”

6-Aza-2-Thio-Thymine Stabilized Gold Nanoclusters as Photoluminescent Probe for Protein Detection

“…As a matter of fact, the gradually extensive application of few-atom metal nanomaterials has promoted a depth exploration of nanotoxicology, and, correspondingly, highlighted the significance for the biosafety evaluation to be performed. Investigations of protein-nanoparticle interactions have been reported in several studies [19][20][21][22][23]. However, as far as we know, there has been a smaller number of literatures focusing on protein determination with the help of fluorescent AuNCs [24,25].…”

Motion‐Induced Changes in Emission as an Effective Strategy for the Ratiometric Probing of Human Serum Albumin and Trypsin in Biological Fluids

“…[9][10][11][12] Of these strategies, the colorimetric assay is the most user-friendlyo ne, because it is the most cost-effective and the least tedious, requiring the minimum number of sample-preparation steps. [13][14][15][16][17][18] Most clinically approved optical methods involveB romocresol green (BCG) as the color-generating dye. [19] Although the highs ensitivity of BCG towards HSA can be beneficial for estimation purposes, it can interact with other plasma proteins within 30 so fi ncubation, which can lead to the overestimation of albumin levels.…”

“…Three different strategies have been reported for the estimation of serum albumins: 1) dye‐binding‐based optical assays; 2) antibody‐based immunochemical methods; and 3) LCMS/MS‐based proteomic techniques . Of these strategies, the colorimetric assay is the most user‐friendly one, because it is the most cost‐effective and the least tedious, requiring the minimum number of sample‐preparation steps . Most clinically approved optical methods involve Bromocresol green (BCG) as the color‐generating dye .…”