2023
DOI: 10.1039/d3qm00624g
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Gold nanoparticle-based immunochromatographic assay for the rapid detection of the SARS-CoV-2 Omicron variant

Abstract: At present, the prevalent Omicron mutant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has the characteristics of high infectivity and strong concealment, leading to more asymptomatic infections. The advantages...

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Cited by 7 publications
(3 citation statements)
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“…The preparation of AuNPs was performed following the method of reducing chloroauric acid (0.01%) with trisodium citrate (4 mL, 1%) as described by Ye et al 29 The prepared AuNP solution was wine-red with naked eye. The solution was clear and transparent, and with no precipitation and agglomeration.…”
Section: Resultsmentioning
confidence: 99%
“…The preparation of AuNPs was performed following the method of reducing chloroauric acid (0.01%) with trisodium citrate (4 mL, 1%) as described by Ye et al 29 The prepared AuNP solution was wine-red with naked eye. The solution was clear and transparent, and with no precipitation and agglomeration.…”
Section: Resultsmentioning
confidence: 99%
“…The subtype of mAb 3G10 was ascertained using a commercial isotyping kit. The affinity of 3G10 was analyzed using a calibration curve constructed by non-competitive ELISA, and the affinity constant ( K a ) was calculated according to the following formula: 36 where n is the multiple of the concentration of any two coating antigens. Ab 2 and Ab 1 are the antibody concentration at half the maximum OD value, which correspond to two coating antigens concentration mentioned above.…”
Section: Methodsmentioning
confidence: 99%
“…SP2/0 myeloma cells and spleen cells were fused with chemical PEG, as previously conducted. [16][17][18] The growth state of the cells could be observed under a microscope and 3 days after cell fusion, the HAT selective medium (100 μL per well) was supplemented. After 5 days, the HAT medium in 96-well plates was replaced by the HT medium.…”
Section: Mouse Immunity Cell Fusion and Preparation Of Monoclonal Ant...mentioning
confidence: 99%