Abstract:At present, the prevalent Omicron mutant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has the characteristics of high infectivity and strong concealment, leading to more asymptomatic infections. The advantages...
“…The preparation of AuNPs was performed following the method of reducing chloroauric acid (0.01%) with trisodium citrate (4 mL, 1%) as described by Ye et al 29 The prepared AuNP solution was wine-red with naked eye. The solution was clear and transparent, and with no precipitation and agglomeration.…”
Norovirus is one of the main pathogens causing acute gastroenteritis worldwide, and possesses rich genetic diversity. We established a simple, fast, and suitable colloidal gold immunochromatographic detection method for common...
“…The preparation of AuNPs was performed following the method of reducing chloroauric acid (0.01%) with trisodium citrate (4 mL, 1%) as described by Ye et al 29 The prepared AuNP solution was wine-red with naked eye. The solution was clear and transparent, and with no precipitation and agglomeration.…”
Norovirus is one of the main pathogens causing acute gastroenteritis worldwide, and possesses rich genetic diversity. We established a simple, fast, and suitable colloidal gold immunochromatographic detection method for common...
“…The subtype of mAb 3G10 was ascertained using a commercial isotyping kit. The affinity of 3G10 was analyzed using a calibration curve constructed by non-competitive ELISA, and the affinity constant ( K a ) was calculated according to the following formula: 36 where n is the multiple of the concentration of any two coating antigens. Ab 2 and Ab 1 are the antibody concentration at half the maximum OD value, which correspond to two coating antigens concentration mentioned above.…”
Valproic acid (VPA) is mainly used in the clinic to treat epilepsy, for which the therapeutic window is narrow to avoid causing adverse reactions such as hepatotoxicity and teratogenicity. Therefore,...
“…SP2/0 myeloma cells and spleen cells were fused with chemical PEG, as previously conducted. [16][17][18] The growth state of the cells could be observed under a microscope and 3 days after cell fusion, the HAT selective medium (100 μL per well) was supplemented. After 5 days, the HAT medium in 96-well plates was replaced by the HT medium.…”
Section: Mouse Immunity Cell Fusion and Preparation Of Monoclonal Ant...mentioning
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