Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Coimbra, John Lennon Paiva; Dantas, Graziela de P.F.; Andrade, Lídia M.; Brener, Marcos R. G.; Viana, Pedro I.M.; Lopes, Roberta Araújo; Gontijo, Daniele O.; Ervilha, Luiz Otávio Guimarães; Assis, Mirian. Q.; Barcelos, Lucíola da Silva; Szawka, Raphael E.; Damasceno, Débora Cristina; Machado‐Neves, Mariana; Mota, Ana P.; Costa, Guilherme Mattos Jardim

doi:10.1016/j.tox.2023.153543

Cited by 7 publications

(2 citation statements)

References 131 publications

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“…Our study showed an increased level of 7-KCH as a consequence of uncontrolled lipid peroxidation in testicular cells and impaired redox balance [44]. The cytotoxic and pro-oxidant effects of nanoparticles disrupt steroid hormones metabolism [3,45].…”

Section: Discussionmentioning

confidence: 73%

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

Oczkowski,

Dziendzikowska,

Gromadzka-Ostrowska

et al. 2023

Biomedicines

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Silver nanoparticles (AgNPs) are a popular engineered nanomaterial widely used in industry. Despite the benefits they bring to society, AgNPs are not neutral to human health. The aim of this study was to evaluate the effects of a single intravenous dose (5 mg/kg body weight) of 20 nm AgNPs on steroid metabolism and redox balance in the testes of adult rats. The effects were evaluated 1 day or 28 days after intervention and compared with saline-treated animals. Decreased aromatase and estrogen receptor α levels (by 21% and 27%, respectively) were observed 1 day after AgNPs administration, while increased testosterone, increased dihydrotestosterone levels, higher androgen receptors and higher aromatase expression in Leydig cells (by 43%, 50%, 20% and 32%, respectively) as well as lower (by 35%) androgen receptor protein levels were observed 28 days after exposure to AgNPs compared to control groups. The AgNPs treatment resulted in decreased superoxide dismutase activity, decreased GSH/GSSG ratio, and increased glutathione reductase activity (by 23%, 63% and 28%, respectively) compared to control animals, irrespective of the time of measurement. Increased (by 28%) intratesticular lipid hydroperoxides level was observed 1 day after AgNPs exposure, while decreased (by 70%) GSH and increased (by 43%) 7-ketocholesterol levels were observed 28 days after treatment compared to control animals. Conclusions: AgNPs exposure caused redox imbalance in the gonads shortly after AgNPs administration, while a longer perspective AgNPs exposure was associated with impaired androgen metabolism, probably due to increased oxidative stress.

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Section: Discussionmentioning

confidence: 73%

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

Oczkowski,

Dziendzikowska,

Gromadzka-Ostrowska

et al. 2023

Biomedicines

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“…In contrast, this is not possible at higher concentrations, leading to cell cytotoxicity and the induction of inflammation [2,67]. An increase in the cytotoxicity of gold nanoparticles associated with the production of reactive oxygen species has also been observed [68][69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Effects of Spherical and Rod-like Gold Nanoparticles on the Reactivity of Human Peripheral Blood Leukocytes

Talarska,

Błaszkiewicz,

Kostrzewa

et al. 2024

Antioxidants

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Gold nanoparticles (GNPs) are widely used in the technological and biomedical industries, which is a major driver of research on these nanoparticles. The main goal of this study was to determine the influence of GNPs (at 20, 100, and 200 μg/mL concentrations) on the reactivity of human peripheral blood leukocytes. Flow cytometry was used to evaluate the respiratory burst activity and pyroptosis in monocytes and granulocytes following incubation with GNPs for 30 and 60 min. Furthermore, the concentration of interleukin-1β (IL-1β) in human blood samples was assessed using enzyme-linked immunosorbent assay (ELISA) after their incubation with GNPs for 24 h. Under the conditions tested in the study, the GNPs did not significantly affect the production of reactive oxygen species in the granulocytes and monocytes that were not stimulated using phorbol 12-myristate 13-acetate (PMA) in comparison to the samples exposed to PMA (p < 0.05). Compared to the control sample, the greatest significant increase in the mean fluorescence intensity of the granulocytes occurred in the samples incubated with CGNPs = 100 and 200 µg/mL for tinc = 30 and 60 min (p < 0.05). From our results, we conclude that the physicochemical properties of the nanoparticles, chemical composition, and the type of nanoparticles used in the unit, along with the unit and incubation time, influence the induced toxicity.

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The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

Dantas,

Ferraz,

Coimbra

et al. 2024

NanoImpact

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Gold nanoparticle intratesticular injections as a potential animal sterilization tool: Long-term reproductive and toxicological implications

Cited by 7 publications

References 131 publications

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

Does Nanosilver Exposure Modulate Steroid Metabolism in the Testes?—A Possible Role of Redox Balance Disruption

Effects of Spherical and Rod-like Gold Nanoparticles on the Reactivity of Human Peripheral Blood Leukocytes

The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

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