Gold Nanoparticles: A Versatile Label for Affinity Electrochemical Biosensors

Ambrosi, Adriano; Escosura‐Muñiz, Alfredo de la; Castañeda, María Teresa Uriarte; Merkoçi, Arben

doi:10.1002/9780470447734.ch6

Cited by 11 publications

(4 citation statements)

References 95 publications

(80 reference statements)

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“…Gold nanoparticles (AuNPs) stand out from the variety of NPs used as labels in immunosensing due to their simple synthesis, narrow size distribution, optical and electrochemical properties and easy bioconjugation alternatives. The advantageous properties of AuNP-based immuno and DNA electrochemical assays have been extensively exploited in the last few years [15,16]. The first works here were based on the NPs dissolving/destruction in aggressive acidic reagents, followed by the detection of the resulting Au (III) ions by anodic stripping voltammetry (ASV).…”

Section: Nanoparticles As Electroactive Labelsmentioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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This review shows recent trends in the use of nanoparticles as labels for electrochemical immunosensing applications. Some general considerations on the principles of both the direct detection based on redox properties and indirect detection through electrocatalytic properties, before focusing on the applications for mainly proteins detection, are given. Emerging use as blocking tags in nanochannels-based immunosensing systems is also covered in this review. Finally, aspects related to the analytical performance of the developed devices together with prospects for future improvements and applications are discussed.

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Section: Nanoparticles As Electroactive Labelsmentioning

confidence: 99%

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Iglesias-Mayor

Amor-Gutiérrez

Costa-Garcı́a

et al. 2019

Sensors

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“…Hence, alternative cost-effective methods that employ simple/user-friendly instrumentation and are able to provide adequate sensitivity and accuracy would be ideal for point-of-care diagnosis. Therefore, in recent years, there have been some attempts at cell analysis using optical-based biosensors. − In addition to optical biosensors, sensitive electrochemical DNA sensors, − immunosensors, − and other bioassays have all recently been developed by our group and others, using nanoparticles (NPs) as labels and providing direct detection without prior chemical dissolution. , …”

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confidence: 99%

Rapid Identification and Quantification of Tumor Cells Using an Electrocatalytic Method Based on Gold Nanoparticles

et al. 2009

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There is a high demand for simple, rapid, efficient, and user-friendly alternative methods for the detection of cells in general and, in particular, for the detection of cancer cells. A biosensor able to detect cells would be an all-in-one dream device for such applications. The successful integration of nanoparticles into cell detection assays could allow for the development of this novel class of cell sensors. Indeed, their application could well have a great future in diagnostics, as well as other fields. As an example of a novel biosensor, we report here an electrocatalytic device for the specific identification of tumor cells that quantifies gold nanoparticles (AuNPs) coupled with an electrotransducing platform/sensor. Proliferation and adherence of tumor cells are achieved on the electrotransducer/detector, which consists of a mass-produced screen-printed carbon electrode (SPCE). In situ identification/quantification of tumor cells is achieved with a detection limit of 4000 cells per 700 microL of suspension. This novel and selective cell-sensing device is based on the reaction of cell surface proteins with specific antibodies conjugated with AuNPs. Final detection requires only a couple of minutes, taking advantage of the catalytic properties of AuNPs on hydrogen evolution. The proposed detection method does not require the chemical agents used in most existing assays for the detection of AuNPs. It allows for the miniaturization of the system and is much cheaper than other expensive and sophisticated methods used for tumor cell detection. We envisage that this device could operate in a simple way as an immunosensor or DNA sensor. Moreover, it could be used, even by inexperienced staff, for the detection of protein molecules or DNA strands.

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“…29,30 AuNPs enable direct electron transfer between redox proteins and bulk electrode materials and are widely used in electrochemical biosensors, as well as biomolecular labels. 31,32 Magnetic nanoparticles (MNPs) MNP are often composed by iron oxide and due to their size (20 -200 nm) they can possess superparamagnetic properties. MNP are used as contrast agents for magnetic resonance imaging and for molecular separation in biosensors devices.…”

Section: Gold Nanoparticles (Aunps)mentioning

confidence: 99%

“…Among these materials, GO is the best acceptor of QD FRET donors in the solid phase. Such an ultrahigh quenching efficiency by GO and the proposed simple mechanism may open the way to several interesting applications in the field of biosensing (published in Carbon, see pages [31][32][33][34][35][36][37]. For example, it can be exploited in novel nanoenabled systems for food safety and environmental monitoring; particularly, for pathogen screening in microarray platforms.…”

Section: The Endeavour Of the Integration Of Nanomaterials Into Micro...mentioning

confidence: 99%

Nanomaterials based microarray platforms for biodetection

Narváez¹

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Analytical disciplines are an important field for the progress of healthcare and medicine. In fact the technologies related to analytical disciplines may reveal important information for early diagnosis, treatment of diseases, food safety and environmental monitoring. In this regard, novel advances in analytical disciplines are highly desired. As a promising tool, biosensors are useful systems that enable the detection of agents with diagnostic interest. Since nanotechnology enables the manipulation and control at the nanoscale, biosensors based on nanotechnology offer powerful capabilities to diagnostic technology. In this dissertation, the advantages of the integration of nanomaterials into microarray technology are widely studied, generally in terms of sensitivity. Particularly, the performance of cadmium-selenide/zinc-sulfide (CdSe@ZnS) quantum dots (QDs) and the fluorescent dye Alexa 647 as reporter in an assay designed to detect apolipoprotein E (ApoE) has been compared. The assay is a sandwich immunocomplex microarray that functions via excitation by visible light. ApoE was chosen for its potential as a biomarker for Alzheimer's disease. The two versions of the microarray (QD or Alexa 647) were assessed under the same experimental conditions. The QDs proved to be highly e¿ective reporters in the microarrays, although their performance strongly varied in function of the excitation wavelength. At 633 nm, the QD microarray, at an excitation wavelength of 532 nm, provided a limit of detection (LOD) of ~62 pg mL-1, ¿ve times more sensitive than that of the Alexa microarray (~307 pg mL-1). Finally, serial dilutions from a human serum sample were assayed with high sensitivity and acceptable precision and accuracy (Anal. Chem. 2012, 84:6821). Since graphene oxide (GO) is a recently discovered nanomaterial and microarray technology relies on optical signals, the photonic properties of GO are discussed and the state-of-the-art of GO in optical biosensing has been widely documented (Adv. Mater. 2012, 24:3298). Furthermore, GO has been studied as a highly efficient quencher of QDs, reporting a quenching efficiency of nearly 100%. Finally, such interaction between GO and QDs has been proposed as a highly sensitive transduction system for microarray-based biodetection (Carbon 2012, 50:2987). This research aims at demonstrating how the endeavour of the fusion between nanomaterials and microarray technology exhibits enormous possibilities towards biomarker screening, food safety and environmental monitoring. Las tecnologías relacionadas con el diagnóstico son un campo importante para el progreso de la medicina y el cuidado de las salud. Por ejemplo, estas tecnologías pueden aportar valiosa información para el tratamiento y diagnóstico temprano de enfermedades, seguridad en alimentos y monitoreo del medio ambiente. En este contexto, los sistemas de biosensado son una herramienta muy prometedora que permite la detección de agentes con interés diagnostico. Dado que la nanotecnología facilita la manipulación y control a la nanoescala, los sistemas de biodetección basados en nanotecnología poseen poderosas capacidades que pueden ser explotadas en las tecnologías relacionadas con el diagnóstico. En esta tesisis se han estudiado las ventajas que aporta la integración de nanomateriales a la tecnología de microarrays, generalmente en términos de sensibilidad. Particularmente, se ha estudiado el desempeño de la integración de nanocristales semiconductores (NS) para la detección de un biomarcador relacionado con Alzheimer en formato microarray. En dicho microarray se ha observado un importante rendimiento, mostrando un excelente limite de detección de 62 pg mL-1, el cual supera a otros metodos convencionales de detección como el ELISA (470 pg mL-1). También se ha analizado un banco de diluciones de una muestra de suero humano con precisión y exactitud aceptables (Anal. Chem. 2012, 84:6821). Por otra parte, ya que el óxido de grafeno (OG) es un material muy novedoso y la tecnología de microarrays depende de señales ópticas, se ha documentado ampliamente el estado del arte sobre el uso de (OG) en en el campo del biosensado óptico (Adv. Mater. 2012, 24:3298). Adicionalmente, se ha estudiado al OG como un desactivador de fluorescencia de NS altamente eficiente, presentando una eficiencia en la desactivación de NS de casi el 100%. Finalmente se ha aplicado dicha interacción entre NS y OG para diseñar un sistema de transducción altamente sensible (Carbon 2012, 50:2987 ). Esta investigación tiene por objetivo demostrar las ventajas y el potencial que posee la fusión entre los nanomateriales y la tecnología de microarrays como un sistema aplicado al campo del diagnóstico

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Gold Nanoparticles: A Versatile Label for Affinity Electrochemical Biosensors

Cited by 11 publications

References 95 publications

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Nanoparticles as Emerging Labels in Electrochemical Immunosensors

Rapid Identification and Quantification of Tumor Cells Using an Electrocatalytic Method Based on Gold Nanoparticles

Nanomaterials based microarray platforms for biodetection

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