Gold nanoparticles as an efficient drug delivery system for GLP-1 peptides

Pérez-Ortiz, M.; Zapata-Urzúa, C.; Acosta, Gerardo; Álvarez‐Lueje, A.; Alberício, Fernando; Kogan, Marcelo J.

doi:10.1016/j.colsurfb.2017.06.015

Cited by 42 publications

(20 citation statements)

References 34 publications

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“…Gold is another NP having tremendous potential for intracochlear drug delivery due to its optical and thermal properties as well as the ability to modify its size, shape and surface chemistry [73]. Gold also exhibits surface plasmon resonance making it possible to measure interactions of these NPs in real-time with high sensitivity and without the need of labels [74].…”

Section: Nps For Drug Delivery Into the Inner Earmentioning

confidence: 99%

Nanoparticle-based drug delivery in the inner ear: current challenges, limitations and opportunities

Mittal

Peña

Zhu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Hearing loss is the most common neurosensory impairment worldwide. While conductive hearing loss can be managed by surgery, the management of sensorineural hearing loss (SNHL), related to the damage of sensory cells of the inner ear is more challenging to manage medically. Many causes of SNHL such as sudden idiopathic SNHL, Meniere's disease, noise-induced hearing loss, autoimmune hearing loss or hearing loss from exposure to ototoxic substances can benefit from delivery of otoprotective drugs to the inner ear. However, systemic drug delivery through oral, intravenous and intramuscular methods leads to undesirable side effects due to the inner ear's limited blood supply and the relatively poor penetration of the blood-inner ear barrier (BLB). Therefore, there has been an increased interest for the targeted drug delivery to the inner ear using nanoparticles. Drug delivery through nanoparticles offers several advantages including drug stabilization for controlled release and surface modification for specific targeting. Understanding the biocompatibility of nanoparticles with cochlea and developing novel non-invasive delivery methods will promote the translation of nanoparticle-mediated drug delivery for auditory disorders from bench to bedside.

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Section: Nps For Drug Delivery Into the Inner Earmentioning

confidence: 99%

Nanoparticle-based drug delivery in the inner ear: current challenges, limitations and opportunities

Mittal

Peña

Zhu

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…One of the remaining challenges to the successful application of PDCs for cancer therapy is the relatively low stability of peptides to enzymatic hydrolysis in the blood, liver and kidneys [ 9 ]. One approach to overcoming this problem would be to conjugate the PDC to nanoparticles, as these have demonstrated improved pharmacokinetic and pharmacodynamics properties when used as drug delivery systems [ 10 , 11 ]. Indeed gold nanoparticles (AuNPs), with their favorable physico-chemical and safety properties [ 12 , 13 ] and ease of synthesis [ 14 ] might be appropriate agents for enhanced PDC delivery.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles stabilize peptide-drug-conjugates for sustained targeted drug delivery to cancer cells

et al. 2018

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BackgroundPeptide-drug-conjugates (PDCs) are being developed as an effective strategy to specifically deliver cytotoxic drugs to cancer cells. However one of the challenges to their successful application is the relatively low stability of peptides in the blood, liver and kidneys. Since AuNPs seem to have a longer plasma half-life than PDCs, one approach to overcoming this problem would be to conjugate the PDCs to gold nanoparticles (AuNPs), as these have demonstrated favorable physico-chemical and safety properties for drug delivery systems. We set out to test whether PEG coated-AuNPs could provide a suitable platform for the non-covalent loading of pre-formed PDCs and whether this modification would affect the bioavailability of the PDCs and their cytotoxicity toward target cancer cells.MethodsPeptides specifically internalized by A20 murine lymphoma cells were isolated from a phage library displaying 7mer linear peptides. Peptide specificity was validated by flow cytometry and confocal microscopy. PDCs were synthesized containing a selected peptide (P4) and either chlorambucil (Chlor), melphalan (Melph) or bendamustine (Bend). Gold nanoparticles were sequentially coated with citrate, PEG-6000 and then PDC (PDC-PEG-AuNP). The physico-chemical properties of the coated particles were analyzed by electrophoresis, TEM, UV–VIS and FTIR. Stability of free and PDC-coated AuNP was determined.ResultsBiopanning of the phage library resulted in discovery of several novel peptides that internalized into A20 cells. One of these (P4) was used to synthesize PDCs containing either Chlor, Melph or Bend. All three PDCs specifically killed A20 target cells, however they had short half-lives ranging from 10.6 to 15.4 min. When coated to PEG-AuNPs, the half-lives were extended to 21.0–22.3 h. The PDC-PEG-AuNPs retained cytotoxicity towards the target cells. Moreover, whereas pre-incubation for 24 h of free PDCs almost completely abolished their cytotoxic activity, the PDC-PEG-AuNPs were still active even after 72 h pre-incubation.ConclusionsPeptide-drug-conjugates hold potential for improving the target efficacy of chemotherapeutic drugs, however their short half-lives may limit their application. This hurdle can be overcome by easily conjugating them to gold nanoparticles. This conjugation also opens up the possibility of developing slow release formulations of targeted drug delivery systems containing PDCs.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0362-1) contains supplementary material, which is available to authorized users.

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“…Nanotechnology is the engineering and manufacturing of materials at the atomic and molecular scale. Recent applications of nanotechnology have been developed in peptide and bioactive compounds delivery (Madureira, Pereira, & Pintado, 2015;Pérez-Ortiz et al, 2017). Chitosan is a polycationic polymer composed of N-acetyl-D glucosamine and D-glucosamine.…”

mentioning

confidence: 99%

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

Zhao

et al. 2018

J Food Biochem

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Methodology to enhance the intestinal absorption of peptides is an important challenge due to their easily degradation and poor permeability across the intestinal epithelium. In this study, the fish‐derived peptide (DGDDGEAGKIG)‐loaded chitosan (CS) nanoparticles (CS/PEP‐NPs) were prepared and investigated in Caco‐2 monolayer model. The results indicated zeta potential of CS/PEP‐NPs increased with the increase in molecular weight of CS (10–50 kDa). Transmission electron microscopy images revealed the CS/PEP‐NPs were uniform spherical‐shaped nanoparticles with a diameter of 50–200 nm (150 kDa). Compared to other CS/PEP‐NPs, 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the intracellular fluorescence intensities of fluorescein isothiocyanate‐labeled peptide. These findings suggest that chitosan nanoparticles are promising carriers to promote intestinal absorption of fish‐derived peptide via paracellular pathway mediated by tight junctions. Practical applications Chitosans are promising carriers to promote intestinal absorption of fish‐derived peptide. The 150‐kDa CS/PEP‐NPs performed an outstanding apparent permeability coefficient (Papp, 2.29 × 10−5 cm s−1) and cumulative amount of peptide (120 min, 2,987 ng) in Caco‐2 cells. CS/PEP‐NPs could reduce the tight junction integrity of Caco‐2 cells and enhance the peptide uptake by paracellular pathway. Chitosan nanoparticles can be developed as vehicles for enhancing the cellular uptake of peptide in food industry.

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Gold nanoparticles as an efficient drug delivery system for GLP-1 peptides

Cited by 42 publications

References 34 publications

Nanoparticle-based drug delivery in the inner ear: current challenges, limitations and opportunities

Nanoparticle-based drug delivery in the inner ear: current challenges, limitations and opportunities

Gold nanoparticles stabilize peptide-drug-conjugates for sustained targeted drug delivery to cancer cells

Chitosan/sodium tripolyphosphate nanoparticles as efficient vehicles for enhancing the cellular uptake of fish-derived peptide

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