Gold nanoparticles based molecular beacons for in vitro and in vivo detection of the matriptase expression on tumor

Deng, Dawei; Zhang, Dongyin; Yang, Li; Achilefu, Samuel; Gu, Yueqing

doi:10.1016/j.bios.2013.05.018

Cited by 35 publications

(29 citation statements)

References 35 publications

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“…The peptide linker is conjugated to a fluorescent dye on one end and attached to gold nanoparticle as a fluorescent quencher on the other end so that enzymatic cleavage of the linker results in a fluorescent signal [44]. Cleavage of this peptide linker is, however, not specific to matriptase, because many other proteases possess such activity.…”

Section: Discussionmentioning

confidence: 99%

Human Cancer Cells Retain Modest Levels of Enzymatically Active Matriptase Only in Extracellular Milieu following Induction of Zymogen Activation

Chu

Yang

et al. 2014

PLoS ONE

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The type 2 transmembrane serine protease matriptase is broadly expressed in human carcinomas and hematological cancers. The proteolytic activity of matriptase is a potential target of drugs and imaging probes. We assessed the fate of active matriptase following the induction of matriptase zymogen activation. Exposing eight human carcinoma cells to pH 6.0 buffer induced robust matriptase zymogen activation followed by rapid inhibition of the nascent active matriptase by hepatocyte growth factor activator inhibitor (HAI)-1. Consequently, no enzymatically active matriptase was detected in these cells. Some active matriptase is, however, rapidly shed to the extracellular milieu by these carcinoma cells. The lack of cell-associated active matriptase and the shedding of active matriptase were also observed in two hematological cancer lines. Matriptase shedding is correlated closely with the induction of matriptase activation, suggesting that matriptase activation and shedding are kinetically coupled. The coupling allows a proportion of active matriptase to survive HAI-1 inhibition by rapid shedding from cell surface. Our study suggests that cellular free, active matriptase is scarce and might not be an effective target for in vivo imaging and drug development.

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Section: Discussionmentioning

confidence: 99%

Human Cancer Cells Retain Modest Levels of Enzymatically Active Matriptase Only in Extracellular Milieu following Induction of Zymogen Activation

Chu

Yang

et al. 2014

PLoS ONE

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“…129,130 These probes largely depend on the specificity of cleavable sequences used for the coupling of fluorophores and NPs. Numerous enzymes such as matriptase, 131 protein kinase C alpha, 132 uPA, 133 and matrix metalloproteinase (MMP), 134,135 have been successfully used to construct activatable NPs, which might also expand to novel probe design utilizing various enzymes and energy donors, as well as energy acceptors. 136 Other than enzyme activation, selective and sensitive turn-on NIRF probes can be obtained by novel strategies.…”

Section: Target-activatable Nirf Npsmentioning

confidence: 99%

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Yi¹,

Wang²,

Qin³

et al. 2014

IJN

217

149

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Near-infrared fluorescence (NIRF) imaging is an attractive modality for early cancer detection with high sensitivity and multi-detection capability. Due to convenient modification by conjugating with moieties of interests, NIRF probes are ideal candidates for cancer targeted imaging. Additionally, the combinatory application of NIRF imaging and other imaging modalities that can delineate anatomical structures extends fluorometric determination of biomedical information. Moreover, nanoparticles loaded with NIRF dyes and anticancer agents contribute to the synergistic management of cancer, which integrates the advantage of imaging and therapeutic functions to achieve the ultimate goal of simultaneous diagnosis and treatment. Appropriate probe design with targeting moieties can retain the original properties of NIRF and pharmacokinetics. In recent years, great efforts have been made to develop new NIRF probes with better photostability and strong fluorescence emission, leading to the discovery of numerous novel NIRF probes with fine photophysical properties. Some of these probes exhibit tumoricidal activities upon light radiation, which holds great promise in photothermal therapy, photodynamic therapy, and photoimmunotherapy. This review aims to provide a timely and concise update on emerging NIRF dyes and multifunctional agents. Their potential uses as agents for cancer specific imaging, lymph node mapping, and therapeutics are included. Recent advances of NIRF dyes in clinical use are also summarized.

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“…The conjugated molecules adsorbed on the surface of gold nanoparticles through their electron or hole transfer capability further modify the electrochemical properties of gold nanoparticles. [1][2][3][4][5] Gold nanoparticles coated with monolayers bearing fluorescent groups have also been found to be very useful fillers in light emitting and conducting polymers for creating hybrid organic-inorganic materials or nanocomposites with enhanced light emitting and conducting properties. [6] The preparation of gold nanoparticles coated with newly designed and synthesized fluorescent organic compounds thus becomes an important step toward preparations of hybrid gold nanoparticles for the applications mentioned above.…”

Section: Introductionmentioning

confidence: 99%

Identification of thiol from 11-(9-carbazolyl)-1-undecyl disulfide by NMR spectroscopy and single step coating of gold nanoparticles

Jadhav

Maccagno

2014

Journal of Sulfur Chemistry

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Disulfide-functionalized fluorescent derivative of carbozole named 11-(9-carbazolyl)-1-undecyl disulfide (CBZDS) has been synthesized. Proton nuclear magnetic resonance spectroscopy was used to distinguish thiol from its corresponding symmetrical disulfide. Single step synthesis of gold nanoparticles coated with CBZDS was carried out which resulted in stable monolayers of CBZDS on gold nanoparticles.

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Gold nanoparticles based molecular beacons for in vitro and in vivo detection of the matriptase expression on tumor

Cited by 35 publications

References 35 publications

Human Cancer Cells Retain Modest Levels of Enzymatically Active Matriptase Only in Extracellular Milieu following Induction of Zymogen Activation

Human Cancer Cells Retain Modest Levels of Enzymatically Active Matriptase Only in Extracellular Milieu following Induction of Zymogen Activation

Near-infrared fluorescent probes in cancer imaging and therapy: an emerging field

Identification of thiol from 11-(9-carbazolyl)-1-undecyl disulfide by NMR spectroscopy and single step coating of gold nanoparticles

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