Gold nanoparticles disturb nuclear chromatin decondensation in mouse sperm in vitro

Zakhidov, S. T.; Маршак, Т. Л.; Малолина, Е. А.; Kulibin, A. Yu.; Zelenina, I. A.; Pavluchenkova, S.M.; Рудой, В. М.; Дементьева, О. В.; Skuridin, S. G.; Evdokimov, Yu. M.

doi:10.1134/s1990747810030074

Cited by 21 publications

(28 citation statements)

References 8 publications

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“…Therefore, the results of this study support the statement, according to which the distortion of mutual orientation of neighboring quasinematic layers formed by ds DNA molecule in spatial chromosome structure, induced by fixation of Au-nanoparticles between fragments of this molecule, can be one of the reasons for genotoxicity of these particles [2,3].…”

Section: Resultssupporting

confidence: 71%

“…In conclusion, one can add, the effect of introducing Au-nanoparticles into cytoplasm of the cell has been investigated by many scientific teams (see, for instance, [1,3,7,46]). However, yet little is known about effect of Au-nanopartricles at the nucleus of the cell.…”

Section: Resultsmentioning

confidence: 99%

“…This depends mainly on the solution of two problems: 1) there are many nanomaterials with strongly different properties; 2) an adequate, "coordinated" system to test nanomaterials is not elaborated. For instance, it was suggested that biological effect of Au-nanoparticles on spatially organized double-stranded (ds) DNA structures in vitro and in vivo is similar to the effect of mutagenic molecules [2,3]. One can stress that the interaction of metal nanoparticles with native single-or double-stranded nucleic acid molecules is a well-known phenomenon [4].…”

Section: Introductionmentioning

confidence: 99%

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A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

Yevdokimov¹,

Skuridin²,

Salyanov³

et al. 2011

JBNB

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Au-nanoparticles (size about 2 nm, but not 5 or 15 nm) are capable of effectively incorporating into quasinematic layers of particles of cholesteric liquid-crystalline dispersion formed by double-stranded nucleic acid molecules of various families (DNA and poly(I)xpoly(C)). This Au-size-dependent process is accompanied by a decrease in amplitudes of abnormal bands in the CD spectra specific to initial cholesteric liquid-crystalline dispersions and simultaneously by an appearance of plasmon resonance band in visible absorption spectrum. The study of properties of particles of cholesteric liquid-crystalline dispersion treated with Au-nanoparticles by means of various physico-chemical methods demonstrates that incorporation of Au-nanoparticles into quasinematic layers of these particles results in two effects: i) it facilitates reorganization of the spatial cholesteric structure of particles, and ii) it induces the formation of Au-clusters in the content of particles. It is not excluded that these effects represent a possible reason for genotoxicity of Au-nanoparticles.

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Section: Resultssupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

Yevdokimov¹,

Skuridin²,

Salyanov³

et al. 2011

JBNB

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“…These effects were observed after incubation of spermatozoa with extremely high concentrations of ligand-free AuNPs and AgNPs (Barchanski et al, 2011;Moretti et al, 2013). This cytotoxic consequence was manifested by failure of chromatin to decondense and in the nucleus structure (Zakhidov et al, 2010). It should also be remembered that that nanoparticles can pass through the blood-testis barrier (Gao et al, 2013).…”

Section: Risk Of the Use Of Nanomaterialsmentioning

confidence: 99%

Nanobiotechnology in reproduction – pros and cons. A review

Zielińska‐Górska

Górski

2015

J. Anim. Feed Sci.

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“…Recently, the in vitro toxicity of Au NPs was even demonstrated in mouse epididymal sperm [14]. In humans, studies have been even rarer.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Effect of Gold or Silver Nanoparticles on Meiotic and Postmeiotic Fractions of Rat Germinal Cells

Collodel¹,

Terzuoli²,

Mazzi

et al. 2013

TOANDROJ

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The cytotoxicity of Au or Ag nanoparticles (NPs) on rat germinal cells was investigated in vitro. Rat germ cells separated by the STAPUT method in two different populations, pachytene spermatocytes (F5) and round spermatids (F3), were incubated (37°C, 60'-120') with 60 μM, 125 μM, 250 μM and 500 μM of Au/Ag-NPs. Cell viability was assessed with the Eosin Y test. Au or Ag-NPs were investigated with FEG-STEM/EDS and TEM. A dose-dependent effect on the viability of F3 and F5 populations was observed after incubation with Au-NPs or Ag-NPs (P<0.001). A significant decrease in cell viability was observed in F5 fractions compared to F3 fractions (P<0.05), except for the samples treated with 60μM of Au-NPs, and the decrease was also significant in all the samples treated with Ag-NPs compared to those incubated with Au-NPs (P< 0.05). Au-NPs were localized in spermatocytes and spermatids whereas Ag-NPs were undetectable. In conclusion, Au-NPs and Ag-NPs seem to exert a negative effect on rat germ cells, particularly on spermatocytes, that appeared significantly more compromised than spermatids. Further research is needed, mainly to carefully explore the possible genotoxicity of these NPs on germinal cells.

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Gold nanoparticles disturb nuclear chromatin decondensation in mouse sperm in vitro

Cited by 21 publications

References 8 publications

A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

Nanobiotechnology in reproduction – pros and cons. A review

In Vitro Effect of Gold or Silver Nanoparticles on Meiotic and Postmeiotic Fractions of Rat Germinal Cells

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