Gold nanoparticles downregulate VEGF-and IL-1β-induced cell proliferation through Src kinase in retinal pigment epithelial cells

Karthikeyan, B.; Kalishwaralal, Kalimuthu; Sheikpranbabu, Sardarpasha; Deepak, Venkataraman; Haribalaganesh, Ravinarayanan; Gurunathan, Sangiliyandi

doi:10.1016/j.exer.2010.09.003

Cited by 48 publications

(25 citation statements)

References 39 publications

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“…Administration of this DDS to breast cancer cells (MCF-7 and MDA-MB-231) showed significant inhibition of breast cancer cell proliferation compared with pristine doxorubicin. The viability of the bovine retinal pigment epithelial cells was not affected with an AuNP concentration of up to 300 nM, and increasing the concentrations above 300 nM resulted in significant cell death [62]. AuNPs have anti-oxidative and anti-hyperglycemic activities in streptozotocin-induced diabetic mice by balancing or inhibiting ROS generation in hyperglycemic conditions by scavenging free radicals and leading to increased anti-oxidant defense enzymes in mice.…”

Section: Resultsmentioning

confidence: 99%

A green chemistry approach for synthesizing biocompatible gold nanoparticles

et al. 2014

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Gold nanoparticles (AuNPs) are a fascinating class of nanomaterial that can be used for a wide range of biomedical applications, including bio-imaging, lateral flow assays, environmental detection and purification, data storage, drug delivery, biomarkers, catalysis, chemical sensors, and DNA detection. Biological synthesis of nanoparticles appears to be simple, cost-effective, non-toxic, and easy to use for controlling size, shape, and stability, which is unlike the chemically synthesized nanoparticles. The aim of this study was to synthesize homogeneous AuNPs using pharmaceutically important Ganoderma spp. We developed a simple, non-toxic, and green method for water-soluble AuNP synthesis by treating gold (III) chloride trihydrate (HAuCl4) with a hot aqueous extract of the Ganoderma spp. mycelia. The formation of biologically synthesized AuNPs (bio-AuNPs) was characterized by ultraviolet (UV)-visible absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray (EDX), dynamic light scattering (DLS), and transmission electron microscopy (TEM). Furthermore, the biocompatibility of as-prepared AuNPs was evaluated using a series of assays, such as cell viability, lactate dehydrogenase leakage, and reactive oxygen species generation (ROS) in human breast cancer cells (MDA-MB-231). The color change of the solution from yellow to reddish pink and strong surface plasmon resonance were observed at 520 nm using UV-visible spectroscopy, and that indicated the formation of AuNPs. DLS analysis revealed the size distribution of AuNPs in liquid solution, and the average size of AuNPs was 20 nm. The size and morphology of AuNPs were investigated using TEM. The biocompatibility effect of as-prepared AuNPs was investigated in MDA-MB-231 breast cancer cells by using various concentrations of AuNPs (10 to 100 μM) for 24 h. Our findings suggest that AuNPs are non-cytotoxic and biocompatible. To the best of our knowledge, this is the first report to describe the synthesis of monodispersed, biocompatible, and soluble AuNPs with an average size of 20 nm using Ganoderma spp. This study opens up new possibilities of using an inexpensive and non-toxic mushroom extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, biocompatible, and monodispersed AuNPs, which may have future diagnostic and therapeutic applications.

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Section: Resultsmentioning

confidence: 99%

A green chemistry approach for synthesizing biocompatible gold nanoparticles

et al. 2014

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“…RPE cells, together with retinal astrocytes and retinoblastoma cells are examples of cell-based assays, i.e. non-neuronal, utilized to study retinal toxicity of AuNPs [33], [72]. Prolonged microglial activation is associated with retinal degeneration and photoreceptor apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

et al. 2014

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The complex network of neuronal cells in the retina makes it a potential target of neuronal toxicity – a risk factor for visual loss. With growing use of nanoparticles (NPs) in commercial and medical applications, including ophthalmology, there is a need for reliable models for early prediction of NP toxicity in the eye and retina. Metal NPs, such as gold and silver, gain much of attention in the ophthalmology community due to their potential to cross the barriers of the eye. Here, NP uptake and signs of toxicity were investigated after exposure to 20 and 80 nm Ag- and AuNPs, using an in vitro tissue culture model of the mouse retina. The model offers long-term preservation of retinal cell types, numbers and morphology and is a controlled system for delivery of NPs, using serum-free defined culture medium. AgNO3-treatment was used as control for toxicity caused by silver ions. These end-points were studied; gross morphological organization, glial activity, microglial activity, level of apoptosis and oxidative stress, which are all well described as signs of insult to neural tissue. TEM analysis demonstrated cellular- and nuclear uptake of all NP types in all neuronal layers of the retina. Htx-eosin staining showed morphological disruption of the normal complex layered retinal structure, vacuole formation and pyknotic cells after exposure to all Ag- and AuNPs. Significantly higher numbers of apoptotic cells as well as an increased number of oxidative stressed cells demonstrated NP-related neuronal toxicity. NPs also caused increased glial staining and microglial cell activation, typical hallmarks of neural tissue insult. This study demonstrates that low concentrations of 20 and 80 nm sized Ag- and AuNPs have adverse effects on the retina, using an organotypic retina culture model. Our results motivate careful assessment of candidate NP, metallic or-non-metallic, to be used in neural systems for therapeutic approaches.

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“…The possibility of connecting the factors influencing angiogenesis is the property of carbon nanoparticles, but this has also been observed in experiments with nanosilver [40][41][42] and nanogold. [43][44][45][46] Nanosilver inhibits the phosphorylation of phosphatidylinositol-3-kinase/Akt at serine 473, leading to the inhibition of VEGF-induced angiogenesis. 41 Nanogold, by binding VEGF165 (a heparin-binding growth factor), inhibits its action and lowers the level of proliferation.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo

Grodzik¹,

Sawosz²,

Wierzbicki³

et al. 2011

IJN

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The objective of the study was to determine the effect of carbon nanoparticles produced by different methods on the growth of brain tumor and the development of blood vessels. Glioblastoma multiforme cells were cultured on the chorioallantoic membrane of chicken embryo and after 7 days of incubation, were treated with carbon nanoparticles administered in ovo to the tumor. Both types of nanoparticles significantly decreased tumor mass and volume, and vessel area. Quantitative real-time polymerase chain reaction analysis showed downregulated fibroblast growth factor-2 and vascular endothelial growth factor expression at the messenger ribonucleic acid level. The present results demonstrate antiangiogenic activity of carbon nanoparticles, making them potential factors for anticancer therapy.

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Gold nanoparticles downregulate VEGF-and IL-1β-induced cell proliferation through Src kinase in retinal pigment epithelial cells

Cited by 48 publications

References 39 publications

A green chemistry approach for synthesizing biocompatible gold nanoparticles

A green chemistry approach for synthesizing biocompatible gold nanoparticles

Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

Nanoparticles of carbon allotropes inhibit glioblastoma multiforme angiogenesis in ovo

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