Gold nanoparticles electroporation enhanced polyplex delivery to mammalian cells

Huang, Shuyan; Deshmukh, Harshavardhan; Rajagopalan, Kartik Kumar; Wang, Shengnian

doi:10.1002/elps.201300617

Cited by 26 publications

(23 citation statements)

References 50 publications

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“…These experimental studies demonstrated significant enhancement of delivery efficacy of NPs with the electroporation treatment. Huang et al (2014) and Zu et al (2014) showed that with the electroporation treatment the dominant NP delivery pathway into cells shifted from the endocytosis to the direct permeation. It should be noted that even NPs with relatively large particle size (ca.…”

Section: Physical Methods For Np Direct Deliverymentioning

confidence: 99%

See 1 more Smart Citation

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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Nanoparticles have attracted much attention as a key material for new biomedical and pharmaceutical applications. For success in these applications, the nanoparticles are required to translocate across the cell membrane and to reach to inside of the cell. Among several translocation pathways of nanoparticles, the direct permeation pathway has a great advantage due to its high delivery efficacy. However, despite many research efforts, key properties and factors for driving the direct permeation of nanoparticle and its underlying mechanisms are far from being understood. In this article, experimental and computational studies regarding the direct permeation of nanoparticles across a cell membrane will be reviewed. Firstly, experimental studies on the nanoparticle-cell interactions, where spontaneous direct permeation of nanoparticles was observed, are reviewed. From the experimental studies, potential key physico-chemical properties of nanoparticles for their direct permeation are discussed. Secondly, physical methods such as electroporation and sonoporation for delivering nanoparticles into cells are reviewed. Current status of technologies for facilitating the direct permeation of nanoparticle is presented. Finally, we review molecular dynamics simulation studies and present the latest findings on the underlying molecular mechanisms of the direct permeation of nanoparticle.

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Section: Physical Methods For Np Direct Deliverymentioning

confidence: 99%

“…The physical methods were originally developed for gene transfer into cells. Recently, these are exploited for the direct delivery of NPs (Carrasco et al, 2016;Huang et al, 2014;Kawano et al, 2006;Kim et al, 2011;Lin et al, 2009;Wang et al, 2014;Yang et al, 2011;Zu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Nakamura¹,

Watano²

2018

KONA

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“…For instance, it has been reported that the transfection efficiency of AuNPs carrying a DNA plasmid was improved approximately 1.5−2 fold when Nano Res. combined with electroporation, without inducing any toxicity [110]. In addition, Wang et al improved the delivery of gold nanorods by combining three mechanisms: Gold nanorods encapsulated in proteinshell microbubbles (AuMBs), molecular targeting (anti-VEGF receptor 2), and sonoporation employing acoustic cavitation of microbubbles (MBs) [143].…”

Section: Local Administrationmentioning

confidence: 99%

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

et al. 2015

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KEYWORDS siRNA delivery, RNA interference (RNAi)-based therapeutics, multifunctional nanoparticles, nanotoxicity of gold nanoparticles ABSTRACT RNA interference (RNAi) effectors such as small interfering RNA (siRNA) and micro RNA (miRNA) can selectively downregulate any gene implicated in the pathology of a disease. Therefore, RNAi-based therapies have immense potential for the treatment of a wide range of diseases. However, pharmacokinetic and pharmacodynamic studies have revealed that these therapeutic agents have poor bioactivity due to a number of factors, including insufficient plasma drug levels, short plasma half-lives, renal clearance, and hepatic metabolism. Non-viral delivery may facilitate the clinical application of siRNA-based therapeutics by helping to overcome these barriers. Recently, the potential of gold nanoparticles (AuNPs) as multifunctional carriers for transporting drugs, proteins, and genetic materials has been demonstrated. In this review, some of the key properties of AuNPs relevant to siRNA delivery, such as physical properties and surface chemistry have been described. In addition, the ability of AuNP-based formulation strategies to successfully overcome delivery barriers associated with siRNA, and the potential for this material to translate into safe and effective nanomedicines are critically discussed.

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“…Noble metal nanomaterials including gold and silver NPs are interesting because of their potential in biomedical applications [107]. The size and shape of metal NPs affect their physical properties and biological functions.…”

Section: Products Of Microfluidic Hf Based Synthesismentioning

confidence: 99%

Microfluidic hydrodynamic focusing for synthesis of nanomaterials

et al. 2016

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Microfluidics expands the synthetic space such as heat transfer, mass transport, and reagent consumption to conditions not easily achievable in conventional batch processes. Hydrodynamic focusing in particular enables the generation and study of complex engineered nanostructures and new materials systems. In this review, we present an overview of recent progress in the synthesis of nanostructures and microfibers using microfluidic hydrodynamic focusing techniques. Emphasis is placed on distinct designs of flow focusing methods and their associated mechanisms, as well as their applications in material synthesis, determination of reaction kinetics, and study of synthetic mechanisms.

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Gold nanoparticles electroporation enhanced polyplex delivery to mammalian cells

Cited by 26 publications

References 50 publications

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Direct Permeation of Nanoparticles across Cell Membrane: A Review

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

Microfluidic hydrodynamic focusing for synthesis of nanomaterials

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