2021
DOI: 10.1039/d0tb02309d
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Gold nanoparticles enhance fluorescence signals by flow cytometry at low antibody concentrations

Abstract: Gold nanoparticles enhance antibody-fluorescence detection by flow cytometry reducing the signal to noise ratio and improving cell biology assessments.

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Cited by 17 publications
(10 citation statements)
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“…For instance, gold nanoparticles are of particular interest due to their remarkable optical properties and neglectable associated cytotoxicity. One of their main properties is the ability to increase volume/area surface ratio, allowing immunotherapeutic antibodies binding to gold nanoparticles at very low concentrations ( 139 , 140 ).…”
Section: Immunotherapy Based On Nanotechnologymentioning
confidence: 99%
“…For instance, gold nanoparticles are of particular interest due to their remarkable optical properties and neglectable associated cytotoxicity. One of their main properties is the ability to increase volume/area surface ratio, allowing immunotherapeutic antibodies binding to gold nanoparticles at very low concentrations ( 139 , 140 ).…”
Section: Immunotherapy Based On Nanotechnologymentioning
confidence: 99%
“…The effectiveness of flow cytometry in measurement of cellular uptake of nanoparticles with SSC is well documented in previous studies. 31 For instance, Park et al compared SSC intensities of HeLa cells exposed to different types of AuNPs (40–100 nm) with the amount of intracellular AuNPs measured by inductively coupled plasma mass spectrometry (ICP-MS) and found a linear correlation between the concentration of internalized AuNPs and the SSC intensities. 32 Zucker et al confirmed the relationship between flow cytometry SSC and cellular TiO 2 NPs and AgNPs from dark field images.…”
Section: Resultsmentioning
confidence: 99%
“…This suggestion reveals that amide linkage protein molecules and sulfonated polysaccharide compound could possibly be engaged in the reduction of gold ions to NPs and the stabilization of Au-NPs in the medium. Gold nanoparticle is one of the unique materials which exhibit fluorescence behavior [41]; plasmonic property of these nanoparticulates gained significant attention owing to the signal amplification at specific frequency [42]. Here, Au-NPs showing fluorescence behavior, after conjugation with biomolecule the fluorescence, were rapidly enhanced which can be visible through the images (Figure 3 and Figures 9(a) and 9(b)).…”
Section: Xrd Analysismentioning
confidence: 92%