Gold Nanoparticles-Enhanced Amperometric Tyrosinase Biosensor Based on Three-Dimensional Sol-Gel Film-Modified Gold Electrodes

Li, Xianrui; Ren, Tiankun; Wang, Na; Ji, Xueping

doi:10.2116/analsci.29.473

Cited by 14 publications

(4 citation statements)

References 35 publications

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“…After tyrosinase enzyme was immobilized onto the Fe 3 O 4 −CH/PLL/SPCE (curve d), the peak currents obtained were smaller than that of Fe 3 O 4 −CH/PLL/SPCE (curve c). This result may be attributed to the presence of the enzyme, which hinders the access of the electrons to the electrode . Figure B shows the CVs of Ty/Fe 3 O 4 −CH/PLL/SPCE recorded at different scan rates ranging from 10 to 200 mVs −1 in 100 mM KCl solution containing 5.0 mM Fe(CN) 6 3−/4− .…”

Section: Resultsmentioning

confidence: 99%

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese

et al. 2019

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An amperometric tyramine biosensor based on poly‐L‐lysine (PLL) and Fe3O4 nanoparticles (Fe3O4NP) modified screen printed carbon electrode (SPCE) was developed. PLL was formed on the SPCE by the electropolymerization of L‐lysine. Subsequently, Fe3O4NP suspension prepared in chitosan (CH) solution was casted onto the PLL/SPCE. Tyrosinase (Ty) enzyme was immobilized onto the modified Fe3O4−CH/PLL/SPCE and the electrode was coated with Nafion to fabricate the Ty/Fe3O4−CH/PLL/SPCE. Different techniques including scanning electron microscopy, chronoamperometry (i–t curve), cyclic voltammetry and electrochemical impedance spectroscopy were utilized to study the fabrication processes, electrochemical characteristics and performance parameters of the biosensor. The analytical performance of the tyramine biosensor was evaluated with respect to linear range, sensitivity, limit of detection, repeatability and reproducibility. The response of the biosensor to tyramine was linear between 4.9×10−7–6.3×10−5 M with a detection limit of 7.5×10−8 M and sensitivity of 71.36 μA mM−1 (595 μA mM−1 cm−2). The application of the developed biosensor for the determination of tyramine was successfully tested in cheese sample and mean analytical recovery of added tyramine in cheese extract was calculated as 101.2±2.1 %. The presented tyramine biosensor is a promising approach for tyramine analysis in real samples due to its high sensitivity, rapid response and easy fabrication.

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Section: Resultsmentioning

confidence: 99%

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese

et al. 2019

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“…), that is, hydroxylation of monophenols to o ‐diphenols (monophenolase activity) and oxidation of o ‐diphenols to o ‐quinones (diphenolase activity) . Different support materials and immobilization techniques have previously been used for development of tyrosinase‐based biosensors . In the present work, a tyrosinase‐based paper biosensor is developed for determination of phenolic compounds, using a paper‐strip absorption method.…”

Section: Introductionmentioning

confidence: 99%

Development of a paper‐type tyrosinase biosensor for detection of phenolic compounds

Senyurt¹,

Eyidoğan

Yilmaz

et al. 2014

Biotech and App Biochem

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A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (L-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.

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“…Such C-S bond forming reactions not only maintain atom economy but also lead to desired products under ambient condition (23,24). Hybrid materials derived from alkoxysilane interact with proteins (25,26), modifies cell membranes (27), causes corrosion inhibition (28), used in analytical chemistry for metal detections (29,30) and are also used to make magnetic materials (31). With such diverse applications, we prepared three compounds 2-(3-(trimethoxysilyl)propylthio)naphthalene-1,4-dione (1), 2-(3-(trimethoxy silyl)propylthio)-3-methylnaphthalene-1,4-dione (2), and 2-(3-(trimethoxysilyl) propylthio)cyclohexa-2,5-diene-1,4-dione (3) as shown in the chart 1 to understand their self-assembling properties and also to find out their protein binding abilities.…”

Section: Introductionmentioning

confidence: 99%

Quinone Tethered Silylethers: Protein Binding and Film Forming Abilities

Jali

Baruah

2013

ACS Symposium Series

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The compounds 2-(3-(trimethoxysilyl)propylthio)naphthalene-1,4-dione (1), 2-(3-(trimethoxysilyl)propylthio)-3-methylnaphthalene-1,4-dione (2), and 2-(3-(trimethoxysilyl)propylthio)cyclohexa-2,5-diene-1,4-dione(3) were prepared by reacting 3-mercaptopropyl-trimethoxysilane with the corresponding quinone under ambient conditions. The protein binding abilities as well as film formation abilities of these three siloxyethers have been determined. The SEM studies have revealed that film formation by these compounds accompany formation of micron sized particles.

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Gold Nanoparticles-Enhanced Amperometric Tyrosinase Biosensor Based on Three-Dimensional Sol-Gel Film-Modified Gold Electrodes

Cited by 14 publications

References 35 publications

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese

Development of a paper‐type tyrosinase biosensor for detection of phenolic compounds

Quinone Tethered Silylethers: Protein Binding and Film Forming Abilities

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Gold Nanoparticles-Enhanced Amperometric Tyrosinase Biosensor Based on Three-Dimensional Sol-Gel Film-Modified Gold Electrodes

Cited by 14 publications

References 35 publications

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe3O4 NPs‐chitosan Composite for the Detection of Tyramine in Cheese

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe3O4 NPs‐chitosan Composite for the Detection of Tyramine in Cheese

Development of a paper‐type tyrosinase biosensor for detection of phenolic compounds

Quinone Tethered Silylethers: Protein Binding and Film Forming Abilities

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Product

Resources

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Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese

Disposable Amperometric Biosensor Based on Poly‐L‐lysine and Fe₃O₄ NPs‐chitosan Composite for the Detection of Tyramine in Cheese