Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus

Zhang, Hui; Ma, Xiaoyuan; Liu, Ying; Duan, Nuo; Wu, Shijia; Wang, Zhouping; Xu, Baocai

doi:10.1016/j.bios.2015.07.033

Cited by 252 publications

(109 citation statements)

References 24 publications

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“…Similarly, an AuNR-enhanced SERS aptasensor has also been developed for the simultaneous detection of S. typhimurium and S. aureus . 27 As shown in Fig. 7a, AuNPs were labeled with Raman reporter molecules and two types of aptamers as nanoprobes for the detection of bacterial cells.…”

Section: Aptamer-based Detection Of Bacteriamentioning

confidence: 99%

Integrating recognition elements with nanomaterials for bacteria sensing

et al. 2017

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Section: Aptamer-based Detection Of Bacteriamentioning

confidence: 99%

Integrating recognition elements with nanomaterials for bacteria sensing

et al. 2017

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“…Based on these, the LOD of 10 cells/mL can be achieved for Staphylococcus aureus detection. Similarly, through combined gold NPs (GNPs) modified with Raman molecules and Fe 3 O 4 magnetic GNPs immobilized with aptamer, Zhang et al [76] successfully fabricated GNP-enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus.…”

Section: Optical Strategiesmentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

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“…Similarly, using two different specific bonding aptamers, S. typhimurium and S. aureus were detected simultaneously at a LOD of 15 CFU/mL and 35 CFU/mL, respectively. 241 In addition, viral zoonotic pathogens, West Nile virus and Rift Valley fever virus were detected by the Au NPs coated with the SERS probes and the specific antibody. 242 It showed a LOD of 5 fg/mL in a phosphate buffered saline buffer (PBS) and 25 pg/mL in a PBS spiked fetal bovine serum.…”

Section: 212mentioning

confidence: 99%

Review—Surface-Enhanced Raman Scattering Sensors for Food Safety and Environmental Monitoring

Tang

Zhu

Meng

et al. 2018

J. Electrochem. Soc.

165

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This article gives a review of surface-enhanced Raman scattering (SERS) sensors for food safety and environmental pollution monitoring. It introduces the basic concepts of SERS substrates, SERS enhancement factors and mechanisms, SERS probes/labels, molecular recognition elements as well as optofluidic SERS devices (SERS sensors integrated with microfluidics). This article places an emphasis on the design strategies of various SERS sensors by utilizing fingerprinting SERS spectra or by using SERS probes. It highlights the applications of SERS sensors in detection of heavy metals (such as Hg 2+ , Pb 2+ , Cd 2+ and As 3+ ), inorganic anions (nitrite, nitrate, perchlorate and fluoride), toxic small molecules (polycyclic aromatic hydrocarbon, polychlorinated biphenyls, herbicides, pesticides, antibiotics and food additives), as well as pathogens (bacteria and viruses Food safety and environment pollution are among great challenges in the human society. There are some common pollutants in the environment and food, such as heavy metals, nitrites, phosphates, pesticides, herbicides, antibiotics, hormone, pathogens and other additives. Some pollutants initially are released into the environment, and then accumulate in the food chain, and enter human bodies. Uptake of pollutants by human poses a threat to human health to different extents. 1,2Hence it is essential to monitor pollutants in the environment and food. Currently pollutants are typically measured with various laboratorybased techniques, such as atomic absorption spectroscopy, atomic fluorescence spectrometry, X-ray fluorescence spectrum, inductively coupled plasma and ion-coupled plasma-mass spectroscopy, mass spectrometry, chromatography, capillary electrophoresis, analytical profile index (API), polymerase chain reaction (PCR), and enzymelinked immunosorbent assay (ELISA).3-7 Although these techniques have high sensitivity and accuracy, they rely on expensive instruments and require professional to treat samples and operate instruments in centralized laboratories. Additionally, analysis generally takes several hours to days. For example, 0.024 ppb of Hg 2+ and 0.023 ppb of As 3+ in drinking water can be detected using atomic absorption spectroscopy with a two-step electrothermal atomizer and hydride generation atomic fluorescence spectrometry, respectively. 8,9 However, they need multiple sample preconcentration processing and expensive instruments. PCR can improve the number of the target while ELISA can produce amplified recognition signal, which could both improve the detection sensitivity to pathogens. For example, oligonucleotide microarray with combination of CdSe/ZnS quantum dots as fluorescent labels shown high specificity and sensitivity of 10 colony forming units (CFU)/mL. 10 However, the complicated procedures are very time-consuming, and the method is also vulnerable to contamination. These laboratory-based techniques cannot meet the critical need of rapid on-site measurement of pollutants. Therefore, it is essential to develop portable sensors f...

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Gold nanoparticles enhanced SERS aptasensor for the simultaneous detection of Salmonella typhimurium and Staphylococcus aureus

Cited by 252 publications

References 24 publications

Integrating recognition elements with nanomaterials for bacteria sensing

Integrating recognition elements with nanomaterials for bacteria sensing

Current and Emerging Technologies for Rapid Detection of Pathogens

Review—Surface-Enhanced Raman Scattering Sensors for Food Safety and Environmental Monitoring

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