Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441

Uboldi, Chiara; Bonacchi, Daniele; Lorenzi, Giada; Hermanns, Maria Iris; Pohl, Christine; Baldi, Giovanni; Unger, Ronald E.; Kirkpatrick, C.J.

doi:10.1186/1743-8977-6-18

Cited by 168 publications

(128 citation statements)

References 37 publications

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“…However, the use of GNP synthesised through citrate method has now been found to be harmful. Presence of reducing agent sodium citrate has caused cytotoxicity and has affected the viability and proliferation of the human alveolar type-II-like cell lines A549 and NCIH441 in vitro [38]. Therefore, the GNP synthesised in this study through green process, where plant extract was used, would be safe to use for various applications.…”

Section: Direct Relation Of Gnp Size With Polyphenols and Biochemicalmentioning

confidence: 96%

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

Kumar

Yadav

2012

Journal of Experimental Nanoscience

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Here, Syzygium cumini leaf extract (LE) and seed extract (SE) were explored for the synthesis of gold nanoparticles (GNP). LE and SE as well as their polar (water) fractions showed potential for GNP synthesis. Comparative synthesis kinetics and morphological characterisation studies revealed the synthesis of smaller sized GNP by LE than SE. Only polar (water) fractions showed potential for GNP synthesis, which are smaller in size compared to their respective extracts. SE contained more polyphenols and biochemical constituents than LE and therefore, showed higher synthesis rate and bigger sized GNP. Atomic force microscope and scanning electron microscope analysis indicated that both extracts and their fractions catalysed the synthesis of spherical GNP. The average size of GNP synthesised by LE, leaf water fraction (LWF), SE and seed water fraction (SWF) were 24, 23, 35 and 32 nm, respectively. Fourier transform infrared analysis identified the biomolecules involved in the synthesis and stability of GNP. This study documented the potential of S. cumini for the synthesis of GNP in addition to silver nanoparticles (SNP). However, nature and types of polyphenols involved in GNP synthesis seem to be different from that involved in SNP synthesis. This might be the possible reason for smaller sized GNP that SNP.

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Section: Direct Relation Of Gnp Size With Polyphenols and Biochemicalmentioning

confidence: 96%

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

Kumar

Yadav

2012

Journal of Experimental Nanoscience

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“…Due to their plasmonic properties, gold nanoparticles (GNPs) have been proposed as advantageous nano-sized materials for use in the various diagnostic and therapeutic applications, such as cell imaging, targeted drug delivery, thermal ablation, phototherapy [1][2][3][4][5][6][7].. To date spherical GNPs have been proven as one of the most biocompatible NP models for biological-based applications [8][9][10][11][12]. Recently, it has been reported that the specific shape of GNPs can be exploited to advantageously tune these nanomaterials for any biomedical application of choice [8,13].…”

Section: Introductionmentioning

confidence: 99%

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

Tian

Clift

Casey³

et al. 2015

Nanomedicine (Lond.)

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Aim: To investigate the influence of gold nanoparticle (GNP) geometry on the biochemical response of Calu-3 epithelial cells. Materials and Methods:Spherical, triangular and hexagonal GNPs were used. The GNP-cell interaction was assessed via atomic absorption spectroscopy (AAS) and transmission electron microscopy (TEM). The biochemical impact of GNPs was determined over 72hrs at [0.0001-1mg/mL].Results: At 1mg/mL, hexagonal GNPs reduced Calu-3 viability below 60%, showed increased reactive oxygen species production and higher expression of pro-apoptotic markers. A cell mass burden of 1:2:12 as well as number of GNPs per cell (2:1:3) was observed for spherical:triangular:hexagonal GNPs. Conclusion:These findings do not suggest a direct shape-toxicity effect. However, do highlight the contribution of shape towards the GNP-cell interaction which impacts upon their intracellular number, mass and volume dose.

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“…Sodium citrate covered gold particles, on the contrary, werereported to get cytotoxicity. This migh be attributed to the free citrate ions within the media [33]. Similar to a surfactant type, surface properties might play a key role in the cytotoxicity of the same particles.…”

Section: On Biodecorated Gold Nanoparticles Distributed Within Tissuementioning

confidence: 99%

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

Capek¹

2015

JNMR

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Vocabulary EndocytosisIt is an energy-using process by which cells absorb molecules (such as proteins) by engulfing them. It is used by all cells of the body because most substances important to them are large polar molecules that cannot pass through the hydrophobic plasma or cell membrane. The opposite process is exocytosis. Lactate dehydrogenase (LDH)It is of medical significance because it is found extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease. The MTT assayIt is a colorimetric assay for assessing cell viability. WSTs (water soluble tetrazolium salts)These are a series of other water soluble dyes for MTT assays, developed to give different absorption spectra of the formed formazans. WST-1 is advantageous over MTT in that it is reduced outside cells. WST assays (1) can be read directly (unlike MTT that needs a solubilization step), give a more effective signal than MTT, and decrease toxicity to cells (unlike cell-permeable MTT, and its insoluble formazan that accumulate inside cells). BiotinIt also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). Biotin has an unusual structure, with two rings fused together via one of their sides. The two rings are ureido and thiophene moieties. Biotin is a heterocyclic, S-containing monocarboxylic acid.

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Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441

Cited by 168 publications

References 37 publications

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

Characterisation of gold nanoparticles synthesised by leaf and seed extract ofSyzygium cuminiL.

Investigating the Role of Shape on the Biological Impact of Gold Nanoparticles in Vitro

On Biodecorated Gold Nanoparticles Distributed within Tissues and Cells

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