Gold Nanoparticles Inhibit the Proliferation of Multiple Myeloma Cells

Bhattacharya, Resham; Patra, Chitta Ranjan; Verma, Rajanshu; Kumar, Santosh; Greipp, Philip R.; Mukherjee, Priyabrata

doi:10.1002/adma.200602098

Cited by 99 publications

(75 citation statements)

References 24 publications

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“…AuNPs inhibited the proliferation of multiple myeloma cell lines and human lung adenocarcinoma cells with IC 50 values ranging from 5-60 ppm (Bhattacharya et al, 2007;Choi et al, 2012). The amount of inhibition depends on the cell type, dose, and length of treatment.…”

Section: Discussionmentioning

confidence: 99%

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Parnsamut¹,

Brimson²

2015

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Parnsamut¹,

Brimson²

2015

Genet. Mol. Res.

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“…Gold nanoparticles (AuNPs) were synthesized from tetrachlorauric acid by wet chemical methods using sodium borohydride as a reducing agent as previously described in ref. 36. Briefly, an aqueous stock solution of tetrachloroauric acid (HAuCl 4 ) was reduced with an aqueous solution of sodium borohydride (NaBH 4 ) under vigorous stirring (18).…”

Section: Discussionmentioning

confidence: 99%

Nanoconjugation modulates the trafficking and mechanism of antibody induced receptor endocytosis

Bhattacharyya¹,

Bhattacharya²,

Curley

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

124

123

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Treatment with monoclonal antibody (mAbs) is a viable therapeutic option in cancer. Recently, these mAbs such as cetuximab, herceptin, etc., have been used as targeting agents to selectively deliver chemotherapeutics to cancerous cells. However, mechanisms of nanoparticles-mAbs interactions with the target cells and its effect on intracellular trafficking and mechanism are currently unknown. In this paper, we demonstrate that the distinct patterning and dynamics of anti-EGFR (epidermal growth factor receptor) antibody cetuximab (C225)-induced EGFR internalization in pancreatic cancer cells with variable receptor expression is altered upon nanoconjugation. Nanoconjugation uniformly enhanced C225-induced EGFR endocytosis in PANC-1, AsPC-1, and MiaPaca-2 cells, influenced its compartmentalization and regulated the involvement of dynamin-2 in the endocytic processes. Receptor endocytosis and its intracellular trafficking were monitored by confocal microscopy and transmission electron microscopy. The role of dynamin-2 in EGFR endocytosis was determined after overexpressing either wild-type dynamin-2 or mutant dynamin-2 in pancreatic cancer cells followed by tracking the receptor-antibody complex internalization by confocal microscopy. Significantly, these findings demonstrate that the nanoconjugation cannot be construed as an innocuous reaction involved in attaching the targeting agent to the nanoparticle, instead it may distinctly alter the cellular processes at the molecular level, at least antibody induced receptor endocytosis. This information is critical for successful design of a nanoparticle-based targeted drug delivery system for future clinical translation.EGFR | nanoparticle | dynamin | pancreatic cancer N anotechnology provides opportunities for biomedical applications that may improve human health care in the future (1-13). Engineered nanoparticles are evolving as promising candidates for various biological applications due to their tunable biophysical and biochemical properties (14-16). However, fundamental studies to understand the mechanism of cell-nanomaterial interactions are still lacking. To advance the successful development of biomedical nanotechnology for clinical use, vivid understanding of such mechanisms is essential.Epidermal growth factor receptor (EGFR) represents a unique target as over expression of EGFR has been implicated in the pathogenesis of many cancers (17, 18). Cetuximab (C225), a monoclonal anti-EGFR antibody, has been approved by the FDA for treatment of colon and head and neck cancers (19). Cetuximab is also in different phases of clinical trials for other cancers (20,21). However, the mechanism and pattern of C225 induced endocytosis of EGFR in pancreatic cancer cells of variable EGFR expression is not fully understood (20,21). Furthermore, the effect of nanoconjugation on the mechanism of C225 induced endocytosis remains to be investigated. Here, we demonstrate the mechanism and endocytic pattern of C225 and gold conjugated-C225 induced internalization of EGFR in pancreatic ...

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“…40 Another group reported that GNPs can inhibit heparin-binding growth-factor (VEGF/bFGF)-mediated cell proliferation and disturbance of cell cycle because the binding of GNPs to their growth factors can efficiently block their functions to promote proliferation. [41][42] Latest work by Deng et al demonstrated that poly(acrylic acid)-coated GNPs (PAA-GNPs) can induce strong inflammatory response after binding to and inducing unfolding of fibrinogen in plasma, while PAA-GNPs and fibrinogen themselves do not trigger this response. 43 Detailed investigation pointed that 5 nm PAA-GNPs can insert into chains of fibrinogen by electrostatic interaction.…”

Section: Influences On Physiological Functionsmentioning

confidence: 99%

Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

Jiang

Wang

Chen

2011

J Chinese Chemical Soc

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Promising application of gold nanoparticles (GNPs) in biomedical and environmental fields is increasing the likelihood of direct interaction with living systems. The knowledge about GNPs interacting with cells is quite limited and however, is necessary for risk prediction and evaluation of biological effects. Herein, we review recent advances in cellular effects of GNPs including cellular uptake, trafficking, subcellular distribution and cellular responses, and the relationship between physiochemical properties of GNPs and biological effects as well. To uncover these issues, it is quite instructive for safe and sustainable use of GNPs.

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Gold Nanoparticles Inhibit the Proliferation of Multiple Myeloma Cells

Cited by 99 publications

References 24 publications

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Effects of silver nanoparticles and gold nanoparticles on IL-2, IL-6, and TNF-α production via MAPK pathway in leukemic cell lines

Nanoconjugation modulates the trafficking and mechanism of antibody induced receptor endocytosis

Cellular Uptake, Intracellular Trafficking and Biological Responses of Gold Nanoparticles

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