Gold-nanorod contrast-enhanced photoacoustic micro-imaging of focused-ultrasound induced blood-brain-barrier opening in a rat model

Wang, Po‐Hsun; Liu, Hao-Li; Hsu, Po-Hung; Lin, Chi‐Chang; Wang, Churng-Ren Chris; Chen, Pin-Yuan; Wei, Kuo-Chen; Yen, Tzu‐Chen; Li, Menglin

doi:10.1117/1.jbo.17.6.061222

Cited by 53 publications

(33 citation statements)

References 36 publications

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“…In addition to active transport, the BBB can also be chemically or physically opened to allow the free diffusion of contrast agents [90]. Li et al used IRDye800-c(KRGDf) to target the overexpressed integrin α v β 3 in brain glioblastoma [61].…”

Section: Photoacoustic Molecular Imaging In the Brainmentioning

confidence: 99%

Photoacoustic brain imaging: from microscopic to macroscopic scales

Yao¹,

Wang²

2014

Neurophoton

171

127

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Human brain mapping has become one of the most exciting contemporary research areas, with major breakthroughs expected in the following decades. Modern brain imaging techniques have allowed neuroscientists to gather a wealth of anatomic and functional information about the brain. Among these techniques, by virtue of its rich optical absorption contrast, high spatial and temporal resolutions, and deep penetration, photoacoustic tomography (PAT) has attracted more and more attention, and is playing an increasingly important role in brain studies. In particular, PAT complements other brain imaging modalities by providing high-resolution functional and metabolic imaging. More importantly, PAT’s unique scalability enables scrutinizing the brain at both microscopic and macroscopic scales, using the same imaging contrast. In this Review, we present the state-of-the-art PAT techniques for brain imaging, summarize representative neuroscience applications, outline the technical challenges in translating PAT to human brain imaging, and envision potential technological deliverables.

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Section: Photoacoustic Molecular Imaging In the Brainmentioning

confidence: 99%

Photoacoustic brain imaging: from microscopic to macroscopic scales

Yao¹,

Wang²

2014

Neurophoton

171

127

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“…In the case of NRs, the two characteristic SPR modes are transverse and longitudinal SPR or normal and parallel to the long axes of the NRs, respectively, and take place at specific wavelength bands, called plasmon bands [1][2] . For noble metals, the plasmon bands are positioned in the UV -Visible range, features that are advantageous in numerous applications [3][4][5][6][7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Enhanced anisotropy of gold nanorods-polymer composite films for optical applications

Stoenescu¹,

Bădilescu²,

Packirisamy³

et al. 2012

Photonics North 2012

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The strong optical absorption, scattering and local electric field enhancement associated with the longitudinal Surface Plasmon Resonance (SPR) of gold nanorods (AuNRs) have important applications in imaging, sensing, nonlinear optics, thermal therapy and data encoding. The longitudinal SPR mode can be optimally excited only in the NRs that are most aligned with the electric field of a linearly polarized incident light. Thus, in cast polymer based nanorod composite films, where the NRs orientation is random, only a fraction of the embedded NRs is actually usable to the maximal extent for the intended applications. To enhance the degree of alignment of the AuNRs by uniaxial stretching and increase the application efficiency, we have improved the polymer matrix with respect to plastic deformation and designed a suitable drawing device to reduce the fracture risks of the polymer. The resulting nanocomposite film was characterized by Scanning Electron Microscopy (SEM) and by spectroscopy using linearly polarized light in the UV-Visible range. The linear dichroic ratio of the stretched nanocomposite film was calculated based on the ratio of the peak absorbance of the incident light parallelly polarized, to that of the light polarized perpendicularly to the NRs long axes.

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“…Other methods besides MRI have been used to detect FUS-induced BBB opening, such as single photon emission computed tomography (SPECT) with i.v. administration of 99m TC-DTPA radiotracer as the contrast agent, or small-animal photoacoustic imaging with gold nanorods 15, 16. In addition, dynamic changes in microvasculature during FUS-induced BBB opening have been microscopically observed by fluorescent multi-photon microscopy, and with a high-speed camera 17, 18.…”

Section: Introductionmentioning

confidence: 99%

Contrast-Enhanced Ultrasound Imaging for the Detection of Focused Ultrasound-Induced Blood-Brain Barrier Opening

et al. 2014

Self Cite

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The blood-brain barrier (BBB) can be transiently and locally opened by focused ultrasound (FUS) in the presence of microbubbles (MBs). Various imaging modalities and contrast agents have been used to monitor this process. Unfortunately, direct ultrasound imaging of BBB opening with MBs as contrast agent is not feasible, due to the inability of MBs to penetrate brain parenchyma. However, FUS-induced BBB opening is accompanied by changes in blood flow and perfusion, suggesting the possibility of perfusion-based ultrasound imaging. Here we evaluated the use of MB destruction-replenishment, which was originally developed for analysis of ultrasound perfusion kinetics, for verifying and quantifying FUS-induced BBB opening. MBs were intravenously injected and the BBB was disrupted by 2 MHz FUS with burst-tone exposure at 0.5-0.7 MPa. A perfusion kinetic map was estimated by MB destruction-replenishment time-intensity curve analysis. Our results showed that the scale and distribution of FUS-induced BBB opening could be determined at high resolution by ultrasound perfusion kinetic analysis. The accuracy and sensitivity of this approach was validated by dynamic contrast-enhanced MRI. Our successful demonstration of ultrasound imaging to monitor FUS-induced BBB opening provides a new approach to assess FUS-dependent brain drug delivery, with the benefit of high temporal resolution and convenient integration with the FUS device.

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Gold-nanorod contrast-enhanced photoacoustic micro-imaging of focused-ultrasound induced blood-brain-barrier opening in a rat model

Cited by 53 publications

References 36 publications

Photoacoustic brain imaging: from microscopic to macroscopic scales

Photoacoustic brain imaging: from microscopic to macroscopic scales

Enhanced anisotropy of gold nanorods-polymer composite films for optical applications

Contrast-Enhanced Ultrasound Imaging for the Detection of Focused Ultrasound-Induced Blood-Brain Barrier Opening

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